2019
DOI: 10.1016/j.freeradbiomed.2019.06.003
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Insight into the cellular effects of nitrated phospholipids: Evidence for pleiotropic mechanisms of action

Abstract: Nitrated phospholipids have been recently identified in biological systems and showed to display anti-oxidant and anti-inflammatory potential in models of inflammation in vitro. Here, we have explored the effects of nitrated 1-palmitoyl-2-oleyl-phosphatidyl choline (NO 2-POPC) in cellular models. We have observed that NO 2-POPC, but not POPC, induces cellular changes consisting in cytoskeletal rearrangement and cell shrinking, and ultimately, loss of cell adhesion or impaired cell attachment. NO 2-POPC release… Show more

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Cited by 13 publications
(20 citation statements)
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“…These modified lipids showed antioxidant properties thanks to their radical scavenging potential against the 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical cation (ABTS •+ ) and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH • ) [11,32] and can also act as anti-inflammatory agents by inhibiting the expression of inducible nitric oxide synthase (iNOS; at the protein level) in RAW 264.7 macrophages treated with lipopolysaccharide [32]. Moreover, nitrated POPC induced a series of downstream cellular effects in SW13/cl.2 cells, showing nitrated PLs as new potential electrophilic lipid mediators with selective actions [33]. However, their structural characteristics can have a great impact on their biological significance, as previously reported for NO 2 -FAs (reviewed in [7]).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…These modified lipids showed antioxidant properties thanks to their radical scavenging potential against the 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical cation (ABTS •+ ) and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH • ) [11,32] and can also act as anti-inflammatory agents by inhibiting the expression of inducible nitric oxide synthase (iNOS; at the protein level) in RAW 264.7 macrophages treated with lipopolysaccharide [32]. Moreover, nitrated POPC induced a series of downstream cellular effects in SW13/cl.2 cells, showing nitrated PLs as new potential electrophilic lipid mediators with selective actions [33]. However, their structural characteristics can have a great impact on their biological significance, as previously reported for NO 2 -FAs (reviewed in [7]).…”
Section: Discussionmentioning
confidence: 99%
“…At the time point, the cells were collected by scraping in phosphate buffered saline (PBS) on ice and centrifuged at 1000 rpm for 5 min. The cell pellets were stored at −80 • C until used [33]. All experiments were repeated three times.…”
Section: Cell Culture and Treatments Of Adrenal Carcinoma Sw13/cl2 Cmentioning
confidence: 99%
“…Therefore, it would be interesting to explore whether this reversible modification can play a protective role against more disruptive modifications such as CyPG addition. Interestingly, in vitro incubation of vimentin or a PPARγ construct with the nitrated phospholipid 1-palmitoyl-2-oleyl-phosphatidylcholine (NO2-POPC) shields their cysteine residues from alkylation [201]. Whether this is due to the occurrence of competing modifications requires further study.…”
Section: Interplay Among Post-translational Modificationsmentioning
confidence: 99%
“…Other electrophilic lipids potentially interacting with vimentin include nitrated phospholipids ( Fig. 3 ), which alter the cellular vimentin network in a manner dependent on the presence of C328, and shield this residue from alkylation in vitro [ 115 ].…”
Section: Vimentinmentioning
confidence: 99%
“…[ 91 ], and peripheral condensation, by treatment with NO 2 -POPC as in Ref. [ 115 ]. Scale bar, 20 μm.…”
Section: Introductionmentioning
confidence: 99%