Insights into evolutionary trends in molecular biology tools in microbial screening for biohydrogen production through dark fermentation

Kumar, Gopalakrishnan; Cho, Si-Kyung; Sivagurunathan, Periyasamy; Anburajan, Parthiban; Mahapatra, Durga Madhab; Park, Jeong Hoon; Pugazhendhi, Arivalagan

doi:10.1016/j.ijhydene.2018.09.040

Cited by 45 publications

(13 citation statements)

References 145 publications

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“…An increased biodiversity was reported also by Józefiak et al (2019a), through the fluorescent in situ hybridisation, including 20% of full-fat BSF prepupae in rainbow trout diet. This hybridisation technique based on nucleic acids allows the identification of microbial species using group or species-specific fluorescent labelled oligoprobes avoiding the use of DNA extraction and polymerase chain reaction (PCR) (Kumar et al, 2018).…”

Section: Microbiomementioning

confidence: 99%

Application of laboratory methods for understanding fish responses to black soldier fly (Hermetia illucens) based diets

Zarantoniello

Randazzo

Secci

et al. 2022

JIFF

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A major challenge for development of sustainable aquafeeds is its dependence on fish meal and fish oil. Replacement with more sustainable, nutritious and safe ingredients is now a priority. Over the last years, among several alternatives proposed, insects have received great attention as possible candidates. In particular, the black soldier fly (Hermetia illucens; BSF) represents a concrete example of how the circular economy concept can be applied to fish culture, providing a valuable biomass rich in fat and protein valorising organic by-products. In the last decade, several studies have been published about the use of different BSF dietary inclusion levels for various fish species including experimental models. Varying and encouraging results have been obtained in this research field using a plethora of laboratory methodological approaches that can be applied and coupled to obtain a comprehensive view of the BSF-based diets effects on fish physiology, health, and quality. The present review aims to explore some of the most promising laboratory approaches like histology, infrared spectroscopy, gut microbiome sequencing, molecular biology, fish fillets’ physico-chemical and sensory properties, essential for a better understanding of fish welfare and fillet quality, when BSF is used as aquafeed ingredient. In particular, great importance has been given to European finfish species and experimental models.

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Section: Microbiomementioning

confidence: 99%

Application of laboratory methods for understanding fish responses to black soldier fly (Hermetia illucens) based diets

Zarantoniello

Randazzo

Secci

et al. 2022

JIFF

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“…and Clostridium sp. In the case of hydrogen (H 2 ) production, acetogens are the superior community in the media, but H 2 -producing bacteria control the biofilm production [60]. For the production of acetate, Acetobacterium sp.…”

Section: Electrosynthesis Assisted By Microbesmentioning

confidence: 99%

Valorisation of CO2 into Value-Added Products via Microbial Electrosynthesis (MES) and Electro-Fermentation Technology

et al. 2021

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Microbial electrocatalysis reckons on microbes as catalysts for reactions occurring at electrodes. Microbial fuel cells and microbial electrolysis cells are well-known in this context; both prefer the oxidation of organic and inorganic matter for producing electricity. Notably, the synthesis of high energy-density chemicals (fuels) or their precursors by microorganisms using bio-cathode to yield electrical energy is called Microbial Electrosynthesis (MES), giving an exceptionally appealing novel way for producing beneficial products from electricity and wastewater. This review accentuates the concept, importance and opportunities of MES, as an emerging discipline at the nexus of microbiology and electrochemistry. Production of organic compounds from MES is considered as an effective technique for the generation of various beneficial reduced end-products (like acetate and butyrate) as well as in reducing the load of CO2 from the atmosphere to mitigate the harmful effect of greenhouse gases in global warming. Although MES is still an emerging technology, this method is not thoroughly known. The authors have focused on MES, as it is the next transformative, viable alternative technology to decrease the repercussions of surplus carbon dioxide in the environment along with conserving energy.

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“…Many of these studies on microbial communities were conducted with polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE). While PCR-DGGE can provide qualitative information on the microbial community, it has the limitation of separating relatively short DNA fragments (~500 bp) [13] and minor constituents of the community may not be identified [14]. High-throughput sequencing offers the solution to this problem, as the method provides quantitative analysis and higher coverage, so the minor constituents of the community can be identified.…”

Section: Introductionmentioning

confidence: 99%

Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond

Taroepratjeka

Imai

Chairattanamanokorn

et al. 2021

Fuels

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Extreme halophiles offer the advantage to save on the costs of sterilization and water for biohydrogen production from lignocellulosic waste after the pretreatment process with their ability to withstand extreme salt concentrations. This study identifies the dominant hydrogen-producing genera and species among the acclimatized, extremely halotolerant microbial communities taken from two salt-damaged soil locations in Khon Kaen and one location from the salt evaporation pond in Samut Sakhon, Thailand. The microbial communities’ V3–V4 regions of 16srRNA were analyzed using high-throughput amplicon sequencing. A total of 345 operational taxonomic units were obtained and the high-throughput sequencing confirmed that Firmicutes was the dominant phyla of the three communities. Halanaerobium fermentans and Halanaerobacter lacunarum were the dominant hydrogen-producing species of the communities. Spatial proximity was not found to be a determining factor for similarities between these extremely halophilic microbial communities. Through the study of the microbial communities, strategies can be developed to increase biohydrogen molar yield.

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Insights into evolutionary trends in molecular biology tools in microbial screening for biohydrogen production through dark fermentation

Cited by 45 publications

References 145 publications

Application of laboratory methods for understanding fish responses to black soldier fly (Hermetia illucens) based diets

Application of laboratory methods for understanding fish responses to black soldier fly (Hermetia illucens) based diets

Valorisation of CO2 into Value-Added Products via Microbial Electrosynthesis (MES) and Electro-Fermentation Technology

Extremely Halophilic Biohydrogen Producing Microbial Communities from High-Salinity Soil and Salt Evaporation Pond

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