Insights into colloidal nanoparticle-protein corona interactions for nanomedicine applications

Martínez-Negro, María; González‐Rubio, Guillermo; Aicart, Emilio; Landfester, Katharina; Guerrero‐Martínez, Andrés; Junquera, Elena

doi:10.1016/j.cis.2021.102366

Cited by 41 publications

(32 citation statements)

References 166 publications

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“…Given the differences in the evolution of protein profiles as a function of protein concentration, we decided to investigate the composition of the protein corona to identify stealth and biosafe protein coatings for in vivo applications. 21 According to the literature, 19 we characterized protein corona at low (HP = 5%) and high (HP = 50%) protein concentrations, where the largest difference in physical-chemical properties of nanoparticle–protein complexes is typically found. However, as Figure 3 a shows, the size of PL-protein complexes at low HP was too large ( D H > 500 nm) to be compatible with drug delivery purposes.…”

Section: Resultsmentioning

confidence: 99%

Opsonin-Deficient Nucleoproteic Corona Endows UnPEGylated Liposomes with Stealth Properties In Vivo

et al. 2022

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For several decades, surface grafted polyethylene glycol (PEG) has been a go-to strategy for preserving the synthetic identity of liposomes in physiological milieu and preventing clearance by immune cells. However, the limited clinical translation of PEGylated liposomes is mainly due to the protein corona formation and the subsequent modification of liposomes’ synthetic identity, which affects their interactions with immune cells and blood residency. Here we exploit the electric charge of DNA to generate unPEGylated liposome/DNA complexes that, upon exposure to human plasma, gets covered with an opsonin-deficient protein corona. The final product of the synthetic process is a biomimetic nanoparticle type covered by a proteonucleotidic corona, or “proteoDNAsome”, which maintains its synthetic identity in vivo and is able to slip past the immune system more efficiently than PEGylated liposomes. Accumulation of proteoDNAsomes in the spleen and the liver was lower than that of PEGylated systems. Our work highlights the importance of generating stable biomolecular coronas in the development of stealth unPEGylated particles, thus providing a connection between the biological behavior of particles in vivo and their synthetic identity.

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Section: Resultsmentioning

confidence: 99%

Opsonin-Deficient Nucleoproteic Corona Endows UnPEGylated Liposomes with Stealth Properties In Vivo

et al. 2022

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“…[ 99 ] Nevertheless, the formation of a protein corona may be beneficial and can be used to alter the physiological behavior of drug NCs. [ 97 ] Thus, a variety of experimental methods including FCS have been employed to get insights into the protein corona formation. [ 100 ] In particular, FCS was applied to investigate protein–nanoparticle interactions under both model (e.g., protein solutions) and physiological (e.g., blood serum) conditions.…”

Section: Nanocarriers In Biological Fluidsmentioning

confidence: 99%

Shining Light on Polymeric Drug Nanocarriers with Fluorescence Correlation Spectroscopy

Schmitt

Nuhn

Barz

et al. 2022

Macromol. Rapid Commun.

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The use of nanoparticles as carriers is an extremely promising way for administration of therapeutic agents, such as drug molecules, proteins, and nucleic acids. Such nanocarriers (NCs) can increase the solubility of hydrophobic compounds, protect their cargo from the environment, and if properly functionalized, deliver it to specific target cells and tissues. Polymer‐based NCs are especially promising, because they offer high degree of versatility and tunability. However, in order to get a full advantage of this therapeutic approach and develop efficient delivery systems, a careful characterization of the NCs is needed. This review highlights the fluorescence correlation spectroscopy (FCS) technique as a powerful and versatile tool for NCs characterization at all stages of the drug delivery process. In particular, FCS can monitor and quantify the size of the NCs and the drug loading efficiency after preparation, the NCs stability and possible interactions with, e.g., plasma proteins in the blood stream and the kinetic of drug release in the cytoplasm of the target cells.

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“…The field of nanomedicine has largely motivated the study of nanoparticle–protein interactions to improve nanoparticle function in applications including drug delivery, disease diagnostics, treatment, and prevention. − Nanoparticle-based sensors have been used to detect metal ions, small molecules, and proteins including biomarkers for early cancer and kidney disease. − As nanotechnologies become more widely used in biological settings, it is increasingly important to understand and predict nanoparticle function and fate in vivo ; despite the successes of some, many nanomaterials produce unsatisfactory results or off-target effects during clinical trials. − This translation of nanomedicines from laboratory development to clinical practice is limited by our lack of control over interactions between the nanoparticle and its surrounding bioenvironment. ,− Although the intrinsic physicochemical characteristics of the nanoparticle determine in vitro functionality, the environment around the nanoparticle in the applied setting, such as blood plasma for intravenous delivery, will play a dominant role in determining the ultimate nanoparticle fate and function.…”

Section: Nanoparticle-based Sensors For Agricultural Innovations and ...mentioning

confidence: 99%

In Planta Nanosensors: Understanding Biocorona Formation for Functional Design

et al. 2021

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Climate change and population growth are straining agricultural output. To counter these changes and meet the growing demand for food and energy, the monitoring and engineering of crops are becoming increasingly necessary. Nanoparticle-based sensors have emerged in recent years as new tools to advance agricultural practices. As these nanoparticle-based sensors enter and travel through the complex biofluids within plants, biomolecules including proteins, metabolites, lipids, and carbohydrates adsorb onto the nanoparticle surfaces, forming a coating known as the "bio-corona". Understanding these nanoparticle−biomolecule interactions that govern nanosensor function in plants will be essential to successfully develop and translate nanoparticle-based sensors into broader agricultural practice.

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Insights into colloidal nanoparticle-protein corona interactions for nanomedicine applications

Cited by 41 publications

References 166 publications

Opsonin-Deficient Nucleoproteic Corona Endows UnPEGylated Liposomes with Stealth Properties In Vivo

Opsonin-Deficient Nucleoproteic Corona Endows UnPEGylated Liposomes with Stealth Properties In Vivo

Shining Light on Polymeric Drug Nanocarriers with Fluorescence Correlation Spectroscopy

In Planta Nanosensors: Understanding Biocorona Formation for Functional Design

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