Insight into microbial community diversity and composition of two-stage anaerobic digestion: Focusing methanogenic stage

Hailu, Abrha Mulu; Palani, Sankar Ganesh; Leta, Seyoum; Tegaye, Tenalem Ayenew

doi:10.1016/j.biteb.2021.100764

Cited by 12 publications

(5 citation statements)

References 42 publications

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“…AD is achieved through the microorganisms interaction between bacteria and archaea, so microbial structural composition is closely related to methane production. , As seen in Figure A, the relative abundance of several common microorganisms in the AD reaction, such as Spirochaetes, Planctomycetes, Chlamydiae, Lentisphaerae, and Nanoarchaeota, , showed a trend of increasing first and then decreasing with the concentration of MOF-808. Their maximum abundance appeared at 0.5 g/L.…”

Section: Resultsmentioning

confidence: 99%

High Proton Conductivity of MOF-808 Promotes Methane Production in Anaerobic Digestion

Chen

Dai

et al. 2022

ACS Sustainable Chem. Eng.

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Metal−organic frameworks (MOFs) are widely used in fuel cells, catalysis, and other fields. However, the influence and mechanism of the excellent properties of MOFs on biomethane production are still unclear. In this paper, MOF-808 was used to study anaerobic digestion (AD) of corn stalk, which increased biogas production and methane content by 11.06−39.82% and 10.15−14.28%, respectively. The optimal effect of biogas production was reached by adding 0.5 g/L MOF-808. The −OH of MOF-808 provided protons through protonation during AD, and then, a continuous hydrogen bond network was formed in the channels of frameworks. The hydrogen bond network led to the appearance of the Grotthuss mechanism, which made the dispersed H + in the system to rapidly transport to the outside of the methanogen membrane along the inner surface of the sample and the nanopores, and promoted interspecies hydrogen transfer. The formation of a transmembrane proton gradient improved the hydrogen metabolism level of methanogens and promoted the energy synthesis of microorganisms. In addition, MOF-808 had a positive impact on the community and function of the microorganisms. Due to the stable structure of MOF-808, the reflux of anaerobically digested effluent is a potential way of MOF-808 recycling.

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Section: Resultsmentioning

confidence: 99%

High Proton Conductivity of MOF-808 Promotes Methane Production in Anaerobic Digestion

Chen

Dai

et al. 2022

ACS Sustainable Chem. Eng.

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“…Many studies could have benefited from using our tool to support exploration or validation tasks in various contexts. For example, Hailu et al, 2021 focused on the methanogenic stage of microbial communities, i.e., microbes responsible for the biomethanation of the substrates. Related to the class Methanomicrobia use case and out of the methanogens genera, they found that Methanosa and Methanosarcina were the most dominant in this biochemical activity.…”

Section: Discussionmentioning

confidence: 99%

“…They are distinguished by the type of metabolite they depend on. Indeed, both genera fall within the same order known as Methanosarcinales and mainly use acetate, while the Methanobacterium belongs to the Methanobacteriales order and mainly uses dihydrogen ( Hailu et al, 2021 ). Further examples could provide important context and support domain experts in their tasks.…”

Section: Discussionmentioning

confidence: 99%

Context-Aware Phylogenetic Trees for Phylogeny-Based Taxonomy Visualization

et al. 2022

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Sustained efforts in next-generation sequencing technologies are changing the field of taxonomy. The increase in the number of resolved genomes has made the traditional taxonomy of species antiquated. With phylogeny-based methods, taxonomies are being updated and refined. Although such methods bridge the gap between phylogeny and taxonomy, phylogeny-based taxonomy currently lacks interactive visualization approaches. Motivated by enriching and increasing the consistency of evolutionary and taxonomic studies alike, we propose Context-Aware Phylogenetic Trees (CAPT) as an interactive web tool to support users in exploration- and validation-based tasks. To complement phylogenetic information with phylogeny-based taxonomy, we offer linking two interactive visualizations which compose two simultaneous views: the phylogenetic tree view and the taxonomic icicle view. Thanks to its space-filling properties, the icicle visualization follows the intuition behind taxonomies where different hierarchical rankings with equal number of child elements can be represented with same-sized rectangular areas. In other words, it provides partitions of different sizes depending on the number of elements they contain. The icicle view integrates seven taxonomic rankings: domain, phylum, class, order, family, genus, and species. CAPT enriches the clades in the phylogenetic tree view with context from the genomic data and supports interactive techniques such as linking and brushing to highlight correspondence between the two views. Four different use cases, extracted from the Genome Taxonomy DataBase, were employed to create four scenarios using our approach. CAPT was successfully used to explore the phylogenetic trees as well as the taxonomic data by providing context and using the interaction techniques. This tool is essential to increase the accuracy of categorization of newly identified species and validate updated taxonomies. The source code and data are freely available at https://github.com/ghattab/CAPT.

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“…At the phylum level, firmicutes, bacteroidetes, chloroflexi, synergistetes, patescibacteria, and cloacimonetes stood out in the community structure with the former three phyla dominating. These bacterial phyla were also found in various substrates of AD, for instance, in one-stage AD of pig slurry with fruit-vegetable waste (Ros et al, 2017) and other biogas reactors (Stets et al, 2014;Jabari et al, 2016) as well as in two-stage AD of abattoir wastewater, heterogeneous fruit-vegetable solid waste and their combinations (Hailu et al, 2021), though the phyla proteobacteria and firmicutes dominated in AD of the substrates not rich in proteins.…”

Section: (B) Bacterial Community Structure Analysismentioning

confidence: 94%

Effect of Thermal Hydrolysis Pretreatment on Anaerobic Digestion of Protein-Rich Biowaste: Process Performance and Microbial Community Structures Shift

Shi

Zhang

et al. 2022

Front. Environ. Sci.

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To reveal the effects of thermal hydrolysis (TH) pretreatment (THPT) on anaerobic digestion (AD) of protein-rich substrates, discarded tofu was chosen as the object, and its batch AD tests of tofu before and after being subjected to TH at gradually increasing organic loads were carried out and the AD process characteristics were compared; furthermore, its continuous AD tests without and with THPT were also conducted and the difference of the microbial community structures was investigated. The results showed that, during AD of protein-rich tofu with increase in the organic load, inhibition from severe acidification prior to accumulation of ammonia nitrogen (AN) occurred. THPT helped overcome the acidification inhibition present in batch AD of tofu at such a high TS content of 3.6%, to obtain the maximum methane yield rate of 589.39 ml·(gVS)−1. Continuous AD of protein-rich tofu heavily depended on ammonia-tolerant hydrogenotrophic methanogens and bacteria. The continuous AD processes acclimated by HT substrates seemed to be resistant to severe organic loads, by boosting growth of ammonia-tolerant microorganisms, above all methylotrophic methanogens such as the genera RumEnM2 and methanomassiliicoccus. The process response of continuous AD of HT tofu was hysteretic, suggesting that a sufficiently long adaptation period was required for stabilizing the AD system.

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Insight into microbial community diversity and composition of two-stage anaerobic digestion: Focusing methanogenic stage

Cited by 12 publications

References 42 publications

High Proton Conductivity of MOF-808 Promotes Methane Production in Anaerobic Digestion

High Proton Conductivity of MOF-808 Promotes Methane Production in Anaerobic Digestion

Context-Aware Phylogenetic Trees for Phylogeny-Based Taxonomy Visualization

Effect of Thermal Hydrolysis Pretreatment on Anaerobic Digestion of Protein-Rich Biowaste: Process Performance and Microbial Community Structures Shift

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