Insights into circular RNA biology

Ebbesen, Karoline K; Hansen, Thomas B.; Kjems, Jørgen

doi:10.1080/15476286.2016.1271524

Cited by 383 publications

(288 citation statements)

References 74 publications

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“…As reported, circRNAs could function as a miRNA sponge to regulate the gene expression [26, 27] and be involved in cancer processes [28-30]. To dissect the selected circRNAs’ potential functions, the circRNA and miRNA interactions were predicted by using Arraystar’s homemade miRNA target prediction software based on TargetScan and miRanda.…”

Section: Resultsmentioning

confidence: 99%

CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients

Zhao

Wang

et al. 2017

Cell Physiol Biochem

105

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Background/Aims: Lung adenocarcinoma, a form of non-small cell lung cancer with high lethality at an advanced stage, is becoming more common in women, non- or never-smokers, and even young adults. At present, there are still no effective early diagnosis methods for patients to be cured in a timely manner. Circular RNAs (circRNAs), which are stable and conserved non-coding RNA in mammalian cells, have been reported to be widely involved in the processes of cancer disease. However, it is still a puzzle as to which specific circRNAs are involved in the development of early-stage lung adenocarcinoma. Methods: Tumor samples and paired adjacent normal tissues from 4 patients with early-stage lung adenocarcinoma were selected to investigate the expression profile of circRNAs by using a high-throughput circRNA microarray. Bioinformatic analyses were conducted to screen those differentially expressed circRNAs. qRT-PCR and sequencing were performed to assure the microarray data. Results: A total of 357 circRNAs were dysregulated in the tumor samples, which suggests potential roles in lung cancer. qRT-PCR detection showed that five selected circRNAs were identical to the microarray data, and the potential circRNA-miRNA interactions were predicted. Conclusion: This work illustrates that clusters of circRNAs are aberrantly expressed in early-stage lung adenocarcinoma, which might offer potential targets for the early diagnosis of this disease and new genetic insights into lung cancer.

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Section: Resultsmentioning

confidence: 99%

CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients

Zhao

Wang

et al. 2017

Cell Physiol Biochem

105

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“…The lncRNA Cyrano is particularly intriguing because it is broadly conserved in vertebrates and contains a site with unusually high complementarity to the miR-7 miRNA (Ulitsky et al, 2011). Another class of abundant yet enigmatic RNAs is the circular RNAs (circRNAs), which are formed through back-splicing of mRNA or lncRNA exons (Ebbesen et al, 2017). Among the most extensively characterized circRNAs is Cdr1as, which is conserved in mammals and dampens neuronal activity in mice (Hansen et al, 2013; Memczak et al, 2013; Piwecka et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

A Network of Noncoding Regulatory RNAs Acts in the Mammalian Brain

Kleaveland

Shi

Stefano

et al. 2018

Cell

510

538

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SUMMARY Noncoding RNAs (ncRNAs) play increasingly appreciated gene-regulatory roles. Here, we describe a regulatory network centered on four ncRNAs—a long ncRNA, a circular RNA, and two microRNAs—using gene editing in mice to probe the molecular consequences of disrupting key components of this network. The long ncRNA Cyrano uses an extensively paired site to miR-7 to trigger destruction of this microRNA. Cyrano-directed miR-7 degradation is much more effective than previously described examples of target-directed microRNA degradation, which come primarily from studies of artificial and viral RNAs. By reducing miR-7 levels, Cyrano prevents repression of miR-7–targeted mRNAs and enables accumulation of Cdr1as, a circular RNA known to regulate neuronal activity. Without Cyrano, excess miR-7 causes cytoplasmic destruction of Cdr1as in neurons, in part through enhanced slicing of Cdr1as by a second miRNA, miR-671. Thus, several types of ncRNAs can collaborate to establish a sophisticated regulatory network.

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“…These types of transcripts are ubiquitous present in eukaryotic cells and competitively bind microRNAs sequences, functioning like an inhibitory sponge; process that could attribute a significant therapeutic potential to these circular fragments [9,10,11,12]. In this sense, specific microRNAs are eliminated from the regulatory networks, influencing the expression scheme of target genes.…”

Section: Physiologic and Pathologic Angiogenesis -Signaling Mechanismmentioning

confidence: 99%

Noncoding RNAs in Lung Cancer Angiogenesis

Berindan‐Neagoe¹,

Braicu²,

Gulei³

et al. 2017

Physiologic and Pathologic Angiogenesis - Signaling Mechanisms and Targeted Therapy

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Lung cancer is the major death-related cancer in both men and women, due to late diagnostic and limited treatment efficacy. The angiogenic process that is responsible for the support of tumor progression and metastasis represents one of the main hallmarks of cancer. The role of VEGF signaling in angiogenesis is well-established, and we summarize the role of semaphorins and their related receptors or hypoxia-related factors role as prone of tumor microenvironment in angiogenic mechanisms. Newly, noncoding RNA transcripts (ncRNA) were identified to have vital functions in miscellaneous biological processes, including lung cancer angiogenesis. Therefore, due to their capacity to regulate almost all molecular pathways related with altered key genes, including those involved in angiogenesis and its microenvironment, ncRNAs can serve as diagnosis and prognosis markers or therapeutic targets. We intend to summarize the latest progress in the field of ncRNAs in lung cancer and their relation with hypoxia-related factors and angiogenic genes, with a particular focus on ncRNAs relation to semaphorins.

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Insights into circular RNA biology

Cited by 383 publications

References 74 publications

CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients

CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients

A Network of Noncoding Regulatory RNAs Acts in the Mammalian Brain

Noncoding RNAs in Lung Cancer Angiogenesis

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