Insight into Differential Responses of Upland and Paddy Rice to Drought Stress by Comparative Expression Profiling Analysis

Ding, Xuezhi; Li, Xiaokai; Xiong, Lizhong

doi:10.3390/ijms14035214

Cited by 46 publications

(27 citation statements)

References 41 publications

(52 reference statements)

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“…Previous studies reported comparative expression profiles of UR and LR under drought stress conditions using cDNA microarray technology (Ding et al ., ; Lenka et al ., ; Wang et al ., ). In addition, differently expressed genes in the two genotypes were identified, and several of them were currently proved to be involved in drought response.…”

Section: Discussionmentioning

confidence: 99%

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OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H₂O₂ signalling in rice

Yang

Zhen

et al. 2016

Plant Biotechnology Journal

193

134

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SummaryDrought is one of the major abiotic stresses that directly implicate plant growth and crop productivity. Although many genes in response to drought stress have been identified, genetic improvement to drought resistance especially in food crops is showing relatively slow progress worldwide. Here, we reported the isolation of abscisic acid, stress and ripening (ASR) genes from upland rice variety, IRAT109 (Oryza sativa L. ssp. japonica), and demonstrated that overexpression of OsASR5 enhanced osmotic tolerance in Escherichia coli and drought tolerance in Arabidopsis and rice by regulating leaf water status under drought stress conditions. Moreover, overexpression of OsASR5 in rice increased endogenous ABA level and showed hypersensitive to exogenous ABA treatment at both germination and postgermination stages. The production of H2O2, a second messenger for the induction of stomatal closure in response to ABA, was activated in overexpression plants under drought stress conditions, consequently, increased stomatal closure and decreased stomatal conductance. In contrast, the loss‐of‐function mutant, osasr5, showed sensitivity to drought stress with lower relative water content under drought stress conditions. Further studies demonstrated that OsASR5 functioned as chaperone‐like protein and interacted with stress‐related HSP40 and 2OG‐Fe (II) oxygenase domain containing proteins in yeast and plants. Taken together, we suggest that OsASR5 plays multiple roles in response to drought stress by regulating ABA biosynthesis, promoting stomatal closure, as well as acting as chaperone‐like protein that possibly prevents drought stress‐related proteins from inactivation.

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Section: Discussionmentioning

confidence: 99%

“…Previous studies reported comparative expression profiles of UR and LR under drought stress conditions using cDNA microarray technology(Ding et al, 2013;Lenka et al, 2011; …”

mentioning

confidence: 99%

OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H₂O₂ signalling in rice

Yang

Zhen

et al. 2016

Plant Biotechnology Journal

193

134

View full text Add to dashboard Cite

show abstract

“…Defense regulators were annotated based on literature (Vergne et al, 2008). Gene expressed in drought stress were annotated based on literature on rice microarray under this stress (Rabbani et al, 2003; Zhang et al, 2012; Ding et al, 2013; Narsai et al, 2013). …”

Section: Methodsmentioning

confidence: 99%

Transcriptional Basis of Drought-Induced Susceptibility to the Rice Blast Fungus Magnaporthe oryzae

et al. 2016

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Plants are often facing several stresses simultaneously. Understanding how they react and the way pathogens adapt to such combinational stresses is poorly documented. Here, we developed an experimental system mimicking field intermittent drought on rice followed by inoculation by the pathogenic fungus Magnaporthe oryzae. This experimental system triggers an enhancement of susceptibility that could be correlated with the dampening of several aspects of plant immunity, namely the oxidative burst and the transcription of several pathogenesis-related genes. Quite strikingly, the analysis of fungal transcription by RNASeq analysis under drought reveals that the fungus is greatly modifying its virulence program: genes coding for small secreted proteins were massively repressed in droughted plants compared to unstressed ones whereas genes coding for enzymes involved in degradation of cell-wall were induced. We also show that drought can lead to the partial breakdown of several major resistance genes by affecting R plant gene and/or pathogen effector expression. We propose a model where a yet unknown plant signal can trigger a change in the virulence program of the pathogen to adapt to a plant host that was affected by drought prior to infection.

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“…Applying hierarchical clustering to drought-treated upland and paddy rice identified 213 candidate genes that colocalized with 17 QTL intervals [69]. These clustering methods have been widely used in expression analysis to generate lists of similarly responding genes.…”

Section: Current Opinion In Plant Biologymentioning

confidence: 99%

Transcriptional networks — crops, clocks, and abiotic stress

Gehan

Greenham

Mockler

et al. 2015

Current Opinion in Plant Biology

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Several factors affect the yield potential and geographical range of crops including the circadian clock, water availability, and seasonal temperature changes. In order to sustain and increase plant productivity on marginal land in the face of both biotic and abiotic stresses, we need to more efficiently generate stress-resistant crops through marker-assisted breeding, genetic modification, and new genome-editing technologies. To leverage these strategies for producing the next generation of crops, future transcriptomic data acquisition should be pursued with an appropriate temporal design and analyzed with a network-centric approach. The following review focuses on recent developments in abiotic stress transcriptional networks in economically important crops and will highlight the utility of correlation-based network analysis and applications.

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Insight into Differential Responses of Upland and Paddy Rice to Drought Stress by Comparative Expression Profiling Analysis

Cited by 46 publications

References 41 publications

OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H₂O₂ signalling in rice

OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H₂O₂ signalling in rice

Transcriptional Basis of Drought-Induced Susceptibility to the Rice Blast Fungus Magnaporthe oryzae

Transcriptional networks — crops, clocks, and abiotic stress

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Insight into Differential Responses of Upland and Paddy Rice to Drought Stress by Comparative Expression Profiling Analysis

Cited by 46 publications

References 41 publications

OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signalling in rice

OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signalling in rice

Transcriptional Basis of Drought-Induced Susceptibility to the Rice Blast Fungus Magnaporthe oryzae

Transcriptional networks — crops, clocks, and abiotic stress

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Product

Resources

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OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H₂O₂ signalling in rice

OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H₂O₂ signalling in rice