Insecticide resistance in mosquito vectors

Weill, Mylène; Lutfalla, Georges; Mogensen, K. E.; Chandre, Fabrice; Berthomieu, Arnaud; Berticat, Claire; Pasteur, Nicole; Philips, Alexandre; Fort, Philippe; Raymond, M.

doi:10.1038/423136b

Cited by 444 publications

(438 citation statements)

References 4 publications

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“…Lastly, a strong population bottleneck generated by the constant and massive use of insecticides may have occurred. All Tunisian populations indeed carry the G119S resistant allele at the ace-1 resistance locus, except Menzel wherein the additional variants were found (not shown; Weill et al 2003). Guillemaud et al (1997) were marked by grey area, all other crosses being compatible.…”

Section: Discussionmentioning

confidence: 99%

Hypervariable prophage WO sequences describe an unexpected high number of Wolbachia variants in the mosquito Culex pipiens

2005

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Wolbachia are maternally inherited endosymbiotic bacteria that infect many arthropod species and may induce cytoplasmic incompatibility (CI) resulting in abortive embryonic development. Among all the described host species, mosquitoes of the Culex pipiens complex display the highest variability of CI crossing types. Paradoxically, searches for polymorphism in Wolbachia infecting strains and field populations hitherto failed or produced very few markers. Here, we show that an abundant source of the long-sought polymorphism lies in WO prophage sequences present in multiple copies dispersed in the genome of Wolbachia infecting C. pipiens (wPip). We identified up to 66 different Wolbachia variants in C. pipiens strains and field populations and no occurrence of superinfection was observed. At least 49 different Wolbachia occurred in Southern Europe C. pipiens populations, and up to 10 different Wolbachia were even detected in a single population. This is in sharp contrast with North African and Cretan samples, which exhibited only six variants. The WO polymorphism appeared stable over time, and was exclusively transferred maternally. Interestingly, we found that the CI pattern previously described correlates with the variability of Gp15, a prophage protein similar to a bacterial virulence protein. WO prophage sequences thus represent variable markers that now open routes for approaching the molecular basis of CI, the host effects, the structure and dynamics of Wolbachia populations.

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Section: Discussionmentioning

confidence: 99%

Hypervariable prophage WO sequences describe an unexpected high number of Wolbachia variants in the mosquito Culex pipiens

2005

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“…It appears that within the macrolocus, comprising two-third of the proviral segments, large tandem duplications encompassing several segments have played a major role in BV genome expansion [15]. Because duplication boundaries do not correspond to those of the segments [15], it is more likely that a chromosomal mechanism, such as those involved in duplications of insecticide resistance genes [65], rather than a specific viral process, is the basis of these duplications.…”

Section: (I) Gene Expansion Is a Hallmark Of Polydnaviridae Genomesmentioning

confidence: 99%

When parasitic wasps hijacked viruses: genomic and functional evolution of polydnaviruses

Herniou

Huguet

Thézé

et al. 2013

Phil. Trans. R. Soc. B

152

172

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International audienceThe Polydnaviridae (PDV), including the Bracovirus (BV) and Ichnovirus genera, originated from the integration of unrelated viruses in the genomes of two parasitoid wasp lineages, in a remarkable example of convergent evolution. Functionally active PDVs represent the most compelling evolutionary success among endogenous viral elements (EVEs). BV evolved from the domestication by braconid wasps of a nudivirus 100 Ma. The nudivirus genome has become an EVE involved in BV particle production but is not encapsidated. Instead, BV genomes have co-opted virulence genes, used by the wasps to control the immunity and development of their hosts. Gene transfers and duplications have shaped BV genomes, now encoding hundreds of genes. Phylogenomic studies suggest that BVs contribute largely to wasp diversification and adaptation to their hosts. A genome evolution model explains how multidirectional wasp adaptation to different host species could have fostered PDV genome extension. Integrative studies linking ecological data on the wasp to genomic analyses should provide new insights into the adaptive role of particular BV genes. Forthcoming genomic advances should also indicate if the associations between endoparasitoid wasps and symbiotic viruses evolved because of their particularly intimate interactions with their hosts, or if similar domesticated EVEs could be uncovered in other parasites

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“…gambiae, and An. albimanus (Weill et al, 2003(Weill et al, , 2004a), but was not evident among samples of Ae. aegypti representing 28 populations collected worldwide (Weill et al, 2004b).…”

Section: Ace1 Cdnamentioning

confidence: 85%

“…Insensitive AChE-mediated resistance due to nonsynonymous substitutions in the Ace1 gene has since been documented in a number of insects, including the mosquitoes Anopheles gambiae and Cx. pipiens (Alout et al, 2007;Weill et al, 2002Weill et al, , 2003, Anopheles albimanus (Weill et al, 2004a), and Cx. tritaeniorhynchus (Nabeshima et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and characterization of the complete acetylcholinesterase gene (Ace1) from the mosquito Aedes aegypti with implications for comparative genome analysis

Mori

Lobo

deBruyn

et al. 2007

Insect Biochemistry and Molecular Biology

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Insensitive acetylcholinesterase (AChE) has been shown to be responsible for resistance to organophosphates and carbamates in a number of arthropod species. Some arthropod genomes contain a single Ace gene, while others including mosquitoes contain two genes, but only one confers insecticide resistance. Here we report the isolation of the full-length cDNA and characterization of the complete genomic DNA sequence for the Ace1 gene in the yellow fever mosquito, Aedes aegypti. The Ace1 homolog in other mosquito species has been associated with insecticide resistance. The full-length cDNA consists of 2,721 bp and contains a 2,109 bp open reading frame that encodes a 702 amino acid protein. The amino acid sequence is highly conserved with that of other mosquitoes, including greater than 90% identity with Culex spp. and about 80% identity with Anopheles gambiae. The genomic DNA sequence includes 138,970 bp and consists of eight exons with seven introns ranging from 59 to 114,350 bp. Exons 2 and 8 show reduced amino acid conservation across mosquito species, while exons 3-7 are highly conserved. The Ace1 introns in Ae. aegypti reflect a high frequency of repetitive sequences that comprise about 45% of the total intron sequence. The Ace1 locus maps to the p-arm of chromosome 3, which corresponds to the orthologous genome regions in Culex spp. and An. gambiae.

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Insecticide resistance in mosquito vectors

Cited by 444 publications

References 4 publications

Hypervariable prophage WO sequences describe an unexpected high number of Wolbachia variants in the mosquito Culex pipiens

Hypervariable prophage WO sequences describe an unexpected high number of Wolbachia variants in the mosquito Culex pipiens

When parasitic wasps hijacked viruses: genomic and functional evolution of polydnaviruses

Molecular cloning and characterization of the complete acetylcholinesterase gene (Ace1) from the mosquito Aedes aegypti with implications for comparative genome analysis

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