Insights into circular RNAs: their biogenesis, detection, and emerging role in cardiovascular disease

Ward, Zoe; Pearson, John; Schmeier, Sebastian; Cameron, Vicky A; Pilbrow, Anna P.

doi:10.1080/15476286.2021.1891393

Cited by 17 publications

(20 citation statements)

References 150 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ideal biomarker should possess the following characteristics: high specificity, high sensitivity, non-invasive, convenient, cheap, and reproducible. Because of their unique structure, high stability, and specific expression, circRNAs are being increasingly recognized as new diagnostic markers of diseases in recent years ( Zhou et al, 2020 ; Wang J. et al, 2021 ; Ward et al, 2021 ). For example, circ-PSD3 in tissue samples may be a potential diagnostic biomarker or molecular therapy target for papillary thyroid carcinoma ( Li Z. et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…Circular RNAs (circRNAs) are a new type of non-coding RNA molecule, which are composed of exons, introns, or fragments of the two, and are highly resistant to degradation because of their distinct circular structure ( Zhou et al, 2020 ). Emerging evidence suggests that circRNAs are potential candidates for diagnostic biomarkers and therapeutic targets for a variety of diseases, including tumors ( Wang J. et al, 2021 ), cardiovascular diseases ( Ward et al, 2021 ), and nervous system diseases ( Li M.-L. et al, 2021 ). However, at present, knowledge of circRNAs in gout remains limited.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout

et al. 2021

View full text Add to dashboard Cite

Circular RNAs (circRNAs) are non-coding RNAs (ncRNAs) with a single-stranded covalently closed-loop structure, and their abnormal expression may participate in the pathogenesis of various human diseases. Currently, knowledge of circRNAs in gout is limited. In this case-control study, human circRNA microarrays were used to identify differentially expressed circRNAs in peripheral blood mononuclear cells (PBMCs) from patients with primary gout (n = 5) and healthy controls (HC; n = 3). Bioinformatics methods were used to analyze significantly different circRNAs (fold change >1.5, p < 0.05). In addition, four significantly differentially expressed circRNAs were selected for quantitative real-time polymerase chain reaction to detect expression levels in 90 gout patients and 60 HC. Subsequently, circRNA-miRNA-mRNA network was established to predict the function of circRNAs of interest. Microarray analysis indicated that 238 circRNAs were upregulated and 41 circRNAs were down-regulated in the gout group (fold change >1.5, p < 0.05). Bioinformatics analysis showed that differentially expressed circRNAs were involved in the pathogenesis of gout via various pathways. Moreover, the expression levels of hsa_circRNA_103657 and hsa_circRNA_000241 were significantly higher in the gout group than those in the HC group, and both correlated significantly with lipid metabolism parameters. Furthermore, the area under the curve of hsa_circRNA_103657 was 0.801 (95% confidence interval (CI): 0.730–0.871; p < 0.001). Our results provide novel insights into the pathogenesis of primary gout. Differentially expressed circRNAs were identified in the PBMCs of gout patients, and these differential circRNAs may play important roles in the development and progression of gout.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In addition, studies have suggested that miRNAs, as molecular 'switches' that regulate genes (Fichtlscherer et al, 2010;Chen and Zhou, 2011), play an important role in regulating angiogenesis (Ward et al, 2021). As an endothelium-specific miRNA, miR-126 plays an important regulatory role in angiogenesis.…”

Section: Platelet Lipidomics Related To Atherosclerosismentioning

confidence: 99%

Mechanism and Potential Target of Blood-Activating Chinese Botanical Drugs Combined With Anti-Platelet Drugs: Prevention and Treatment of Atherosclerotic Cardiovascular Diseases

Yang

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

Atherosclerotic cardiovascular diseases (ASCVDs) are the most important diseases that endanger people’s health, leading to high morbidity and mortality worldwide. In addition, various thrombotic events secondary to cardiovascular and cerebrovascular diseases need must be considered seriously. Therefore, the development of novel anti-platelet drugs with high efficiency, and fewer adverse effects has become a research focus for preventing of cardiovascular diseases (CVDs). Blood-activation and stasis-removal from circulation have been widely considered as principles for treating syndromes related to CVDs. Blood-activating Chinese (BAC botanical drugs, as members of traditional Chinese medicine (TCM), have shown to improve hemodynamics and hemorheology, and inhibit thrombosis and atherosclerosis. Modern medical research has identified that a combination of BAC botanical drugs and anti-platelet drugs, such as aspirin or clopidogrel, not only enhances the anti-platelet effects, but also reduces the risk of bleeding and protects the vascular endothelium. The anti-platelet mechanism of Blood-activating Chinese (BAC) botanical drugs and their compounds is not clear; therefore, their potential targets need to be explored. With the continuous development of bioinformatics and “omics” technology, some unconventional applications of BAC botanical drugs have been discovered. In this review, we will focus on the related targets and signaling pathways of anti-atherosclerotic treatments involving a combination of BAC botanical drugs and anti-platelet drugs reported in recent years.

show abstract

“…CircRNAs lack polyadenylated (poly(A)) tails and possess a nonlinear conformation, which is abolished after the poly(A) + enrichment step in RNA-seq profiling. Currently, the widely used Ribo-Zero approach (which facilitates ribosomal RNA (rRNA) depletion) in sequencing library construction led to the discovery of thousands of circRNAs [ 40 ]. Furthermore, circRNAs can be enriched by the additional application of the 3′-5′ exonuclease Ribonuclease R (RNase R), which degrades linear RNA [ 41 ].…”

Section: Research Approaches For Circrnasmentioning

confidence: 99%

Biological functions, mechanisms, and clinical significance of circular RNA in pancreatic cancer: a promising rising star

Chen

Shen

et al. 2022

Cell Biosci

View full text Add to dashboard Cite

Pancreatic cancer (PC) is a highly malignant solid tumor with insidious onset and easy early metastasis. Despite tremendous efforts devoted to research in this field, the mechanisms underlying PC tumorigenesis and progression remain unclear. Additionally, robust biomarkers and satisfactory therapeutic strategies for clinical use in PC patients are still lacking. Circular RNAs (circRNAs) are a new type of non-coding RNA originating from precursor messenger RNAs, with a covalent continuous closed-loop structure, strong stability and high specificity. Accumulating evidence suggests that circRNAs may participate in PC development and progression. Abnormal expression of circRNAs in PC is considered a vital factor that affects tumor cell proliferation, migration, invasion, apoptosis, angiogenesis and drug resistance. In this review of relevant articles published in recent years, we describe the basic knowledge concerning circRNAs, including their classification, biogenesis, functions and research approaches. Moreover, the biological roles and clinical significance of circRNAs related to PC are discussed. Finally, we note the questions remaining from recent studies and anticipate that further investigations will address these gaps in knowledge in this field. In conclusion, we expect to provide insights into circRNAs as potential targets for specific PC diagnosis and treatment in the future.

show abstract

Insights into circular RNAs: their biogenesis, detection, and emerging role in cardiovascular disease

Cited by 17 publications

References 150 publications

Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout

Expression Profile and Potential Function of Circular RNAs in Peripheral Blood Mononuclear Cells in Male Patients With Primary Gout

Mechanism and Potential Target of Blood-Activating Chinese Botanical Drugs Combined With Anti-Platelet Drugs: Prevention and Treatment of Atherosclerotic Cardiovascular Diseases

Biological functions, mechanisms, and clinical significance of circular RNA in pancreatic cancer: a promising rising star

Contact Info

Product

Resources

About