Insights into Cadmium Toxicity: Reactive Oxygen and Nitrogen Species Function

Romero‐Puertas, María C.; Ortega-Galisteo, Ana P.; Rodríguez‐Serrano, María; Sandalio, Luisa M.

doi:10.1007/978-3-642-22081-4_5

Cited by 13 publications

(21 citation statements)

References 148 publications

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“…Oxidative stress has been shown to be one of the main effects of Cd treatment (Romero‐Puertas et al . ). In soybean roots however, a slight but not significant increase in lipid peroxidation used as a marker of membrane damage was observed after 6 h exposure with a subsequent reduction after 72 h until the end of treatment.…”

Section: Discussionmentioning

confidence: 97%

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Cadmium induces two waves of reactive oxygen species in Glycine max (L.) roots

Pérez-Chaca

Rodríguez‐Serrano

Molina

et al. 2014

Plant Cell & Environment

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126

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Cadmium (Cd) is a non-essential heavy metal that may be toxic or even lethal to plants as it can be easily taken up by the roots and loaded into the xylem to the leaves. Using soybean roots (Glycine max L.) DM 4800, we have analysed various parameters related to reactive oxygen metabolism and nitric oxide (NO) during a 6 day Cd exposure. A rise in H2O2 and NO, and to a lesser extent O2 ·− content was observed after 6 h exposure with a concomitant increase in lipid peroxidation and carbonyl group content. Both oxidative markers were significantly reduced after 24 h. A second, higher wave of O2 ·− production was also observed after 72 h of exposure followed by a reduction until the end of the treatment. NOX and glicolate oxidase activity might be involved in the initial Cd-induced reactive oxygen species (ROS) production and it appears that other sources may also participate. The analysis of antioxidative enzymes showed an increase in glutathione-S-transferase activity and in transcript levels and activity of enzymes involved in the ascorbate-glutathione cycle and the NADPH-generating enzymes. These results suggest that soybean is able to respond rapidly to oxidative stress imposed by Cd by improving the availability of NADPH necessary for the ascorbate-glutathione cycle.

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Section: Discussionmentioning

confidence: 97%

“…In particular, production of NO in response to Cd has been described and it appears that it is dose‐ and time‐dependent (Romero‐Puertas et al . ).…”

Section: Introductionmentioning

confidence: 97%

Cadmium induces two waves of reactive oxygen species in Glycine max (L.) roots

Pérez-Chaca

Rodríguez‐Serrano

Molina

et al. 2014

Plant Cell & Environment

Self Cite

126

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“…H 2 O 2 production after 15 min of Cd-treatment has been reported in Nicotiana tabacum cell suspensions (Olmos et al, 2003) and after 1 h in Oryza sativa (Yeh et al, 2007). Different studies on both whole plant (Di Baccio et al, 2014) or detached leaves (Hsu and Kao, 2007;Iannone et al, 2010) level, demonstrate that Cd challenge elicits reactive oxygen species (ROS) production and H 2 O 2 accumulation in cell wall, plasmamembrane, tonoplast, chloroplasts and mitochondria (Zhang et al, 2009;Romero-Puertas et al, 2012). Moreover, it has also been reported that guard cells represent an important gate for Cd entry in leaves and that their cell wall functions as a primary site of Cd accumulation and of H 2 O 2 production (Olmos et al, 2003;Hsu and Kao, 2007;Rodríguez-Serrano et al, 2009).…”

Section: Discussionmentioning

confidence: 98%

Effect of short-term cadmium stress on Populus nigra L. detached leaves

Lomaglio

Rocco

Trupiano

et al. 2015

Journal of Plant Physiology

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“…It has been well documented that Cd stress will induce ROS production MDA accumulation (Romero-Puertas et al, 2012; Sandalio et al, 2012). In the present study, the increase of

O_{2}^{• -}

and H 2 O 2 production in apple rootstocks after Cd exposure reflected the oxidative stress induced by Cd.…”

Section: Discussionmentioning

confidence: 99%

Integration of Cadmium Accumulation, Subcellular Distribution, and Physiological Responses to Understand Cadmium Tolerance in Apple Rootstocks

Zhou

Wan

et al. 2017

Front. Plant Sci.

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Cadmium (Cd) is a nonessential and highly toxic element causing agricultural problems. However, little information is available about the variation in Cd tolerance among apple rootstocks and its underlying physiological regulation mechanisms. This study investigated Cd accumulation, subcellular distribution, and chemical forms as well as physiological changes among four apple rootstocks exposed to either 0 or 300 μM CdCl2. The results showed that variations in Cd tolerance existed among these rootstocks. Cd exposure caused decline in photosynthesis, chlorophyll and biomass in four apple rootstocks, which was less pronounced in M. baccata, indicating its higher Cd tolerance. This finding was corroborated with higher Cd tolerance indexes (TIs) of the whole plant in M. baccata than those in the other three apple rootstocks. Among the four apple rootstocks, M. baccata displayed the lowest Cd concentrations in roots, wood, and leaves, the smallest total Cd amounts as well as the lowest BCF. In apple rootstocks, it was found that to immobilize Cd in cell wall and soluble fraction (most likely in vacuole) and to convert it into pectate- or protein- integrated forms and undissolved Cd phosphate forms may be the primary strategies to reduce Cd mobility and toxicity. The physiological changes including ROS, carbohydrates and antioxidants were in line with the variations of Cd tolerance among four apple rootstocks. In comparison with the other three apple rootstocks, M. baccata had lower concentrations of ROS in roots and bark, H2O2 in roots and leaves and MDA in roots, wood and bark, but higher concentrations of soluble sugars in bark and starch in roots and leaves, and enhanced antioxidants. These results indicate that M. baccata are more tolerant to Cd stress than the other three apple rootstocks under the current experiment conditions, which is probably related to Cd accumulation, subcellular partitioning and chemical forms of Cd and well-coordinated antioxidant defense mechanisms.

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Insights into Cadmium Toxicity: Reactive Oxygen and Nitrogen Species Function

Cited by 13 publications

References 148 publications

Cadmium induces two waves of reactive oxygen species in Glycine max (L.) roots

Cadmium induces two waves of reactive oxygen species in Glycine max (L.) roots

Effect of short-term cadmium stress on Populus nigra L. detached leaves

Integration of Cadmium Accumulation, Subcellular Distribution, and Physiological Responses to Understand Cadmium Tolerance in Apple Rootstocks

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