Insights into aureocin A70 regulation: participation of regulator AurR, alternative transcription factor σB and phage ϕ11 regulator cI

Coelho, Marcus Lívio Varella; Fleming, Luana Rocha; Bastos, Maria do Carmo de Freire

doi:10.1016/j.resmic.2015.10.004

Cited by 10 publications

(9 citation statements)

References 40 publications

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“…The comparative study of the gene expression of the Δ bac mutant strain vs. WT strain underpinned the role of EntDD14 in the positive regulation of the ddCDEFGHIJ genes since all of them were significantly down-regulated in the Δ bac mutant strain, mainly after 24 h of growth ( Figure 7 , Table S1 ). Recent studies have reported the implication of transcriptional regulators in the synthesis of lacticin Q and aureocin A70 (Coelho et al, 2016 ; Iwatani et al, 2016 ). Environmental factors such as temperature or nutrition-adaptation were reported to control biosynthesis of enterocin L50 and enterocin Q in the multiple bacteriocin producing strains Ent.…”

Section: Discussionmentioning

confidence: 99%

A Leaderless Two-Peptide Bacteriocin, Enterocin DD14, Is Involved in Its Own Self-Immunity: Evidence and Insights

Ladjouzi

Lucau-Danila

Benachour

et al. 2020

Front. Bioeng. Biotechnol.

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Enterocin DD14 (EntDD14) is a two-peptide leaderless bacteriocin produced by Enterococcus faecalis 14, a strain previously isolated from meconium. EntDD14 has a strong antibacterial activity against Gram-positive bacteria. Leaderless bacteriocins, unlike bacteriocins with leader peptides, are immediately active after their translation, and a producing strain has then to develop specific mechanisms to protect both intra and extracellular compartments. The in silico analysis of Ent. faecalis 14 genome allowed to locate downstream of structural ddAB genes, 8 other adjacent genes, designed ddCDEFGHIJ, which collectively may form three operons. To gain insights on immunity mechanisms of Ent. faecalis 14, mutant strains knocked out in ddAB genes encoding bacteriocin precursor peptides (bac) and/or ABC transporter (ddI) of EntDD14 were constructed and characterized. Importantly, bac mutant strains, from which structural ddAB genes were deleted, resulted unable to produce EntDD14 and sensitive to exogenous EntDD14 showing their involvement in the Ent. faecalis 14 immunity system. Moreover, the sensitivity of bac mutants appeared not to be associated with the down-regulation of ddCDEFGHIJ gene expression since they were similarly expressed in both bac and wild-type strains during the log phase while they were found significantly down-regulated in the bac mutant strain after 24 h of growth. Data gathered from this study suggest also the implication of the ABC transporter (ddHIJ) in the active export of EntDD14 but ruled-out its involvement in the primary self-immunity system. Interestingly, non-bacteriocin producing Ent. faecalis JH2-2 cells transformed with ddAB, or ddAB plus genes encoding the ABC transporter (ddAB-HIJ) did not produce EntDD14 and remained sensitive to its action. Of note, trans-complementation of the bac mutant strain with these constructions allowed to recover the WT phenotype. To the best of our knowledge, this is the first study delineating the role of the intracellular two-peptide leaderless bacteriocins in their self-immunity.

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Section: Discussionmentioning

confidence: 99%

A Leaderless Two-Peptide Bacteriocin, Enterocin DD14, Is Involved in Its Own Self-Immunity: Evidence and Insights

Ladjouzi

Lucau-Danila

Benachour

et al. 2020

Front. Bioeng. Biotechnol.

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“…This suggests that the CI protein may play a role within the bacterial regulatory networks. Interestingly, the regulator CI from phage ϕ11 was shown to inhibit aureocin A79 production in synergy with the bacterial regulatory protein AurR 52 . Nonetheless, further research is still necessary to determine how these interactions take place.…”

Section: Discussionmentioning

confidence: 99%

Lysogenization of Staphylococcus aureus RN450 by phages ϕ11 and ϕ80α leads to the activation of the SigB regulon

