Insemination of susceptible and preimmunized gilts with boar semen containing porcine reproductive and respiratory syndrome virus

Prieto, C.; Suárez, Paula; Simarro, I.; García, C.; Martín-Rillo, S.; Castro, José María Albertos

doi:10.1016/s0093-691x(97)00023-x

Cited by 43 publications

(44 citation statements)

References 17 publications

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“…These results agree with previous findings, in which the PRRSV vaccine was more effective against homologous challenges than heterologous challenges (15,20). In addition, less protection against heterologous challenge than homologous challenge was observed in a trial that included the challenge of pregnant sows with the same vaccine used in this study (12).…”

Section: Discussionsupporting

confidence: 92%

Effects of North American Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)-Based Modified Live Vaccines on Preimmunized Sows Artificially Inseminated with European PRRSV-Spiked Semen

Han

Seo

et al. 2012

Clin. Vaccine Immunol.

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The objective of the present study was to determine if the European porcine reproductive and respiratory syndrome virus (PRRSV) can be transmitted via spiked semen to preimmunized sows and induce reproductive failure. Sows were immunized with the North American PRRSV-based modified live vaccine (Ingelvac PRRS MLV; Boehringer Ingelheim Animal Health, St. Joseph, MO) and were artificially inseminated. The sows were randomly divided into three groups. The vaccinated (group 2) and nonvaccinated (group 3) sows developed a PRRSV viremia at 7 to 28 days postinsemination with the European PRRSV-spiked semen. The number of genomic copies of the European PRRSV in serum samples was not significantly different between vaccinated and nonvaccinated sows. All negative-control sows in group 1 farrowed at the expected date. The sows in groups 2 and 3 farrowed between 103 and 110 days after the first insemination. European PRRSV RNA was detected in the lungs of 8 out of 11 live-born piglets and 46 out of 54 stillborn fetuses. In addition, PRRSV RNA was detected using in situ hybridization in other tissues from vaccinated sows that had been inseminated with European PRRSV-spiked semen (group 2). The present study has demonstrated that vaccinating sows with the North American PRRSV-based modified live vaccine does not prevent reproductive failure after insemination with European PRRSV-spiked semen.

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Section: Discussionsupporting

confidence: 92%

Effects of North American Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)-Based Modified Live Vaccines on Preimmunized Sows Artificially Inseminated with European PRRSV-Spiked Semen

Han

Seo

et al. 2012

Clin. Vaccine Immunol.

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“…Transmission of the pathogen by semen to the sow has been clearly proven for CSF [14] and PRRS virus [44] after experimental inoculation of boars, and for porcine parvovirus [30] and PRRS virus [45,46] after experimental inoculation of semen. Therefore, although contaminated semen does indeed constitute a serious risk for transmission, it does not guarantee that transmission of these pathogens to the sow by AI will consistently occur (e.g.…”

Section: Effects Of Microbial Contamination On the Recipient Sowmentioning

confidence: 99%

Diseases in swine transmitted by artificial insemination: An overview

et al. 2008

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Artificial insemination (AI) of swine is widely practiced in countries with an intensive pig production. It is a very useful tool to introduce superior genes into sow herds, with minimal risk for disease transmission. However, the impact of semen that is contaminated with pathogens can be enormous. Most of the micro-organisms that have been detected in boar semen are considered non-pathogenic, but some are known pathogens (e.g. porcine reproductive and respiratory syndrome virus) that can cause major economic losses. Microbial contamination of semen can be due to systemic and/or urogenital tract infections of the boar, or can occur during collection, processing and storage. It can result in reduced semen quality, embryonic or fetal death, endometritis and systemic infection and/or disease in the recipient female. Conventional techniques for isolation of bacteria and viruses from the semen do not always provide optimal results for various reasons, including lack of sensitivity and speed of testing, and difficult interpretation of the outcome. More recently, PCR tests are commonly used; they have a high sensitivity, the outcome is quickly obtained, and they are suitable for monitoring a large number of samples. The best strategy to prevent AI-transmitted diseases is to use boars that are free of specific pathogens, to monitor the animals and semen regularly, and to maintain very high biosecurity. Additional measures should be directed at treating semen with appropriate antimicrobials, and at reducing contamination during semen collection, processing, and storage. #

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“…The etiological role of PRRSV in reproductive failure of swine is firmly established (Christianson et al, 1993;Lager & Mengeling, 1995;Mengeling et al, 1994;Prieto et al, 1996a;Terpstra et al, 1991). The effect of PRRSV on reproductive parameters was found to be highly related to strain pathogenicity (Prieto et al, 1997). Sows and gilts bred by infected boars (Gradil et al, 1996;Yaeger et al, 1993) or with experimentally contaminated semen (Prieto et al, 1997;Swenson et al, 1994b) typically show seroconversion to PRRSV, even in the absence of detectable viremia (Christopher-Hennings et al, 1995a, 2001.…”

Section: Porcine Reproductive and Respiratory Syndrome Virusmentioning

confidence: 99%

“…Sows and gilts bred by infected boars (Gradil et al, 1996;Yaeger et al, 1993) or with experimentally contaminated semen (Prieto et al, 1997;Swenson et al, 1994b) typically show seroconversion to PRRSV, even in the absence of detectable viremia (Christopher-Hennings et al, 1995a, 2001. It has been reported that insemination of seronegative or pre-immunized gilts with boar semen containing PRRSV often has little to no effect on conception rates, but may result in early embryonic infection and death (Prieto et al, 1997). In addition, PRRSV can be a major cause of prenatal death in commercial swine herds and often preweaning mortality is also increased.…”

Section: Porcine Reproductive and Respiratory Syndrome Virusmentioning

confidence: 99%