Insemination of heifers with sexed frozen or sexed liquid semen

Seidel, G.E.; Cran, D. G.; Herickhoff, Lisa A.; Schenk, J.L.; Doyle, S. P.; Green, R.D

doi:10.1016/s0093-691x(99)91959-3

Cited by 27 publications

(16 citation statements)

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“…Even with high‐speed flow cytometry, the production of sorted spermatozoa with high purity above 90% is limited to 15 million per hour (Rath et al. 2003b) and usually for economic reasons not more than 2 × 10 6 live spermatozoa are used for inseminations (Seidel et al. 1997, 1999a,b; Doyle et al.…”

Section: Discussionmentioning

confidence: 99%

Insemination with Sex Sorted Fresh Bovine Spermatozoa Processed in the Presence of Antioxidative Substances

Klinc

Frese²,

Osmers³

et al. 2007

Reprod Domestic Animals

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Flow cytometrically sex sorted spermatozoa are reduced in their fertilizing capacity, particularly when stored either in cooling extender or after freezing in liquid nitrogen. So far, preservation methods for sorted spermatozoa have differed only marginally from procedures used for unsorted semen. In the present study, a TRIS extender was modified to balance major cell damage caused by the sorting process and by liquid storage of the sorted spermatozoa. The new extender, containing a combination of antioxidants (AO) and bovine serum albumin (BSA), significantly increased the lifespan and fertilizing capacity of sex sorted spermatozoa. No significant differences were observed between unsorted controls and sorted samples for motility and status of sperm membranes as tested by fluorescein-isothiocyanat-peanut agglutinin/propidium iodide (FITC-PNA/PI). Acrosome integrity of spermatozoa was significantly better when semen was stored at 15 degrees C for 24 and 48 h in an extender containing AO with or without BSA as compared with controls (p < 0.05). There were no significant differences, in pregnancy rates of heifers inseminated at a natural oestrus, between unsorted controls (16/24, 66.7%) and both sorted groups (AO + BSA: 18/31, 58.1% and AO-BSA: 12/22, 54.5%). Additionally, it was shown for the first time that artificial insemination (AI) with liquid sexed bull spermatozoa stored for 72 h after sorting can result in pregnancy rates similar to AI with non-sorted semen.

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Section: Discussionmentioning

confidence: 99%

Insemination with Sex Sorted Fresh Bovine Spermatozoa Processed in the Presence of Antioxidative Substances

Klinc

Frese²,

Osmers³

et al. 2007

Reprod Domestic Animals

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“…2004) and frozen‐thawed sorted ram spermatozoa have a reduced lifespan in vitro (Hollinshead et al. 2003b), suggesting that the reduced in vivo fertility after artificial insemination with sorted spermatozoa (bovine: Seidel et al. 1999b; ovine: Hollinshead et al.…”

Section: Discussionmentioning

confidence: 99%

“…Flow cytometric sorting of spermatozoa into X‐ and Y‐chromosome bearing populations has been used in conjunction with artificial insemination (AI) and in vitro fertilisation (IVF) to produce offspring in a number of species (Johnson 2000). Commercialisation of sperm‐sexing technology requires cryopreservation of sex‐sorted spermatozoa for use with AI, which has resulted in the birth of offspring in cattle (Seidel et al. 1999a,b), horses (Lindsey et al.…”

Section: Introductionmentioning

confidence: 99%

Production of Lambs After the Transfer of Fresh and Cryopreserved in vitro Produced Embryos from Prepubertal Lamb Oocytes and Unsorted and Sex‐sorted Frozen‐thawed Spermatozoa

Morton

Catt

Hollinshead

et al. 2004

Reprod Domestic Animals

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Cumulus-oocyte complexes from hormone-stimulated 3-4-week-old (n=43) and 6-7-week-old (n=12) prepubertal lambs were matured in vitro and incubated with unsorted, or X- or Y-spermatozoa separated with a high-speed cell sorter (SX MoFlo)frozen-thawed. Presumptive zygotes were then cultured to the blastocyst stage, and transferred to recipients fresh or after cryopreservation (frozen). Oocyte cleavage was higher (p <0.05) with unsorted (515/926, 55.6%) than X- or Y-spermatozoa (261/672, 38.8% and 229/651, 35.2%, respectively) and blastocyst formation (% zygotes) by Day 9 of in vitro culture was lower (p <0.05) for X- (102/261, 39.1%) than unsorted spermatozoa (249/515, 48.3%), but did not differ between Y-spermatozoa (103/229, 45.0%) and unsorted spermatozoa, or between X- and Y-spermatozoa (p >0.05). For fresh embryos, survival to term was 50.0% (3/6) for unsorted, 0.0% (0/6) for X- and 16.7% (1/6) for Y-spermatozoa-derived embryos (p >0.05), and for frozen embryos was 4.0% (2/50) for unsorted, 9.1% (2/22) for X- and 2.9% (1/34) Y-spermatozoa-derived embryos (p >0.05). Of the two lambs born from X-spermatozoa-derived embryos, one was female (50%), and from the two Y-spermatozoa-derived lambs, both were male (100%), demonstrating that lambs can be produced after the transfer of fresh and cryopreserved IVP embryos derived from prepubertal lamb oocytes and frozen-thawed sex-sorted sperm.

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“…Authors investigating the insemination of sorted sperm in various species have found reduced fertility in cattle [15], pigs [16], and, in early studies, sheep [14]. Moreover, the use of sorted sperm resulted in reduced litter size in the pig [16] and rabbit [3].…”

Section: In Vivo Fertility Of Sorted Spermmentioning

confidence: 99%