Insecticidal properties of Solanum nigrum and Armoracia rusticana extracts on reproduction and development of Drosophila melanogaster

Chowański, Szymon; Chudzińska, Ewa; Lelario, Filomena; Ventrella, Emanuela; Marciniak, Paweł; Miądowicz-Kobielska, Mariola; Spochacz, Marta; Szymczak, Monika; Scrano, Laura; Bufo, Sabino Aurelio; Adamski, Zbigniew

doi:10.1016/j.ecoenv.2018.07.030

Cited by 24 publications

(23 citation statements)

References 43 publications

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“…That suggests that the antibiotic may decrease the size of some morphological/anatomical organs. This result was in line with results obtained by Ventrella et al [ 3 ] and Chowański et al [ 4 ], where decreased size and shortened time of development of D. melanogaster exposed to some natural substances was observed. It is possible that the insects speed up their development to the imaginal stage in order to decrease the time of exposure to toxic substances.…”

Section: Discussionsupporting

confidence: 93%

Drosophila melanogaster Response to Feeding with Neomycin-Based Medium Expressed in Fluctuating Asymmetry

Büyükgüzel

Chudzińska

et al. 2020

Insects

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The fruit fly Drosophila melanogaster is a model species used for a wide range of studies. Contamination of Drosophila cultures with bacterial infection is common and is readily eradicated by antibiotics. Neomycin antibiotics can cause stress to D. melanogaster’s larvae and imagoes, which may affect the interpretation of the results of research using culture from neomycin-based medium. In the present study, fluctuating asymmetry (FA), one of the important bioindicators of stress, was measured. Larvae and imagoes of a wild-type D. melanogaster strain were exposed to various concentrations of neomycin. The size of anal papillae and selected wing veins were measured using scanning electron and light microscopy, respectively. Next, the FA was checked. The values obtained for larval anal papillae appeared to be concentration-dependant; the FA indices increased with the concentration of neomycin. The wing FA presented a large but variable correlation, depending on the measured vein. However, the mean length of veins was the highest for the control group, with neomycin-exposed groups showing lower values. The research showed that neomycin may cause sublethal stress in D. melanogaster, which manifests in increased FA indices. This suggests that neomycin can cause physiological and developmental stress in insects, which should be taken into account when interpreting the results of studies using these model organisms.

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Section: Discussionsupporting

confidence: 93%

Drosophila melanogaster Response to Feeding with Neomycin-Based Medium Expressed in Fluctuating Asymmetry

Büyükgüzel

Chudzińska

et al. 2020

Insects

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“…Previous studies have demonstrated that the extract caused larvicidal effects in mosquitoes (Diptera), such as Culex vishnui [17], Culex quinquefasciatus [18], Culex pipiens , Aedes caspius [19], Anopheles culicifacies , Aedes aegypti [20], and Anopheles stephensi [21]. Toxic effects have also been found in the fruitfly ( Drosophila melanogaster : Diptera) [22] and the Colorado potato beetle ( Leptinotarsa decemlineata : Coleoptera) [23]. S. nigrum extract, in addition to its toxic effects, was reported to have promising anticancer [24] and antimicrobial properties [25].…”

Section: Introductionmentioning

confidence: 99%

“…S. nigrum extract, in addition to its toxic effects, was reported to have promising anticancer [24] and antimicrobial properties [25]. The tested extract contains 10 GAs, but two, solasonine and solamargine, are present in greatest amounts [22]. Studies have shown not only the toxic influence of glycoalkaloids on animal health [26], but also the beneficial effects, such as anticancer properties [27,28].…”

Section: Introductionmentioning

confidence: 99%

Sublethal Effects of Solanum nigrum Fruit Extract and Its Pure Glycoalkaloids on the Physiology of Tenebrio molitor (Mealworm)

