Insecticide resistance of adults and nymphs of Asian citrus psyllid populations from Apatzingán Valley, Mexico

Pardo, Saúl; Martínez, Amós; Figueroa, José Isaac; Chavarrieta, Juan M.; Viñuela, Elisa; Rebollar-Alviter, Ángel; Miranda, Mario A.; Valle, Javier; Pineda, Samuel

doi:10.1002/ps.4669

Cited by 57 publications

(42 citation statements)

References 22 publications

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“…Resistance to broad-spectrum insecticides in populations of D. citri has been reported in a number of studies from various geographic regions including Pakistan (Naeem et al, 2016), USA (Kanga et al, 2016) and Mexico (Pardo et al, 2018;Vázquez-García et al, 2013). The present study was conducted to determine the toxicity of 10 different insecticides against D. citri collected from citrus groves in Florida.…”

Section: Discussionmentioning

confidence: 98%

“…This approach relies heavily on the use of insecticides to reduce D. citri populations (Grafton‐Cardwell et al., ). However, this reliance on insecticides has led to the development of resistance in D. citri populations in diverse regions (Chen & Stelinski, ; Kanga, Eason, Haseeb, Qureshi, & Stansly, ; Langdon & Rogers, ; Pardo et al., ). Understanding insecticide susceptibility in field populations of D. citri is a critical component of insecticide resistance management (Chen & Stelinski, ).…”

Section: Introductionmentioning

confidence: 99%

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Resistance to commonly used insecticides in Asian citrus psyllid: Stability and relationship to gene expression

Chen

Gill

Ashfaq

et al. 2018

J Applied Entomology

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Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), adults were collected from eight citrus groves across central Florida, and the level of insecticide resistance to ten insecticides was measured using a bottle bioassay. The gene expression of five cytochrome P450 CYP4 (CYP4C67, CYP4DA1, CYP4DB1, CYP4G70 and CYP4C68) and three glutathione S‐transferase (GSTD1, GSTE2 and GSTE1) genes was characterized in seven field populations of D. citri and compared with a laboratory population. Additionally, we reared four neonicotinoid insecticide resistant field populations in the laboratory and observed susceptibility changes without exposure to insecticides over multiple generations. The eight field populations of D. citri adults showed no and very low levels of resistance (RR = 1 and 2–10) to dimethoate, chlorprifos, carbaryl, fenpropathrin, bifenthrin, flupyradifurone and spinetoram. Very low to low resistance was found to imidacloprid and cyantraniliprole (RR = 2–10 and RR = 10–20). Moderate to high resistance was found for thiamethoxam (RR = 20–50 and RR = 50–100). The CYP4G70 and CYP4C68 genes were expressed at a higher level in field populations as compared with the laboratory population. Also the Davenport, Florida field population exhibited higher expression of all target genes compared to the laboratory population. Susceptibility to imidacloprid and thiamethoxam increased by 6.62‐ and 6.42‐fold, respectively, compared to the levels initially observed in the field over six generations of breeding without exposure. These results indicate that insecticide resistance may reverse in the field if insecticide selection pressure is removed from the spray schedule or with use of a rotational scheme with insecticides of different modes of action. Also, the results support use of insecticide resistance survey program combined with effective rotation for integrated insecticide resistance management of D. citri where huanglongbing (HLB) management includes vector suppression with insecticides.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Resistance to commonly used insecticides in Asian citrus psyllid: Stability and relationship to gene expression

Chen

Gill

Ashfaq

et al. 2018

J Applied Entomology

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“…Furthermore, studies of the population source may provide information regarding population characteristics such as basic biology and ecology. Varying levels of susceptibility to different pesticides exist in D. citri populations across citrus‐growing areas worldwide . Some pesticide resistance can be inherited and is associated with the genotype .…”

Section: Discussionmentioning

confidence: 99%

Phylogeography of Diaphorina citri (Hemiptera: Liviidae) and its primary endosymbiont, ‘Candidatus Carsonella ruddii’: an evolutionary approach to host–endosymbiont interaction

Wang

Beattie

Islam

et al. 2018

Pest Management Science

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This study provides evidence for the co-diversification of D. citri and its P-endosymbiont during the migration from South Asia to East and Southeast Asia. We also suggest that the P-endosymbiont may facilitate investigations into the genealogy and migration history of the host. The biogeography of D. citri and its P-endosymbiont indicated that D. citri colonized and underwent a secondary dispersal from South Asia to East and Southeast Asia. © 2018 Society of Chemical Industry.

