Insights from lncRNAs Profiling of MIN6 Beta Cells Undergoing Inflammation

Sun, Chuntao; Xue, Le; Zhu, Zhaohui; Zhang, Fan; Yang, Ronghua; Yuan, Xuewen; Liu, Qianqi

doi:10.1155/2016/9275106

Cited by 18 publications

(14 citation statements)

References 44 publications

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“…Non-coding RNAs have been detected in different tissues or organs suggesting that they may be potential biomarkers for sepsis and sepsis-related organ dysfunction ( Supplementary Tables 1 [ 79 - 83 , 97 - 100 ] and 2 [ 18 , 21 , 22 , 73 , 85 , 101 - 120 ]). Nowadays, the clinical sample which is suitable for detect non-coding RNAs is blood from patients, including serum, plasma, and blood leucocytes.…”

Section: Non-coding Rnas As Potential Biomarkers In Sepsismentioning

confidence: 99%

Non-coding RNA: a potential biomarker and therapeutic target for sepsis

Zhang

Xia

et al. 2017

Oncotarget

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Sepsis, a syndrome of physiologic, pathologic, and biochemical abnormalities caused by an altered systemic host response to infection, has become the main cause of death among patients admitted to the intensive care units. Recently, genome-wide expression analysis revealed that over 80% of the essential genetic elements were altered in critically ill patients. Notably, non-coding RNAs, including microRNAs, long non-coding RNAs and circular RNAs, have been proven to play essential roles in innate immunity, mitochondrial dysfunction and organ dysfunction. In this review, we introduced the biogenesis of non-coding RNAs briefly and summed up different kinds of non-coding RNAs in regulation of sepsis, which could provide a more comprehensive understanding about pathogenesis of the disease. Additionally, we summarized the limitations of current biomarkers and then recommended some non-coding RNAs as novel potential biomarkers for sepsis and sepsis-induced organ dysfunction. Besides, we also introduced some problems and challenges that need to be overcome during the clinical application of non-coding RNAs. Future research should focus on elucidating their molecular mechanisms, particularly long non-coding RNAs as well as circular RNAs and sepsis, to further understanding of the disease process. With the in-depth understanding of the mechanism of sepsis, non-coding RNAs provide a new insight into sepsis and could become the novel therapeutic targets in the future.

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Section: Non-coding Rnas As Potential Biomarkers In Sepsismentioning

confidence: 99%

Non-coding RNA: a potential biomarker and therapeutic target for sepsis

Zhang

Xia

et al. 2017

Oncotarget

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“…T1DM is an organ-specific autoimmune disease characterized by chronic and progressive apoptotic destruction of pancreatic β cells. Research in recent years indicates that LncRNAs modulate the apoptosis of sensitive β cell and they may emerge as novel biomarkers in diagnosis and as targets for development of new therapies for T1DM [ 84 , 163 ].…”

Section: Introductionmentioning

confidence: 99%

LncRNAs: key players and novel insights into diabetes mellitus

Xiao

et al. 2017

Oncotarget

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Long non-coding RNAs (LncRNAs) are a class of endogenous RNA molecules, which have a transcribing length of over 200 nt, lack a complete functional open reading frame (ORF), and rarely encode a functional short peptide. Recent studies have revealed that disruption of LncRNAs levels correlates with several human diseases, including diabetes mellitus (DM), a complex multifactorial metabolic disorder affecting more than 400 million people worldwide. LncRNAs are emerging as pivotal regulators in various biological processes, in the progression of DM and its associated complications, involving pancreatic β-cell disorder, insulin resistance, and epigenetic regulation, etc. Further investigation into the mechanisms of action of LncRNAs in DM will be of great value in the thorough understanding of pathogenesis. However, prior to successful application of LncRNAs, further search for molecular biomarkers and drug targets to provide a new strategy for DM prevention, early diagnosis, and therapy is warranted.

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“…Total RNA was isolated from the spleen using TRIzol reagent (Invitrogen, Life Technologies Corp., CA, USA), and cDNA was synthesized using a Revert Aid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Waltham, MA, USA) for qRT-PCR using a ChamQTM SYBR ® qPCR Master Mix (Vazyme, Biotech, Nanjing, China) and primers specific for TLR4 (forward 5′-ATGGCATGGCTTACACCACC-3′ and reverse 5′-GAGGCCAATTTTGTCTCCACA-3′) [ 12 ] and β-actin (forward 5′-AGAGGGAAATCGTGCGTGAC-3′ and reverse 5′-CAATAGTGATGACCTGGCCGT-3′) [ 32 ]. The reaction conditions were as follows: pre-denaturation at 95°C for 30 sec, followed by 40 cycles of denaturation at 95°C for 10 sec and annealing and extension at 60°C for 30 sec.…”

Section: Methodsmentioning

confidence: 99%

Poplar bark lipids enhance mouse immunity by inducing T cell proliferation and differentiation

Tang

Wei

et al. 2020

J. Vet. Med. Sci.

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Research on the composition and application of immune enhancers in livestock and poultry breeding has been gaining interest in recent years. Poplar bark lipids (PBLs), which are extracted from poplar tree bark, are natural substances known to efficiently enhance the immune response. To understand the chemical makeup of PBLs and their underlying mechanism for enhancing the immune system, we extracted PBLs from poplar bark using petroleum ether and subjected these extracts to chemical analysis. To evaluate PBLs effect on the immune system mice were treated with different doses of PBL via gavage and sacrificed 4 weeks later. PBLs were shown to be rich in vitamin E, unsaturated fatty acids, and other immune-potentiating compounds. Treatment with PBLs increased the spleen index and stimulated spleen and thymus development. In addition, PBLs increased the number of CD3 + CD4 + cells in the peripheral blood and the ratio of CD4 + /CD8 + cells while decreasing the number of CD3 + CD8 + cells. Moreover, PBLs significantly increased IL-4 and IFN-γ levels in mouse serum and TLR4 mRNA and protein expression in the spleen. Taken together these results demonstrate that PBLs exert their immune-potentiating effects by promoting spleen and thymus development, T lymphocyte proliferation and differentiation, and immune factor expression. These immune-potentiating effects may be related to the activation of TLR4. This study provides a theoretical basis for the development of PBLs as an immune adjuvant or feed additive in the future.

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Insights from lncRNAs Profiling of MIN6 Beta Cells Undergoing Inflammation

Cited by 18 publications

References 44 publications

Non-coding RNA: a potential biomarker and therapeutic target for sepsis

Non-coding RNA: a potential biomarker and therapeutic target for sepsis

LncRNAs: key players and novel insights into diabetes mellitus

Poplar bark lipids enhance mouse immunity by inducing T cell proliferation and differentiation

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