Insecticide-Induced Changes in Protein, RNA, and DNA Contents in Ovary and Fat Body of Female <I>Nilaparvata lugens</I> (Hemiptera: Delphacidae)

Abstract

The widespread use of insecticides is known to cause resurgence in Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) populations, a serious insect pest of rice, Orzya sativa L., crops in Asia. The current study investigated insecticide-induced changes in protein, RNA, and DNA levels in the ovary and fat body of adult female N. lugens. Potted rice plants were sprayed with different concentrations of imidacloprid, triazophos, and detamethrin. The results showed that all three insecticides caused a significant increase in protein and RNA contents in the ovary and fat body of adult females developed from nymphs fed on treated plants compared with those from on untreated plants, although there was no significant effect on DNA content. In general, protein and RNA contents in both ovary and fat body increased linearly with insecticide concentrations or days after emergence (DAEs) when they were applied. There was a significant correlation between protein content and RNA content in both ovary and fat body. In addition, the relationship between ratios of RNA to protein in ovary and fat body of adult female N. lugens and insecticide concentrations showed a significant positive correlation for most DAEs of imidacloprid and several DAEs of triazophos and deltamethrin treatments. These findings suggest that RNA in both ovary and fat body was more sensitive to insecticides and that the insecticide-induced changes in RNA content in turn influence protein synthesis in ovary and fat body. Consistently, RNA-to-protein ratios in ovary and fat body were approximately 0.42 in all treatments and controls.

Adipose triglyceride lipase (Atgl) mediates the antibiotic jinggangmycin-stimulated reproduction in the brown planthopper, Nilaparvata lugens Stål

“…At 2 days after emergence (2 DAE), the adult females were collected to analyze their gene expression profiles. Many previous investigations demonstrated that third instar nymphs are the key stage to pesticide-stimulated reproduction in BPH 10 11 30 .…”

Triazophos‐induced vertical transmission of rice stripe virus is associated with host vitellogenin in the small brown planthopper Laodelphax striatellus

“…In BPH, triazophos also could increase the contents of fat body protein and ovarian protein. 21,22,24,25,30 In addition, exposure to triazophos increased the titers of circulating juvenile hormone, the contents of unfractionated lipids and soluble sugar that supported reproduction of planthoppers. 19 Thus, triazophos was generally considered to be a classic resurgence-inducing insecticide.…”

Inductions of reproduction and population growth in the generalist predator Cyrtorhinus lividipennis (Hemiptera: Miridae) exposed to sub‐lethal concentrations of insecticides

“…The stimulatory effects of sub‐lethal concentrations of triazophos, deltamethrin and imidacloprid have been documented for several rice pests. Previous studies have demonstrated that sub‐lethal concentrations of triazophos (10, 20, 40 and 80 ppm) and deltamethrin (1, 3, 6 and 12 ppm) stimulated the fecundity of N. lugens . A sub‐lethal dose (22.5 g a.i.…”