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“…Unfortunately, these chemical strategies are mostly ineffective due to challenges associated with repetitive and expensive applications and the inability to consistently decrease ACP populations. Growers are also concerned with the development of chemical resistance among ACP populations and the threat long-term chemical applications pose to the environment and beneficial organisms (Tiwari et al, 2011;Hall et al, 2013;Kanga et al, 2016;Pardo et al, 2018). To prevent new citrus plantings from HLB infection and to avoid the development of pesticide-resistant ACP populations, many researchers are targeting control of the vector through biological means (Chen et al, 2018;Tian et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The Detection and Surveillance of Asian Citrus Psyllid (Diaphorina citri)—Associated Viruses in Florida Citrus Groves

et al. 2020

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The plant pathogenic bacterium Candidatus Liberibacter asiaticus (CLas), the causal agent of the citrus disease Huanglongbing (HLB), and its insect vector, the Asian citrus psyllid (ACP; Diaphorina citri), have been devastating the Florida citrus industry. To restore the competitive production presence of Florida in the worldwide citrus market, effective and sustainable control of HLB and the ACP needs to be identified. As alternatives for resistance-inducing insecticides, viruses are currently being considered for biological control of the ACP. To identify possible biological control candidates, we conducted one of the most comprehensive surveys of natural ACP populations in major citrus production regions spanning 21 counties in Florida. By optimizing PCRs and RT-PCRs, we were able to successfully detect and monitor the prevalence of five previously identified ACPassociated RNA and DNA viruses throughout Florida citrus groves, which include: Diaphorina citri-associated C virus (DcACV), Diaphorina citri flavi-like virus (DcFLV), Diaphorina citri densovirus (DcDNV), Diaphorina citri reovirus (DcRV), and Diaphorina citri picorna-like virus (DcPLV). Adult and nymph ACP populations from 21 of Florida's major citrus-producing counties were collected each month during approximately 18 consecutive months. RNA extracts used for these viral screens were also regionally combined and subjected to High Throughput Sequencing (HTS) to reveal a more comprehensive picture of known and unknown viruses in Florida ACP populations. We discovered that DcACV was the most prevalent ACP-associated virus throughout nymph and adult ACP populations in Florida, detected in more than 60% of all samples tested, followed by DcPLV and DcFLV. HTS allowed us to identify a novel ACP-associated reolike virus and a picorna-like virus. The putative reo-like virus, tentatively named Diaphorina citri cimodo-like virus, was later surveyed and detected back in seasonal adult and nymph ACP samples collected in Florida during this study. HTS generated data also revealed that the most abundant virus in Florida ACP populations was Citrus tristeza virus (CTV), which is not an ACP-associated virus, suggesting persistent presence of CTV infection in citrus throughout Florida groves. Collectively, information obtained from our study may be able

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Insecticide resistance of adults and nymphs of Asian citrus psyllid populations from Apatzingán Valley, Mexico

Cited by 57 publications

References 22 publications

Resistance to commonly used insecticides in Asian citrus psyllid: Stability and relationship to gene expression

Resistance to commonly used insecticides in Asian citrus psyllid: Stability and relationship to gene expression

Phylogeography of Diaphorina citri (Hemiptera: Liviidae) and its primary endosymbiont, ‘Candidatus Carsonella ruddii’: an evolutionary approach to host–endosymbiont interaction

The Detection and Surveillance of Asian Citrus Psyllid (Diaphorina citri)—Associated Viruses in Florida Citrus Groves

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