Insight into the Sensing Behavior of DNA Probes Based on MOF–Nucleic Acid Interaction for Bioanalysis

Abstract

Adsorption of DNA probes onto nanomaterials is a promising strategy for bioassay establishment typically using fluorescence or catalytic activities to generate signals. Albeit important, there is currently a lack of systematic understanding of the sensing behaviors building on nanomaterial–DNA interactions, which greatly limits the rational method design and their subsequent applications. Herein, the issue was investigated by employing multifunctional metal–organic frameworks (MOFs) (FeTCPP⊂UiO-66) as a model that was synthesized via integrating heme-like ligand FeTCPP into commonly used MOFs (UiO-66). Our results demonstrated that the fluorescently labeled DNA adsorbed onto FeTCPP⊂UiO-66 was quenched through photoinduced electron transfer, fluorescence resonance energy transfer, and the internal filtration effect. Among different DNA structures, double-stranded DNA and hybridization chain reaction products largely retained their fluorescence due to desorption and conformational variation, respectively. In addition, ssDNA could maximally inhibit the peroxidase activity of FeTCPP⊂UiO-66, and this inhibition was strongly dependent on the strand length but independent of base composition. On the basis of these discoveries, a fluorescence/colorimetric dual-modal detection was designed against aflatoxin B1 with satisfactory performances obtained to further verify our results. This study provided some new insights into the sensing behaviors based on MOF–DNA interactions, indicating promising applications for rational bioassay design and its performance improvement.

Morphology Control of Zr-Based Luminescent Metal-Organic Frameworks for Aflatoxin B1 Detection

“…The C 1s spectrum ( Figure 1 D) exhibits peaks at 284.8 and 288.8 eV, assigned to C=C and −COOH in TCPB 4− , respectively. The Zr 3d spectrum ( Figure S2 ) displays distinct peaks for Zr 3d 5/2 and Zr 3d 3/2 at 182.6 and 185.0 eV [ 32 , 33 ], respectively, demonstrating the existence of Zr 4+ . All the results affirm the successful synthesis of ellipsoid-like Zr-LMOF.…”

Tuning atomic-scale sites in metal–organic framework-based nanozymes for sensitive biosensing

“…10, to achieve sensitive detection of toxins, Zhang's group designed a dual-modal fluorescence/colorimetric detection method for detecting aflatoxin B1 (AFB1) by combining DNA probes with MOFs. 99 First, a heme-like ligand FeTCPP was integrated into UiO-66 to generate FeTCPP⊂UiO-66, which endowed the material with POD-like activity and enhanced its fluorescence quenching ability. After AFB1 is captured by the aptamer, blocking DNA will be released from magnetic beads (MBs), and then MBs are removed by magnetic adsorption, only blocking DNA is left.…”

“On–On–Off” Recyclable Fluorescence Battery for Direct and Selective Detection of Glyphosate and Cu2+

“…Nowadays, fluorescence nanomaterials, such as quantum dots (QDs), covalent organic frameworks, and metal–organic frameworks (MOFs), are receiving considerable attention and are widely used to develop fluorescence sensors, owing to the surface plentiful active sites, high biocompatibility, and excellent structure stability. , MOFs have excellent advantages, such as ease of synthesis, adjustable optical properties, excellent stability, and low cost . Various MOF nanomaterials have been successfully utilized in different fields, such as food safety, , environmental protection, , and early cancer diagnosis. − For this reason, developing OP detection sensors with MOF nanomaterials is prospective. Some groups have used functioned MOFs to detect OPs; for example, Li et al designed a chemiluminescence sensor to detect glyphosate (Gly) via an alkali solution treating UIO-66 surface to form a hydroxy zirconium oxide nanozyme (ZrOX-OH) .…”