Insertion mode of a novel anionic antimicrobial peptide MDpep5 (Val-Glu-Ser-Trp-Val) from Chinese traditional edible larvae of housefly and its effect on surface potential of bacterial membrane

Tang, Yali; Shi, Yonghui; Zhao, Wei; Hao, Gang; Li, Guowei

doi:10.1016/j.jpba.2008.09.006

Cited by 47 publications

(33 citation statements)

References 46 publications

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“…Based on the literature, when we review the results of present study, it appears that at physiological pH the carboxylate ions on the G 3.5 -PAMAM-COOH act as polyanions interacting with the calcium ions in the outer membrane of gram negative bacteria affecting its permeability. As compared to the anionic G 3.5 -PAMAM-COOH, the cationic G 4 -PAMAM-NH 2 causes more damage to outer membrane structure, which is again consistent with the observations reported by Tang et al (Tang et al, 2008). From our studies we found that PAMAM dendrimers with amine, hydroxyl and carboxyl terminal group, could each show a different path for interacting with the bacterial outer membrane.…”

Section: Resultssupporting

confidence: 91%

“…Further, anionic peptide MDpep5 acts as antimicrobial by binding to the bacteria through hydrophobic interactions causing disorders in bilayer structure. The membrane disruption is not complete for anionic MDpep5 when compared to the cationic (AMPs) antimicrobial peptide (Tang et al, 2008). Based on the literature, when we review the results of present study, it appears that at physiological pH the carboxylate ions on the G 3.5 -PAMAM-COOH act as polyanions interacting with the calcium ions in the outer membrane of gram negative bacteria affecting its permeability.…”

Section: Resultsmentioning

confidence: 99%

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Inhibition of bacterial growth and intramniotic infection in a guinea pig model of chorioamnionitis using PAMAM dendrimers

Wang

Navath

Menjoge

et al. 2010

International Journal of Pharmaceutics

134

100

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Dendrimers have emerged as topical microbicides to treat vaginal infections. This study explores the in-vitro, in-vivo antimicrobial activity of PAMAM dendrimers, and the associated mechanism. Interestingly, topical cervical application of 500 µg of generation-4 neutral dendrimer (G4-PAMAM-OH) showed potential to treat the Escherichia coli induced ascending uterine infection in guinea pig model of chorioamnionitis. Amniotic fluid collected from different gestational sacs of infected guinea pigs post treatment showed absence of E. coli growth in the cultures plated with it. The cytokine level [tumor necrosis factor (TNFα) and interleukin (IL-6 and IL-1β)] in placenta of the G4-PAMAM-OH treated animals were comparable to those in healthy animals while these were notably high in infected animals. Since, antibacterial activity of amine-terminated PAMAM dendrimers is known, the activity of hydroxyl and carboxylic acid terminated PAMAM dendrimers was compared with it. Though the G4-PAMAM-NH2 shows superior antibacterial activity, it was found to be cytotoxic to human cervical epithelial cell line above 10µg / mL, while the G4-PAMAM-OH was non cytotoxic upto 1mg / mL concentration. Cell integrity, outer (OM) and inner (IM) membrane permeabilization assays showed that G4-PAMAM-OH dendrimer efficiently changed the OM permeability, while G4-PAMAM-NH2 and G3.5-PAMAM-COOH damaged both OM and IM causing the bacterial lysis. The possible antibacterial mechanism are; G4-PAMAM-NH2 acts as polycation binding to the polyanionic lipopolysaccharide in E. coli, the G4-PAMAM-OH forms hydrogen bonds with the hydrophilic O-antigens in E. coli membrane and the G3.5-PAMAM-COOH acts as a polyanion, chelating the divalent ions in outer cell membrane of E. coli. This is the first study which shows that G4-PAMAM-OH dendrimer acts as an antibacterial agent.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Inhibition of bacterial growth and intramniotic infection in a guinea pig model of chorioamnionitis using PAMAM dendrimers

