Insecticide resistance status of Aedes aegypti in border areas of Yunnan Province

Abstract

BackgroundAedes aegypti is a main vector of arboviral diseases, principally dengue, chikungunya, and Zika. Insecticides remain the most effective vector control method. Pyrethroid is the main insecticides currently used, and the long‐term use of insecticides can cause mosquitoes to develop knockdown resistance. Studying the mutation sites and genotypes of Ae. aegypti can reveal the mutation characteristics and regional distribution of the kdr gene in the Ae. aegypti population. Testing for a correlation between the mutation rate in various populations and pyrethrin resistance can clarify the resistance mechanism.ResultsThe bioassay results showed that all 15 populations are resistant populations. In the study of the kdr gene, three non‐synonymous mutations were identified in the DNA of F1s from the wild Ae. aegypti population: S989P (TCC‐CCC), V1016G (GTA‐GGA), and F1534C (TTC‐TGC). The mortality rate of various populations was correlated with the mutation rate at the V1016G + F1534C locus but not with that at the S989P + V1016G locus.ConclusionAe. aegypti populations in border areas of Yunnan Province are resistant to permethrin and beta‐cyfluthrin. The insecticidal effect of beta‐cyfluthrin is stronger than that of permethrin. The mutation rate at sites V1016G + F1534C is negatively correlated with the mortality of Ae. aegypti based on bioassay.This article is protected by copyright. All rights reserved.