Insight into the Possible Use of the Predator Bdellovibrio bacteriovorus as a Probiotic

Bonfiglio, Giulia; Neroni, Bruna; Radocchia, Giulia; Migliori, Massimiliano; Pantanella, Fabrizio; Schippa, Serena

doi:10.3390/nu12082252

Cited by 25 publications

(15 citation statements)

References 139 publications

(223 reference statements)

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“…The use of BALOs as biological control agents in environmental and medical microbiological settings 18 , 31 has been suggested based on their lack of interaction with human cells 32 – 34 . As occurs with antibiotics, testing the individual in vitro susceptibility for prey and predator pairs of strains is a requirement, mainly when predation could be substantially affected by environmental or biological conditions, as we postulate herein.…”

Section: Discussionmentioning

confidence: 99%

Strain-specific predation of Bdellovibrio bacteriovorus on Pseudomonas aeruginosa with a higher range for cystic fibrosis than for bacteremia isolates

Saralegui

Herencias

Halperin

et al. 2022

Sci Rep

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This work aimed to evaluate the predatory activity of Bdellovibrio bacteriovorus 109J on clinical isolates of Pseudomonas aeruginosa selected from well-characterized collections of cystic fibrosis (CF) lung colonization (n = 30) and bloodstream infections (BSI) (n = 48) including strains selected by genetic lineage (frequent and rare sequence types), antibiotic resistance phenotype (susceptible and multidrug-resistant isolates), and colony phenotype (mucoid and non-mucoid isolates). The intraspecies predation range (I-PR) was defined as the proportion of susceptible strains within the entire collection. In contrast, the predation efficiency (PE) is the ratio of viable prey cells remaining after predation compared to the initial inoculum. I-PR was significantly higher for CF (67%) than for BSI P. aeruginosa isolates (35%) probably related to an environmental origin of CF strains whereas invasive strains are more adapted to humans. I-PR correlation with bacterial features such as mucoid morphotype, genetic background, or antibiotic susceptibility profile was not detected. To test the possibility of increasing I-PR of BSI isolates, a polyhydroxyalkanoate depolymerase deficient B. bacteriovorus bd2637 mutant was used. Global median I-PR and PE values remained constant for both predators, but 31.2% of 109J-resistant isolates were susceptible to the mutant, and 22.9% of 109J-susceptible isolates showed resistance to predation by the mutant, pointing to a predator–prey specificity process. The potential use of predators in the clinical setting should be based on the determination of the I-PR for each species, and the PE of each particular target strain.

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Section: Discussionmentioning

confidence: 99%

Strain-specific predation of Bdellovibrio bacteriovorus on Pseudomonas aeruginosa with a higher range for cystic fibrosis than for bacteremia isolates

Saralegui

Herencias

Halperin

et al. 2022

Sci Rep

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“…This may be explained by the detection of DNA fragments resulting from bacterial lysis. Bdellovibrionaceae has been found in soil, freshwater and the human gut from both healthy subjects and those suffering from inflammatory diseases 46 , 47 . This taxon is considered as a potential probiotic, since it could modulate gut biodiversity through predation of bacteria correlated in chronic inflammatory diseases, such as obesity and Crohn’s disease 48 , 49 .…”

Section: Discussionmentioning

confidence: 99%

Differential analysis of the bacterial community in colostrum samples from women with gestational diabetes mellitus and obesity

Gámez-Valdez

García-Mazcorro²,

Montoya-Rincón

et al. 2021

Sci Rep

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Gestational Diabetes Mellitus (GDM) and obesity affect the functioning of multiple maternal systems and influence colonization of the newborn gastrointestinal through the breastmilk microbiota (BMM). It is currently unclear how GDM and obesity affect the human BMM composition. Here, we applied 16S-rRNA high-throughput sequencing to human colostrum milk to characterize BMM taxonomic changes in a cohort of 43 individuals classified in six subgroups according to mothers patho-physiological conditions (healthy control (n = 18), GDM (n = 13), or obesity (n = 12)) and newborn gender. Using various diversity indicators, including Shannon/Faith phylogenetic index and UniFrac/robust Aitchison distances, we evidenced that BMM composition was influenced by the infant gender in the obesity subgroup. In addition, the GDM group presented higher microbial diversity compared to the control group. Staphylococcus, Corynebacterium 1, Anaerococcus and Prevotella were overrepresented in colostrum from women with either obesity or GDM, compared to control samples. Finally, Rhodobacteraceae was distinct for GDM and 5 families (Bdellovibrionaceae, Halomonadaceae, Shewanellaceae, Saccharimonadales and Vibrionaceae) were distinct for obesity subgroups with an absolute effect size greater than 1 and a q-value ≤ 0.05. This study represents the first effort to describe the impact of maternal GDM and obesity on BMM.

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“…The use of BALOs as biological control agents in environmental and medical microbiological settings (22,44) has been suggested based on their lack of interaction with human cells (45).…”

Section: Discussionmentioning

confidence: 99%

Predation Efficiency upon Clinical Isolates: Bdellovibrio bacteriovorus is Prey Specific and Origin Dependent

Herencias

Saralegui

Halperin

et al. 2021

Preprint

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The use of predatory bacteria as live antibiotics has been proposed for managing bacterial infections, especially for those caused by antibiotic multiresistant isolates for which there are few therapeutic options. However, the current knowledge in this field is scarce, with most of the available data based on environmental isolates, with a significant lack of human clinical samples. Here, we evaluated the predatory spectrum of the reference strain Bdellovibrio bacteriovorus 109J on 13 Serratia marcescens (including carbapenemase producers) and 78 Pseudomonas aeruginosa clinical isolates from respiratory (colonizing lungs of cystic fibrosis patients) or bacteremic infections, differentiated by phenotype (mucoid or not), antibiotic resistance phenotype (including multidrug-resistant isolates), and genetic lineage (frequent and rare sequence types). The source of the isolates was significantly associated with predation efficiency (100% for S. marcescens, 67% for P. aeruginosa from cystic fibrosis, and 25% for P. aeruginosa from bacteremia). In contrast, no correlation with colonial morphotype, genetic background, or antibiotic susceptibility was found. To evaluate the influence of the predator on the predation, we employed a more aggressive B. bacteriovorus mutant 109J preying upon the same 48 bacteremic P. aeruginosa isolates. The mutant's predation efficiency was higher than that of their wild-type counterpart (43% vs. 25%), pointing out that predation is specific to each prey-predator pair. Our results provide the most extensive study of clinical prey susceptibility published to date and show that the prey-predator interaction is influenced by the origin of the isolates rather than by their genetic background or their antibiotic susceptibility phenotype.

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Insight into the Possible Use of the Predator Bdellovibrio bacteriovorus as a Probiotic

Cited by 25 publications

References 139 publications

Strain-specific predation of Bdellovibrio bacteriovorus on Pseudomonas aeruginosa with a higher range for cystic fibrosis than for bacteremia isolates

Strain-specific predation of Bdellovibrio bacteriovorus on Pseudomonas aeruginosa with a higher range for cystic fibrosis than for bacteremia isolates

Differential analysis of the bacterial community in colostrum samples from women with gestational diabetes mellitus and obesity

Predation Efficiency upon Clinical Isolates: Bdellovibrio bacteriovorus is Prey Specific and Origin Dependent

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