Inside Back Cover: A DNA Barcode‐Based Aptasensor Enables Rapid Testing of Porcine Epidemic Diarrhea Viruses in Swine Saliva Using Electrochemical Readout (Angew. Chem. Int. Ed. 31/2022)

Abstract

Rapid and reagent-free pathogen tests … …a re urgently needed. In their Research Article (e202204252), Leyla Soleymani, Yingfu Li and co-workers developed adual-electrode electrochemical chip (DEE-Chip) and ab arcode-releasing electroactive aptamer for rapid on-farm detection of porcine epidemic diarrhea viruses (PEDv). In contrast to current biosecurity surveillance with turnaround times of 2-4 days,this technology identifies infection with PEDv in pig saliva in one hour with adiagnostic sensitivity of 100 %and specificity of 83 %.

Development of Nucleic‐Acid‐Based Electrochemical Biosensors for Clinical Applications

“…In this assay, binding‐induced strand displacement was used to release a redox DNA reporter on one electrode. This reporter strand is then re‐captured on another closely spaced electrode for signal transduction (Figure 4D) [73] . This study presents a simple and reagent‐free assay that detects PEDv in an hour with an LOD of 6 nM using PEDv N‐protein spiked in swine saliva.…”

“…[104] Future work should focus on reducing the sample-to-result-time of these DNA-based assays, which can be achieved using electric-field-mediated mass transport of the DNA reporter from the release to the capture electrode. [73]…”

“…frequency). [73,89,232,233] The molecular pendulum assay discussed in Figure 4C was also used for detecting troponin. [71] This study was first validated using spiked troponin in saliva, tear, urine, sweat and blood.…”

“…Synthetic DNA molecules have also been exploited as reporting probes (Figure 2B) because they can be easily modified with both biorecognition elements (such as antibodies) and reporter labels (such as redox molecules, fluorophores, or Raman probes) [68] . Additionally, inherent properties of nucleic acids, such as steric hindrance [69] and electrostatic repulsion on a surface (Figure 2B i), [70] molecular dynamics on a surface (Figure 2B ii), [71] and target‐induced strand displacement (Figure 2B iii), [72, 73] are strategically used to build molecular systems that translate target binding to a detectible signal. Such systems have been used in recent years to streamline antibody‐based assays for use in clinical and wearable settings [34, 71, 74–76] …”

Development of Nucleic‐Acid‐Based Electrochemical Biosensors for Clinical Applications

“…Synthetic DNA molecules have also been exploited as reporting probes (Figure 2B) because they can be easily modified with both biorecognition elements (such as antibodies) and reporter labels (such as redox molecules, fluorophores, or Raman probes) [68] . Additionally, inherent properties of nucleic acids, such as steric hindrance [69] and electrostatic repulsion on a surface (Figure 2B i), [70] molecular dynamics on a surface (Figure 2B ii), [71] and target‐induced strand displacement (Figure 2B iii), [72, 73] are strategically used to build molecular systems that translate target binding to a detectible signal. Such systems have been used in recent years to streamline antibody‐based assays for use in clinical and wearable settings [34, 71, 74–76] …”