Spochacz

Chowański

Szymczak

et al. 2018

Toxins

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Background: Solanaceae plants produce glycoalkaloids (GAs) that affect various physiological processes of herbivorous insects and they are being tested as potential alternatives for synthetic pesticides. They cause lethal and sublethal effects. Nevertheless, their mode of action remains unclear. Therefore, we examined the effects of Solanum nigrum fruit extracts and pure glycoalkaloids on a model beetle, Tenebrio molitor. Methods: Plant extracts or pure alkaloids were added to the food of the larvae for three days. The lipid, glycogen, and protein content in the fat body and the midgut were determined, and the contractility of the heart, hindgut, and oviduct muscles was tested using the video-microscopy technique. Finally, the ultrastructure of the fat body and the midgut was observed using electron microscopy. Results: No lethal effects were noted. Sublethal changes were observed in the content of biomolecules, malformations of organelles, chromatin condensation, and heart and oviduct contractility. The observed effects differed between the tested glycoalkaloids and the extract. Conclusions: Both the extract and pure GAs have a wide range of effects that may result in impaired development, food intake, and reproduction. Some early effects may be used as bioindicators of stress. The effects of the extract and pure alkaloids suggest that the substances produced by the plant may act additively or synergistically.

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“…Also, research performed on different insect species showed that different GAs or GAs-containing plant extracts alter the health of individuals. For example, S. nigrum alkaloids and A. rusticana GLSs affected the development and caused imago malformations of model species D. melanogaster [23], whereas tomato and potato GAs and their extracts interrupted the functioning of the fat body cells of the moth Spodoptera exigua [24] and enzymes’ activity in Galleria mellonella moths [25]. Thus far, glycoalkaloids have been considered as potent bioinsecticides and cytotoxic agents rather than therapeutic molecules.…”

Section: Discussionmentioning

confidence: 99%

Differentiated Effects of Secondary Metabolites from Solanaceae and Brassicaceae Plant Families on the Heartbeat of Tenebrio molitor Pupae

Marciniak

Kolińska

Spochacz

et al. 2019

Toxins

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The usage of insects as model organisms is becoming more and more common in toxicological, pharmacological, genetic and biomedical research. Insects, such as fruit flies (Drosophila melanogaster), locusts (Locusta migratoria), stick insects (Baculum extradentatum) or beetles (Tenebrio molitor) are used to assess the effect of different active compounds, as well as to analyse the background and course of certain diseases, including heart disorders. The goal of this study was to assess the influence of secondary metabolites extracted from Solanaceae and Brassicaceae plants: Potato (Solanum tuberosum), tomato (Solanum lycopersicum), black nightshade (Solanum nigrum) and horseradish (Armoracia rusticana), on T. molitor beetle heart contractility in comparison with pure alkaloids. During the in vivo bioassays, the plants glycoalkaloid extracts and pure substances were injected at the concentration 10−5 M into T. molitor pupa and evoked changes in heart activity. Pure glycoalkaloids caused mainly positive chronotropic effects, dependant on heart activity phase during a 24-h period of recording. Moreover, the substances affected the duration of the heart activity phases. Similarly, to the pure glycoalkaloids, the tested extracts also mainly accelerated the heart rhythm, however S. tuberosum and S. lycopersicum extracts slightly decreased the heart contractions frequency in the last 6 h of the recording. Cardioacceleratory activity of only S. lycopersicum extract was higher than single alkaloids whereas S. tubersoum and S. nigrum extracts were less active when compared to pure alkaloids. The most cardioactive substance was chaconine which strongly stimulated heart action during the whole recording after injection. A. rusticana extract which is composed mainly of glucosinolates did not significantly affect the heart contractions. Obtained results showed that glycoalkaloids were much more active than glucosinolates. However, the extracts depending on the plant species might be more or less active than pure substances.

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Insecticidal properties of Solanum nigrum and Armoracia rusticana extracts on reproduction and development of Drosophila melanogaster

Cited by 24 publications

References 43 publications

Drosophila melanogaster Response to Feeding with Neomycin-Based Medium Expressed in Fluctuating Asymmetry

Drosophila melanogaster Response to Feeding with Neomycin-Based Medium Expressed in Fluctuating Asymmetry

Sublethal Effects of Solanum nigrum Fruit Extract and Its Pure Glycoalkaloids on the Physiology of Tenebrio molitor (Mealworm)

Differentiated Effects of Secondary Metabolites from Solanaceae and Brassicaceae Plant Families on the Heartbeat of Tenebrio molitor Pupae

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