Insertional mutagenesis and characterization of a polyketide synthase gene (<i>PKS1</i>) required for melanin biosynthesis in<i>Bipolaris oryzae</i>

Moriwaki, Akihiro; Kihara, Junichi; Kobayashi, Tsutomu; Tokunaga, Toshiko; Arase, Seiji; Honda, Yuichi

doi:10.1111/j.1574-6968.2004.tb09729.x

Cited by 33 publications

(59 citation statements)

References 31 publications

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“…SNOG_11981.2 is supported by five ESTs from the in planta library and none from the oleate library, consistent with upregulation during infection and sporulation (no other PKSs have EST support). This gene is orthologous to nonreducing clade 2 PKS genes that are associated with 1,8-dihydroxynaphthalene melanin biosynthesis in Bipolaris oryzae and many other pathogens (Kroken et al, 2003;Moriwaki et al, 2004;Amnuaykanjanasin et al, 2005). We showed previously that S. nodorum produces melanin from dihydroxyphenlyalanine (Solomon et al, 2004b), for which a PKS would not be necessary.…”

Section: Functional Analysis Of Proteinsmentioning

confidence: 99%

Dothideomycete–Plant Interactions Illuminated by Genome Sequencing and EST Analysis of the Wheat Pathogen Stagonospora nodorum

et al. 2007

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Stagonospora nodorum is a major necrotrophic fungal pathogen of wheat (Triticum aestivum) and a member of the Dothideomycetes, a large fungal taxon that includes many important plant pathogens affecting all major crop plant families. Here, we report the acquisition and initial analysis of a draft genome sequence for this fungus. The assembly comprises 37,164,227 bp of nuclear DNA contained in 107 scaffolds. The circular mitochondrial genome comprises 49,761 bp encoding 46 genes, including four that are intron encoded. The nuclear genome assembly contains 26 classes of repetitive DNA, comprising 4.5% of the genome. Some of the repeats show evidence of repeat-induced point mutations consistent with a frequent sexual cycle. ESTs and gene prediction models support a minimum of 10,762 nuclear genes. Extensive orthology was found between the polyketide synthase family in S. nodorum and Cochliobolus heterostrophus, suggesting an ancient origin and conserved functions for these genes. A striking feature of the gene catalog was the large number of genes predicted to encode secreted proteins; the majority has no meaningful similarity to any other known genes. It is likely that genes for host-specific toxins, in addition to ToxA, will be found among this group. ESTs obtained from axenic mycelium grown on oleate (chosen to mimic early infection) and late-stage lesions sporulating on wheat leaves were obtained. Statistical analysis shows that transcripts encoding proteins involved in protein synthesis and in the production of extracellular proteases, cellulases, and xylanases predominate in the infection library. This suggests that the fungus is dependant on the degradation of wheat macromolecular constituents to provide the carbon skeletons and energy for the synthesis of proteins and other components destined for the developing pycnidiospores.

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Section: Functional Analysis Of Proteinsmentioning

confidence: 99%

Dothideomycete–Plant Interactions Illuminated by Genome Sequencing and EST Analysis of the Wheat Pathogen Stagonospora nodorum

et al. 2007

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“…According to the rational that mutants could be distinguished by mycelial color change, we chose a polyketide synthase gene (pks-1) as a target to make a knock-down mutant, as it shares similarity to PKSs involved in melanin formation. The molecular genetics of polyketide synthases involved in melanin biosynthesis has been studied in many fungi, including Sordaria macrospora [33], Bipolaris oryzae [34] and Colletotrichum lagenarium [35]. The C. globosum pks-1 gene encodes a PKS that shares highest similarity to the melanin PKS from Neurospora tetrasperma (EGO61342.1, with 65% identity).…”

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confidence: 99%

A PKS gene, pks-1, is involved in chaetoglobosin biosynthesis, pigmentation and sporulation in Chaetomium globosum

Hao

Lou

et al. 2012

Sci. China Life Sci.

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Chaetomium globosum is one of the most common fungi in nature. It is best known for producing chaetoglobosins; however, the molecular basis of chaetoglobosin biosynthesis is poorly understood in this fungus. In this study, we utilized RNA interference (RNAi) to characterize a polyketide synthase gene, pks-1, in C. globosum that is involved in the production of chaetoglobosin A. When pks-1 was knocked down by RNAi, the production of chaetoglobosin A dramatically decreased. Knock-down mutants also displayed a pigment-deficient phenotype. These results suggest that the two polyketides, melanin and chaetoglobosin, are likely to share common biosynthetic steps. Most importantly, we found that pks-1 also plays a critical role in sporulation. The silenced mutants of pks-1 lost the ability to produce spores. We propose that polyketides may modulate cellular development via an unidentified action. We also suggest that C. globosum pks-1 is unique because of its triple role in melanin formation, chaetoglobosin biosynthesis and sporulation. This work may shed light on chaetoglobosin biosynthesis and indicates a relationship between secondary metabolism and fungal morphogenesis.

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“…Direct DNA transfer has been, and is still being, used successfully as a method for random mutagenesis in fungi [50][51][52][53]. As DNA that is not homologous to the recipient genome appears to integrate approximately at random [54], thousands of individual transformants collectively provide a library of tagged mutations.…”

Section: Gene Tagging By Direct Dna Transfermentioning

confidence: 99%

Approaches to functional genomics in filamentous fungi

et al. 2006

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The study of gene function in filamentous fungi is a field of research that has made great advances in very recent years. A number of transformation and gene manipulation strategies have been developed and applied to a diverse and rapidly expanding list of economically important filamentous fungi and oomycetes. With the significant number of fungal genomes now sequenced or being sequenced, functional genomics promises to uncover a great deal of new information in coming years. This review discusses recent advances that have been made in examining gene function in filamentous fungi and describes the advantages and limitations of the different approaches.

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Insertional mutagenesis and characterization of a polyketide synthase gene (PKS1) required for melanin biosynthesis inBipolaris oryzae

Cited by 33 publications

References 31 publications

Dothideomycete–Plant Interactions Illuminated by Genome Sequencing and EST Analysis of the Wheat Pathogen Stagonospora nodorum

Dothideomycete–Plant Interactions Illuminated by Genome Sequencing and EST Analysis of the Wheat Pathogen Stagonospora nodorum

A PKS gene, pks-1, is involved in chaetoglobosin biosynthesis, pigmentation and sporulation in Chaetomium globosum

Approaches to functional genomics in filamentous fungi

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