Insecticide susceptibility status in two medically important mosquito vectors, Anopheles gambiae, and Culex quinquefasciatus to three insecticides commonly used in Niger State, Nigeria

Shehu, Ibrahim Kura; Olayemi, Israel Kayode; Solomon, Danjuma; Ahmad, Abu Hassan; Salim, Hasber

doi:10.1016/j.sjbs.2022.103524

Cited by 2 publications

(1 citation statement)

References 42 publications

(61 reference statements)

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“…The percentage mortality and KDT 50 range values obtained from the tested insecticide in these study areas (45.829 for Nestle and 44.957 Min for Atan) are vulnerable to prospective resistance as well as multiple and cross-resistance. Previous research showed that mosquito species with high KDT 50 (30.08-59.36) support resistance, whereas those with lower KDT 5O support vulnerable state[17]. Atan and Nestle sites had signi cant levels of deltamethrin insecticide resistance in Anopheles gambiae s.s. together with high allelic frequencies.…”

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confidence: 92%

Deltamethrin Resistance Profiling in Anopheles gambiae: A Study in Ota, Ogun State, Nigeria

Tebamifor,

Mamudu,

Zakari

et al. 2023

Preprint

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Pyrethroids account 89.9% of all vector control insecticide use in African in terms of normal spray coverage This study aims to identify mosquito breeding sites and assess the knockdown resistance (kdr) gene responsible for pyrethroids resistance of Anopheles populations in Ota. Anopheles larvae collected from the Atan and Nestle (6⁰ 40' N, 3⁰ 09' E & 6⁰ 41’N, 3⁰ 9’E) were grown to adulthood in an insectary. Female mosquitoes (2-5days old) not blood-fed were evaluated for sensitivity to 0.05% deltamethrin using the standard method of the World Health Organization. To distinguish the An. gambiae complex related species, rDNA intergenic spacers were amplified using PCR. The kdr- genes were genotyped in Anopheles gambiae females. 438 female mosquitos were gathered in total; Aedes (11.17%), Anopheles (28.8%) and Culex (50.9%) being the most abundant species specimens collected. WHO bioassay for exposure to deltamethrin mortality rate for Anopheles species was 39% (Atan) and 37% (Nestle) respectively. Anopheles mosquitoes were resistant to deltamethrin. Anopheles gambiae was found as the main vector within Ota. The Probit model was utilised to obtain the median knockdown time; KDT50 and KDT95 for both sites (Nestle; KDT50 = 45.829 Min, KDT 95 = 88.854 Min & Atan; KDT 50= 44.957 Min, KDT95 = 93.473 Min). The total frequency of the kdr allele (i.e., kdr-W) resistant individuals was 0.86 in Atan and 0.796 in Nestle site. This data, despite being preliminary, highlights the importance of monitoring An. gambiae numbers in these areas to develop an effective approach for managing pyrethroid and DDT resistance in mosquito control. Susceptibility to pyrethroids can be partially restored when used alongside synergist (e.g Piperonyl butoxide- PBO) suggesting likely involvement of metabolic resistance.

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confidence: 92%

Deltamethrin Resistance Profiling in Anopheles gambiae: A Study in Ota, Ogun State, Nigeria

Tebamifor,

Mamudu,

Zakari

et al. 2023

Preprint

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Susceptibility status and synergistic activity of DDT and Lambda-cyhalothrin on Anopheles gambiae and Aedes aegypti in Delta State, Nigeria

Ojianwuna,

Enwemiwe,

Esiwo

et al. 2024

PLoS ONE

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The detection of insecticide resistance in male mosquitoes has been treated with less importance in monitoring insecticide resistance spread in mosquitoes. There are no studies on the susceptibility and synergistic activity of DDT and lambda-cyhalothrin on male Anopheles gambiae and Aedes aegypti in Delta State, Nigeria. Even though studies have extensively reported resistance in female mosquitoes, the susceptibility of male mosquitoes to insecticide classes should be ascertained. In this study, we tested the susceptibility status and synergistic activity of DDT and Lambda-cyhalothrin on An. gambiae and Ae. aegypti in Delta State, Nigeria, in order to ascertain the level of resistance and knockdown. In addition, we modelled the knockdown time using Probit analysis model. WHO bioassay method was used to expose two days old adult mosquitoes to 4% DDT and 0.05% lambda-cyhalothrin. The results showed that An. gambiae mosquitoes exposed to DDT and lambda-cyhalothrin were confirmed resistant (61% and 53% respectively). However, pre-exposing the resistant mosquito population to piperonyl butoxide (4%) showed an increase in mortality to 90% (possible resistance) in DDT and 98% (susceptible) in lambda-cyhalothrin. Ae. aegypti mosquitoes exposed to DDT were susceptible (98%) while those exposed to lambda-cyhalothrin were confirmed resistant (87%) and this increased to complete mortality (100%) in PBO+lambda-cyhalothrin population. Furthermore, the results showed that the knockdown time (KDT50 and KDT95) in An. gambiae exposed to DDT was 39.5–71.2 minutes and 124.5–146.4 minutes respectively, while that of lambda-cyhalothrin was 33.0–81.8 minutes and 64.0–124.4 minutes respectively. In Ae. aegypti, KDT50 and KDT95 was 23.9 and 61.7minutes for DDT exposure whereas it was 5.6–15.3 minutes and 36.1–72.3 minutes for lambda-cyhalothrin exposure. It can be concluded that male An. gambiae mosquitoes exposed to the insecticides were resistant and the causes may be linked to certain resistant genes in the mosquitoes. The chances of transferring resistance are possible in wild species and molecular-based studies on the resistant gene in male mosquitoes as well as the tendencies of transfer are required to establish this focus.

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Insecticide susceptibility status in two medically important mosquito vectors, Anopheles gambiae, and Culex quinquefasciatus to three insecticides commonly used in Niger State, Nigeria

Cited by 2 publications

References 42 publications

Deltamethrin Resistance Profiling in Anopheles gambiae: A Study in Ota, Ogun State, Nigeria

Deltamethrin Resistance Profiling in Anopheles gambiae: A Study in Ota, Ogun State, Nigeria

Susceptibility status and synergistic activity of DDT and Lambda-cyhalothrin on Anopheles gambiae and Aedes aegypti in Delta State, Nigeria

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