Fernández

González

Quiles-Puchalt

et al. 2018

Sci Rep

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Staphylococcus aureus is a major opportunistic pathogen that commonly forms biofilms on various biotic and abiotic surfaces. Also, most isolates are known to carry prophages in their genomes. With this in mind, it seems that acquiring a better knowledge of the impact of prophages on the physiology of S. aureus biofilm cells would be useful for developing strategies to eliminate this pathogen. Here, we performed RNA-seq analysis of biofilm cells formed by S. aureus RN450 and two derived strains carrying prophages ϕ11 and ϕ80α. The lysogenic strains displayed increased biofilm formation and production of the carotenoid pigment staphyloxanthin. These phenotypes could be partly explained by the differences in gene expression displayed by prophage-harboring strains, namely an activation of the alternative sigma factor (SigB) regulon and downregulation of genes controlled by the Agr quorum-sensing system, especially the decreased transcription of genes encoding dispersion factors like proteases. Nonetheless, spontaneous lysis of part of the population could also contribute to the increased attached biomass. Interestingly, it appears that the phage CI protein plays a role in orchestrating these phage-host interactions, although more research is needed to confirm this possibility. Likewise, future studies should examine the impact of these two prophages during the infection.

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“…In the case of lacticin Q, it was recently shown that LnqR, a protein belonging to the TetR family of transcriptional regulators, positively regulates the transcription of its respective biosynthetic genes lnqBCDEF and consequently its production ( Iwatani et al, 2016 ). While in the case of aureocin A70, a complex mechanism involving a protein with a helix-turn-helix (HTH) AurR, an alternative transcription factor σ B , and a phage regulator ϕ11, regulates its production ( Coelho et al, 2016 ). Whereas in the case of the W. hellenica QU 13, the producer of weissellicins Y and M, it was demonstrated that its bacteriocin production has a nutrition-adaptive control.…”

Section: Leaderless Bacteriocinsmentioning

confidence: 99%

Circular and Leaderless Bacteriocins: Biosynthesis, Mode of Action, Applications, and Prospects

2018

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Bacteriocins are a huge family of ribosomally synthesized peptides known to exhibit a range of bioactivities, most predominantly antibacterial activities. Bacteriocins from lactic acid bacteria are of particular interest due to the latter’s association to food fermentation and the general notion of them to be safe. Among the family of bacteriocins, the groups known as circular bacteriocins and leaderless bacteriocins are gaining more attention due to their enormous potential for industrial application. Circular bacteriocins and leaderless bacteriocins, arguably the least understood groups of bacteriocins, possess distinctively peculiar characteristics in their structures and biosynthetic mechanisms. Circular bacteriocins have N-to-C- terminal covalent linkage forming a structurally distinct circular peptide backbone. The circular nature of their structures provides them superior stability against various stresses compared to most linear bacteriocins. The molecular mechanism of their biosynthesis, albeit has remained poorly understood, is believed to possesses huge application prospect as it can serve as scaffold in bioengineering other biologically important peptides. On the other hand, while most bacteriocins are synthesized as inactive precursor peptides, which possess an N-terminal leader peptide attached to a C-terminal propeptide, leaderless bacteriocins are atypical as they do not have an N-terminal leader peptide, hence the name. Leaderless bacteriocins are active right after translation as they do not undergo any post-translational processing common to other groups of bacteriocins. This “simplicity” in the biosynthesis of leaderless bacteriocins offers a huge commercial potential as scale-up production systems are considerably easier to assemble. In this review, we summarize the current studies of both circular and leaderless bacteriocins, highlighting the progress in understanding their biosynthesis, mode of action, application and their prospects.

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Insights into aureocin A70 regulation: participation of regulator AurR, alternative transcription factor σB and phage ϕ11 regulator cI

Cited by 10 publications

References 40 publications

A Leaderless Two-Peptide Bacteriocin, Enterocin DD14, Is Involved in Its Own Self-Immunity: Evidence and Insights

A Leaderless Two-Peptide Bacteriocin, Enterocin DD14, Is Involved in Its Own Self-Immunity: Evidence and Insights

Lysogenization of Staphylococcus aureus RN450 by phages ϕ11 and ϕ80α leads to the activation of the SigB regulon

Circular and Leaderless Bacteriocins: Biosynthesis, Mode of Action, Applications, and Prospects

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