Insight into levofloxacin loaded biocompatible electrospun scaffolds for their potential as conjunctival substitutes

Biomaterials and tissue engineering strategies for posterior lamellar eyelid reconstruction: Replacement or regeneration?

“…It has been considered a novel direction of tissue engineering to blend PLA with other biomaterials with better wettability, which could result in better tissue recovery. Yan et al 12 developed a PLA electrospun nanofibrous membrane scaffold for conjunctival repair, which was coated with cellulose nanofibrils to improve hydrophilicity, coated with silk peptide to promote conjunctival epithelial cell proliferation, and loaded with levofloxacin to prevent postoperative infection. By promoting the restoration of conjunctival structure and function, this scaffold yielded favorable therapeutic effects after transplantation in a rabbit conjunctival defect model 12 .…”

“…Yan et al 12 developed a PLA electrospun nanofibrous membrane scaffold for conjunctival repair, which was coated with cellulose nanofibrils to improve hydrophilicity, coated with silk peptide to promote conjunctival epithelial cell proliferation, and loaded with levofloxacin to prevent postoperative infection. By promoting the restoration of conjunctival structure and function, this scaffold yielded favorable therapeutic effects after transplantation in a rabbit conjunctival defect model 12 . In addition, to improve the hydrophobicity and modulate the biomechanical property of pure PPF polymer, a degradable and mechanically adjustable scaffold was synthesized by copolymerizing PPF with 2‐hydroxyethyl methacrylate (HEMA), a widely used hydrophilic monomer.…”

Advances in Nanostructures for Antimicrobial Therapy

“…Bacterial cellulose (BC)–CS NPs composite hydrogels fabricated by γ-irradiation (20–60 kGy) showed superb antibiofilm properties, reflected in up to 90% reduction of viable biofilm and up to 65% reduction of biofilm height and was characterized by a higher porosity, a higher wound fluid absorption and faster in vitro healing compared to respective composite hydrogels prepared with CS polymer [ 239 ]. LEV-loaded composite scaffolds of PLA electrospun nanofibrous membranes surface-coated by CNFs and/or silk peptide, in which the CNF coating ameliorated the hydrophilicity and silk peptide coating proliferated conjunctival epithelial cells exhibiting effective bactericidal effects; they were able to stimulate structural and functional restoration of conjunctiva after transplant and thus minimize the post-surgery application of antibiotics [ 240 ]. Ampicillin-encapsulated BC/PVA hydrogels ensured 30% of the cumulative antibiotic delivery during 120 h, exhibited superior antibacterial activity against S. aureus and E. coli and can be used as wound dressings with sustained antibiotic delivery [ 241 ].…”

Silk Fibroin Combined with Electrospinning as a Promising Strategy for Tissue Regeneration

“…[ 18,19 ] Electrospun silk fibroin nanofibers not only retain the excellent biological properties of silk fibroin, but also have a 3D porous structure that can highly imitate the structure of the extracellular matrix and promote tissue regeneration. [ 20,21 ]…”

“…[18,19] Electrospun silk fibroin nanofibers not only retain the excellent biological properties of silk fibroin, but also have a 3D porous structure that can highly imitate the structure of the extracellular matrix and promote tissue regeneration. [20,21] In recent years, many studies on the tissue regeneration of electrospun SF nanofibers have been impressive, possibly representing an important breakthrough direction in tissue engineering. [22][23][24] Nevertheless, it is regrettable that no relevant review has yet organized and discussed these studies.…”