Wang

Navath

Menjoge

et al. 2010

International Journal of Pharmaceutics

134

100

View full text Add to dashboard Cite

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“…The experiment was carried out according to the method of Tang and others (2008). Logarithmic phase Shigella dysenteriae and S. pneumoniae were washed and resuspended in 10 mM PBS (pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

Antibacterial Activity and Mechanism of Action of Chlorogenic Acid

Lou

Wang

Zhu

et al. 2011

Journal of Food Science

522

310

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In this study, the antibacterial activity and mechanism of action of chlorogenic acid against bacteria were assessed. The data from minimum inhibitory concentration (MIC) values showed that chlorogenic acid effectively inhibited the growth of all tested bacterial pathogens, and the MIC values were ranging from 20 to 80 μg/mL. An investigation into action mode of chlorogenic acid against the pathogen indicated that chlorogenic acid significantly increased the outer and plasma membrane permeability, resulting in the loss of the barrier function, even inducing slight leakage of nucleotide. The leakage of cytoplasmic contents was also observed by electron micrographs. These results supported our hypothesis that chlorogenic acid bound to the outer membrane, disrupted the membrane, exhausted the intracellular potential, and released cytoplasm macromolecules, which led to cell death.

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“…After 1, 2, 4 or 6 hours of incubation, the microtubes were centrifuged (5 min at 3,000 rpm), and the absorbance of the supernatants (100 μL) was measured at 260 nm (Gene Quant DNA/RNA Eppendorf). In this assay, the absorbance due to leakage from cells treated with HClO 4 (1.2 M, 100°C, 30 min) was considered 100% [10,13]. …”

Section: Methodsmentioning

confidence: 99%

Antifungal activity of synthetic naphthoquinones against dermatophytes and opportunistic fungi: preliminary mechanism-of-action tests

Ferreira

Cardoso

Silva

et al. 2014

Ann Clin Microbiol Antimicrob

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This study evaluated the antifungal activities of synthetic naphthoquinones against opportunistic and dermatophytic fungi and their preliminary mechanisms of action. The minimum inhibitory concentrations (MICs) of four synthetic naphthoquinones for 89 microorganisms, including opportunistic yeast agents, dermatophytes and opportunistic filamentous fungi, were determined. The compound that exhibited the best activity was assessed for its action against the cell wall (sorbitol test), for interference associated with ergosterol interaction, for osmotic balance (K+ efflux) and for membrane leakage of substances that absorb at the wavelength of 260 nm. All tested naphthoquinones exhibited antifungal activity, and compound IVS320 (3a,10b-dihydro-1H-cyclopenta [b] naphtho [2,3-d] furan-5,10-dione)-dione) demonstrated the lowest MICs across the tested species. The MIC of IVS320 was particularly low for dermatophytes (values ranging from 5–28 μg/mL) and Cryptococcus spp. (3–5 μg/mL). In preliminary mechanism-of-action tests, IVS320 did not alter the fungal cell wall but did cause problems in terms of cell membrane permeability (efflux of K+ and leakage of substances that absorb at 260 nm). This last effect was unrelated to ergosterol interactions with the membrane.

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Insertion mode of a novel anionic antimicrobial peptide MDpep5 (Val-Glu-Ser-Trp-Val) from Chinese traditional edible larvae of housefly and its effect on surface potential of bacterial membrane

Cited by 47 publications

References 46 publications

Inhibition of bacterial growth and intramniotic infection in a guinea pig model of chorioamnionitis using PAMAM dendrimers

Inhibition of bacterial growth and intramniotic infection in a guinea pig model of chorioamnionitis using PAMAM dendrimers

Antibacterial Activity and Mechanism of Action of Chlorogenic Acid

Antifungal activity of synthetic naphthoquinones against dermatophytes and opportunistic fungi: preliminary mechanism-of-action tests

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