Insights into centromeric transcription in mitosis

Liu, Hong

doi:10.1080/21541264.2015.1127315

Cited by 9 publications

(9 citation statements)

References 29 publications

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“…As expected, melatonin treatment via oral administration substantially restored the spindle/chromosome structural defects to promote the meiotic progression, suggesting that melatonin indeed has the potential to ameliorate the oocyte quality. Because kinetochore attaches chromosomes to spindle microtubules and maintains the attachment to growing or disassembling microtubules to drive chromosome segregation, spindle/chromosome abnormalities are always accompanied by the errors of kinetochore‐microtubule attachments . We further determined the effect of melatonin on the stability of kinetochore‐microtubule attachments in BPA‐exposed oocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Because kinetochore attaches chromosomes to spindle microtubules and maintains the attachment to growing or disassembling microtubules to drive chromosome segregation, 49,50 spindle/chromosome abnormalities are always accompanied by the errors of kinetochore-microtubule attachments. 17,51,52 We further determined the effect of melatonin on the stability of kinetochore-microtubule attachments in BPA-exposed oocytes. Our findings showed that melatonin significantly elevated proportion of stable kinetochoremicrotubule attachments in BPA-exposed oocytes.…”

Section: Discussionmentioning

confidence: 99%

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Melatonin protects oocyte quality from Bisphenol A‐induced deterioration in the mouse

Zhang

Dai

et al. 2017

Journal of Pineal Research

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Bisphenol A (BPA) has been reported to adversely affect the mammalian reproductive system in both sexes. However, the underlying mechanisms regarding how BPA disrupts the mammalian oocyte quality and how to prevent it have not been fully defined. Here, we document that BPA weakens oocyte quality by impairing both oocyte meiotic maturation and fertilization ability. We find that oral administration of BPA (100 μg/kg body weight per day for 7 days) compromises the first polar body extrusion (78.0% vs 57.0%, P<.05) by disrupting normal spindle assembly, chromosome alignment, and kinetochore-microtubule attachment. This defect could be remarkably ameliorated (76.7%, P<.05) by concurrent oral administration of melatonin (30 mg/kg body weight per day for 7 days). In addition, BPA administration significantly decreases the fertilization rate of oocytes (87.2% vs 41.1%, P<.05) by reducing the number of sperm binding to the zona pellucida, which is consistent with the premature cleavage of ZP2 as well as the mis-localization and decreased protein level of ovastacin. Also, the localization and protein level of Juno, the sperm receptor on the egg membrane, are strikingly impaired in BPA-administered oocytes. Finally, we show that melatonin administration substantially elevates the in vitro fertilization rate (63.0%, P<.05) by restoring above defects of fertilization proteins and events, which might be mediated by the improvement of oocyte quality via reduction of ROS levels and inhibition of apoptosis. Collectively, our data reveal that melatonin has a protective action against BPA-induced deterioration of oocyte quality in mice.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Melatonin protects oocyte quality from Bisphenol A‐induced deterioration in the mouse

Zhang

Dai

et al. 2017

Journal of Pineal Research

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“…congression (46,49). Therefore, spindle and chromosome abnormalities are always accompanied by errors in kinetochore-microtubule attachments (50)(51)(52). We then assessed the stability of kinetochore-microtubule attachments after cool treatment that depolymerizes microtubules that are unattached to kinetochores.…”

Section: Discussionmentioning

confidence: 99%

BaP exposure causes oocyte meiotic arrest and fertilization failure to weaken female fertility

et al. 2017

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Benzo[a]pyrene (BaP) is a ubiquitous environmental pollutant and carcinogen that is frequently found in particulate matter, with a diameter of ≤2.5 μm (PM2.5). It has been reported to interrupt the normal reproductive system, but the exact molecular basis has not been clearly defined. To understand the underlying mechanisms regarding how BaP exposure disrupts female fertility, we evaluated oocyte quality by assessing the critical regulators and events during oocyte meiotic maturation and fertilization. We found that BaP exposure compromised the mouse oocyte meiotic progression by disrupting normal spindle assembly, chromosome alignment, and kinetochore-microtubule attachment, consequently leading to the generation of aneuploid eggs. In addition, BaP administration significantly decreased the fertilization rate of mouse eggs by reducing the number of sperm binding to the zona pellucida, which was consistent with the premature cleavage of N terminus of zona pellucida sperm-binding protein 2 and precocious exocytosis of ovastacin. Furthermore, BaP exposure interfered with the gamete fusion process by perturbing the localization and protein level of Juno. Notably, we found that BaP exposure induced oxidative stress with an increased level of reactive oxygen species and apoptosis in oocytes and thereby led to the deterioration of critical regulators and events during oocyte meiotic progression and fertilization. Our data document that BaP exposure reduces female fertility impairing oocyte maturation and fertilization ability induced by oxidative stress and early apoptosis in murine models.-Zhang, M., Miao, Y., Chen, Q., Cai, M., Dong, W., Dai, X., Lu, Y., Zhou, C., Cui, Z., Xiong, B. BaP exposure causes oocyte meiotic arrest and fertilization failure to weaken female fertility.

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“…This was due to the occurrence of defective spindle assembly and chromosome alignment. Because spindle/chromosome abnormalities are always caused by the error of kinetochore-microtubule attachment [28,29], we further assessed it to show that a large number of free kinetochores which were not attached by the microtubules were present in HU-exposed oocytes, leading to misaligned chromosomes. Therefore, these observations suggest that the nuclear maturation of oocytes is impaired upon HU exposure.…”

Section: Agingmentioning

confidence: 99%

Shoutai pills improve the quality of oocytes exposed to the chemotherapeutic drug Hydroxyurea

Zhang

Tan

Wang

et al. 2020

Aging

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Hydroxyurea (HU), a DNA synthesis inhibitor, is one of the most common chemotherapeutic drugs that have been widely applied to treat a variety of cancers. HU treatment exhibits severe side effects including renal toxicity, skin toxicity and embryo-toxicity. However, the influence of HU on the female gamete development has not yet fully clarified. Here, we found that HU exposure induced the degeneration of activated follicles after primordial follicle stage, resulting in the depletion of the ovarian reserve. HU exposure also led to the oocyte meiotic maturation arrest via disrupting normal spindle assembly, chromosome alignment and kinetochoremicrotubule attachment. Furthermore, exposure to HU impaired the dynamics of ovastacin and Juno, two critical fertilization regulators. Notably, we illustrated that Shoutai pills (STP), a traditional Chinese medicine drug that has been commonly used for the treatment of miscarriage in China, partially restored all of the defects of oocyte development resulting from HU exposure through inhibiting the occurrence of oxidative stress-induced apoptosis. Taken together, our data not only reveal the adverse impact of HU exposure on the female gamete development, but also provide an effective strategy to prevent it, potentially contributing to the improvement of the quality of oocytes from patients treated with HU.

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Insights into centromeric transcription in mitosis

Cited by 9 publications

References 29 publications

Melatonin protects oocyte quality from Bisphenol A‐induced deterioration in the mouse

Melatonin protects oocyte quality from Bisphenol A‐induced deterioration in the mouse

BaP exposure causes oocyte meiotic arrest and fertilization failure to weaken female fertility

Shoutai pills improve the quality of oocytes exposed to the chemotherapeutic drug Hydroxyurea

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