Insight into polyhydroxyalkanoate (PHA) production from xylose and extracellular PHA degradation by a thermophilic Schlegelella thermodepolymerans

Zhou, Wen; Colpa, Dana I.; Permentier, Hjalmar P.; Offringa, Ruben Ate; Rohrbach, Léon; Euverink, Gert-Jan; Krooneman, Janneke

doi:10.1016/j.resconrec.2023.107006

Cited by 12 publications

(8 citation statements)

References 55 publications

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“…The utilization of xylose-rich hydrolysate to PHA has been of high interest in recent years, − but due to the difficulty of most bacteria in metabolizing xylose, there is still a lot of room to improve . Here, we show the utilization of a recently described xylose-preferring thermophilic bacteria under optimal PHA production conditions . The combination of the utilization of the xylose stream with the new ALACEN process can be of high interest to further develop better holistic valorization of biomass strategies .…”

Section: Resultsmentioning

confidence: 93%

“…The properties of the obtained PHA have been studied and compared with those of commercial xylose, which has been reported by Zhou et al (2023). 15 Both of the produced PHA from xylose-rich stream and commercial xylose are identified as poly[3-hydroxybutyrate (PHB)] by NMR (Supporting Information Figure S6). The analysis performed by thermogravimetric analysis (TGA) (Supporting Information Figure S7) and differential scanning calorimetry (DSC) (Supporting Information Figure S8) shows that the glass transition temperature (T g ) and melting temperature (T m ) of the obtained PHA are −29 and 166 °C, respectively.…”

Section: ■ Resultsmentioning

confidence: 99%

“…Xylose-utilizing bacteria are not common and even less common with industrially relevant capacities like S. thermodepolymerans, which is able to produce PHA at 50 °C with xylose as the main carbon source. , The PHA production was compared to that of using pure xylose as the sole carbon source.…”

Section: Resultsmentioning

confidence: 99%

“…14 The utilization of thermophilic bacteria with high PHA production from underutilized products like xylose offers a promising biorefinery opportunity. 15 Thermophilic bacteria are industrially relevant due to fewer problems with contamination of the culture as well as their easy blend-in in coprocesses like fermentation and enzymatic cellulose hydrolysis due to similar optimum temperatures (50 °C). 16,17 Most of the current biorefinery processes focus on recovering cellulose as fibers or as monosaccharides for fermentation.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The main reason for this is that many bacteria and yeast prefer C6 sugars over C5 sugars and usually display catabolic repression to utilize only one sugar . The utilization of thermophilic bacteria with high PHA production from underutilized products like xylose offers a promising biorefinery opportunity . Thermophilic bacteria are industrially relevant due to fewer problems with contamination of the culture as well as their easy blend-in in coprocesses like fermentation and enzymatic cellulose hydrolysis due to similar optimum temperatures (50 °C). , …”

Section: Introductionmentioning

confidence: 99%

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ALACEN: A Holistic Herbaceous Biomass Fractionation Process Attaining a Xylose-Rich Stream for Direct Microbial Conversion to Bioplastics

Bertran-Llorens,

Zhou,

Palazzolo

et al. 2024

ACS Sustainable Chem. Eng.

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Lignocellulose biorefining is a promising technology for the sustainable production of chemicals and biopolymers. Usually, when one component is focused on, the chemical nature and yield of the others are compromised. Thus, one of the bottlenecks in biomass biorefining is harnessing the maximum value from all of the lignocellulosic components. Here, we describe a mild stepwise process in a flow-through setup leading to separate flow-out streams containing cinnamic acid derivatives, glucose, xylose, and lignin as the main components from different herbaceous sources. The proposed process shows that minimal degradation of the individual components and conservation of their natural structure are possible. Under optimized conditions, the following fractions are produced from wheat straw based on their respective contents in the feed by the ALkaline ACid ENzyme process: (i) 78% ferulic acid from a mild ALkali step, (ii) 51% monomeric xylose free of fermentation inhibitors by mild ACidic treatment, (iii) 82% glucose from ENzymatic degradation of cellulose, and (iv) 55% native-like lignin. The benefits of using the flow-through setup are demonstrated. The retention of the lignin aryl ether structure was confirmed by HSQC NMR, and this allowed monomers to form from hydrogenolysis. More importantly, the crude xylose-rich fraction was shown to be suitable for producing polyhydroxybutyrate bioplastics. The direct use of the xylose-rich fraction by means of the thermophilic bacteria Schlegelella thermodepolymerans matched 91% of the PHA produced with commercial pure xylose, achieving 138.6 mgPHA/gxylose. Overall, the ALACEN fractionation method allows for a holistic valorization of the principal components of herbaceous biomasses.

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