Insertion of T4-lysozyme (T4L) can be a useful tool for studying olfactory-related GPCRs

Corin, Karolina; Pick, Horst; Philipp, B.; Cook, Brian L.; Duhr, Stefan; Wienken, Christoph J.; Braun, Dieter; Vogel, Horst; Zhang, Shuguang

doi:10.1039/c2mb05495g

Cited by 10 publications

(6 citation statements)

References 35 publications

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“…In other work, we used CD for studying integral membrane proteins, particularly G protein‐coupled receptors (GPCRs), including olfactory receptors, formyl peptide receptors, and trace amine associated receptors (Figures ) . These proteins are predominately composed of seven transmembrane α‐helical segments.…”

Section: Other Aspects Of Studying Membrane Proteins Using CDmentioning

confidence: 99%

Discovery of the first self‐assembling peptide, study of peptide dynamic behaviors, and G protein‐coupled receptors using an Aviv circular dichroism spectropolarimeter

Zhang

2018

Biopolymers

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Circular dichroism (CD) spectroscopy is a useful technique to study the structure and dynamics of peptides, proteins and nucleic acids. CD is particularly useful because sample volumes may be as low as 50 μL, it provides high precision and sensitivity, and it achieves a good signal to noise ratio. CD characterizes molecular conformational changes in real time by finely controlling temperature, pH, and titrating urea and guanidine·HCl which is necessary for studying protein folding. Although CD does not provide detailed structure at the atomic level, it provides a global structural framework. Researchers use CD to observe molecular phenomena, namely how macromolecules unfold/refold and their overall self‐assembly/disassembly. Using CD to monitor a peptide structure, I serendipitously discovered the self‐assembling peptide EAK16 from yeast protein Zuotin. This unusual peptide formed a new type of nanofiber scaffold hydrogel material. The discovery in 1990 opened a new field in the design and study of numerous self‐assembling peptides, thereby launching the area of peptide nanobiotechnology. In this review, I reflect on my personal discoveries of several self‐assembling peptides, investigations into the dynamic behaviors of peptides, as well as the impact of the work on society. I also describe studies of natural membrane proteins and engineered membrane proteins using CD. Furthermore, I enjoyed numerous and close interactions with Jack Aviv since 1997. He generously supported 10 high impact workshops (Crete and Mikonos) and meetings in various countries around the world that left fond memories of many young researches who later became leading scientists in their respective fields.

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Section: Other Aspects Of Studying Membrane Proteins Using CDmentioning

confidence: 99%

Discovery of the first self‐assembling peptide, study of peptide dynamic behaviors, and G protein‐coupled receptors using an Aviv circular dichroism spectropolarimeter

Zhang

2018

Biopolymers

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“…Three major distinct types of lysozyme have been identified: chicken-type (c-type), invertebrate-type (i-type), and goose-type (g-type) lysozyme ( Callewaert and Michiels, 2010 ). As a natural non-specific defense factor in humans and animals, lysozyme can destroy the cell walls of Gram positive bacteria by hydrolyzing the link of β-1,4 glycosidic bonds between the peptidoglycan N-acetyl glucosamine and the N-acetylmuramic acid, thus damaging the bracket of the peptidoglycan causing the cells to disintegrate by internal osmotic pressure ( Irwin, 2004 ; Corin et al, 2012 ; Kastorna et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Characterization of a New C-type Lysozyme Gene from Yak Mammary Tissue

Jiang

Ren

et al. 2015

Asian Australas. J. Anim. Sci

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Milk lysozyme is the ubiquitous enzyme in milk of mammals. In this study, the cDNA sequence of a new chicken-type (c-type) milk lysozyme gene (YML), was cloned from yak mammary gland tissue. A 444 bp open reading frames, which encodes 148 amino acids (16.54 kDa) with a signal peptide of 18 amino acids, was sequenced. Further analysis indicated that the nucleic acid and amino acid sequences identities between yak and cow milk lysozyme were 89.04% and 80.41%, respectively. Recombinant yak milk lysozyme (rYML) was produced by Escherichia coli BL21 and Pichia pastoris X33. The highest lysozyme activity was detected for heterologous protein rYML5 (M = 1,864.24 U/mg, SD = 25.75) which was expressed in P. pastoris with expression vector pPICZαA and it clearly inhibited growth of Staphylococcus aureus. Result of the YML gene expression using quantitative polymerase chain reaction showed that the YML gene was up-regulated to maximum at 30 day postpartum, that is, comparatively high YML can be found in initial milk production. The phylogenetic tree indicated that the amino acid sequence was similar to cow kidney lysozyme, which implied that the YML may have diverged from a different ancestor gene such as cow mammary glands. In our study, we suggest that YML be a new c-type lysozyme expressed in yak mammary glands that plays a role as host immunity.

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“…Later we expanded to other GPCRs including other sensory receptors. [74][75][76][77][78][79][80][81][82] F I G U R E 1 5 Amino acids in proteins. (a) 1.5 Å resolution electron density maps of the 20 amino acids from X-ray diffraction.…”

Section: Beyond Self-assembling Peptidesmentioning

confidence: 99%

Self‐assembling peptides: From a discovery in a yeast protein to diverse uses and beyond

Zhang

2020

Protein Science

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Well-defined nanofiber scaffold hydrogels made of self-assembling peptides have found their way into various 3D tissue culture and clinical products. I reflect initial puzzlement of the unexpected discovery, gradual understanding of how these peptides undergo self-assembly, to eventually translating designer biological scaffolds into commercial products. Peptides are ubiquitous in nature and useful in many fields. They are found as hormones, pheromones, antibacterial, and antifungal agents in innate immunity systems, toxins, as well anti-inset pesticides. However, the concept of peptides as materials was not recognized until 1990 when a self-assembling peptide as a repeating segment in a yeast protein was serendipitously discovered. The peptide materials have bona fide materials properties and are made from simple amino acids with well-ordered nanostructures under physiological conditions. Some current applications include: (a) Real 3D tissue cell cultures of diverse tissue cells and various stem cells; (b) reparative and regenerative medicine as well as tissue engineering; (c) 3D tissue printing; (d) sustained releases of small molecules, growth factors and monoclonal antibodies; and (e) accelerated wound healing of skin and diabetic ulcers as well as instant hemostasis in surgery. Self-assembling peptide nanobiotechnology will likely continue to expand in many directions in the coming years. I will also briefly introduce my current research using a simple QTY code for membrane protein design. I am greatly honored and humbled to be invited to contribute an Award Winner Recollection of the 2020 Emil Thomas Kaiser Award from the Protein Society.

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Insertion of T4-lysozyme (T4L) can be a useful tool for studying olfactory-related GPCRs

Cited by 10 publications

References 35 publications

Discovery of the first self‐assembling peptide, study of peptide dynamic behaviors, and G protein‐coupled receptors using an Aviv circular dichroism spectropolarimeter

Discovery of the first self‐assembling peptide, study of peptide dynamic behaviors, and G protein‐coupled receptors using an Aviv circular dichroism spectropolarimeter

Molecular Cloning and Characterization of a New C-type Lysozyme Gene from Yak Mammary Tissue

Self‐assembling peptides: From a discovery in a yeast protein to diverse uses and beyond

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