Insight on esterase from Pseudomonas aeruginosa strain S3 that depolymerize poly(lactic acid) (PLA) at ambient temperature

Noor, Hazrat; Satti, Sadia Mehmood; Din, Salah Ud; Farman, Muhammad; Hasan, Fariha; Khan, Samiullah; Badshah, Malik; Shah, Aamer Ali

doi:10.1016/j.polymdegradstab.2020.109096

Cited by 27 publications

(19 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Geobacillus is very well reported in the literature for biofilm and spore formation [95,96]. Anthranilate phosphoribosyltransferase (EC 2.4.2.18) [97] and 5 -nucleotidase (EC 3.1.3.5), reported to have an impact on virulence and biofilm formation, were found in our Geobacil-lus sp. EC-3 [98].…”

Section: Genetic Determinants For Biofilm Formation and Regulationsupporting

confidence: 78%

See 1 more Smart Citation

Genome Annotation of Poly(lactic acid) Degrading Pseudomonas aeruginosa, Sphingobacterium sp. and Geobacillus sp.

Satti

Castro‐Aguirre

Shah

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Pseudomonas aeruginosa and Sphingobacterium sp. are well known for their ability to decontaminate many environmental pollutants while Geobacillus sp. have been exploited for their thermostable enzymes. This study reports the annotation of genomes of P. aeruginosa S3, Sphingobacterium S2 and Geobacillus EC-3 that were isolated from compost, based on their ability to degrade poly(lactic acid), PLA. Draft genomes of the strains were assembled from Illumina reads, annotated and viewed with the aim of gaining insight into the genetic elements involved in degradation of PLA. The draft genome of Sphinogobacterium strain S2 (435 contigs) was estimated at 5,604,691 bp and the draft genome of P. aeruginosa strain S3 (303 contigs) was estimated at 6,631,638 bp. The draft genome of the thermophile Geobacillus strain EC-3 (111 contigs) was estimated at 3,397,712 bp. A total of 5385 (60% with annotation), 6437 (80% with annotation) and 3790 (74% with annotation) protein-coding genes were predicted for strains S2, S3 and EC-3, respectively. Catabolic genes for the biodegradation of xenobiotics, aromatic compounds and lactic acid as well as the genes attributable to the establishment and regulation of biofilm were identified in all three draft genomes. Our results reveal essential genetic elements that facilitate PLA metabolism at mesophilic and thermophilic temperatures in these three isolates.

show abstract

Section: Genetic Determinants For Biofilm Formation and Regulationsupporting

confidence: 78%

“…In our previous studies we have reported the expression of PLA hydrolyzing lipase and esterase from our isolates P. aeruginosa strain S3 and Sphingobacterium sp. strain S2 [12,97,98]. Genome annotation of S2, S3 and EC-3 reveal the presence of numerous hydrolytic enzymes (Table 5).…”

Section: Enzymesmentioning

confidence: 99%

Genome Annotation of Poly(lactic acid) Degrading Pseudomonas aeruginosa, Sphingobacterium sp. and Geobacillus sp.

Satti

Castro‐Aguirre

Shah

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, an esterase from Pseudomonas aeruginosa strain S3 capable of depolymerizing PLA films was purified with a molecular mass of approximately 34 kDa. This enzyme was stable at wide temperature and pH ranges, with highest stability at 30°C and pH 7.0 (Noor et al, 2020).…”

Section: Mesostable and Cold Active/adapted Pla Depolymerasesmentioning

confidence: 97%

Microbial Degradation of Polylactic Acid Bioplastic

Leow¹,

Rahman²

2021

JSSM

View full text Add to dashboard Cite

“…An esterase gene was also cloned from AHK119 and the recombinant enzyme on purification was reported as a thermoactive esterase (Hu et al 2010). Several researchers have reported esterases from bacterial strain with substrate specificity against wide variety of polyester‐based BPs (Shah et al 2013b; Hajighasemi et al 2016; Noor et al 2020).…”

Section: Role Of Enzymes In Biodegradationmentioning

confidence: 99%

Polyester‐based biodegradable plastics: an approach towards sustainable development

Satti

Shah

2020

Lett Appl Microbiol

View full text Add to dashboard Cite

Significance and Impact of the Study: Polyester-based BPs considered as a solution to current plastic waste problem as well as leading polymers in terms of biodegradability and sustainability has been critically discussed. The role of microorganisms and their enzymes involved in the biodegradation of these polymers and ecotoxicological impact of degradation products of BPs on soil microbial community and biogeochemical cycles has also been described. This report will provide an insight on the key research areas to bridge the gap for development of simulated systems as an effective and emerging strategy to divert the overflow of plastic in the environment as well as for the greener solution to the plastic waste management problems. AbstractNon-degradability of conventional plastics, filling of landfill sites, raising water and land pollution and rapid depletion of fossil resources have raised the environmental issues and global concerns. The current demand and production of plastics is putting immense pressure on fossil resources, consuming about 6% of the global oil and is expected to grow up to 20%. The polyester-based biodegradable plastics (BPs) are considered as a remedy to the issue of plastics waste in the environment. BPs appear to manage the overflow of plastics by providing new means of waste management system and help in securing the non-renewable resources of nature. This review comprehensively presents the environmental burdens due to conventional plastics as well as production of polyester-based BPs as an alternative to conventional commodity plastics. The diversity of micro-organisms and their enzymes that degrade various polyesterbased BPs (PLA, PCL, PHB/PHBV and PET) has also been described in detail. Moreover, the impact of plastics degradation products on soil ecology and ecosystem functions has critically been discussed. The report ends with special focus on future recommendations for the development of sustainable waste management strategies to control pollution due to plastics waste. Letters in Applied Microbiology ISSN 0266-8254Packaging, compost bags and agricultural mulching films Li et al. (2015) Letters in Applied Microbiology 70, 413--430

show abstract

Insight on esterase from Pseudomonas aeruginosa strain S3 that depolymerize poly(lactic acid) (PLA) at ambient temperature

Cited by 27 publications

References 38 publications

Genome Annotation of Poly(lactic acid) Degrading Pseudomonas aeruginosa, Sphingobacterium sp. and Geobacillus sp.

Genome Annotation of Poly(lactic acid) Degrading Pseudomonas aeruginosa, Sphingobacterium sp. and Geobacillus sp.

Microbial Degradation of Polylactic Acid Bioplastic

Polyester‐based biodegradable plastics: an approach towards sustainable development

Contact Info

Product

Resources

About