Insecticidal effects of Ungernia severtzovii bulb extracts against the grain aphid Schizaphis graminum (Rondani)

Chermenskaya, T. D.; Степанычева, Е. А.; Щеникова, А. В.; Savelieva, Elena; Chakaeva, Anara S.-H.

doi:10.1016/j.indcrop.2011.08.010

Cited by 27 publications

(24 citation statements)

References 17 publications

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“…A 2-cm segment of wheat leaf was dipped into the test solution and placed into each Petri dish. Then, 20 aphids were introduced to the each of prepared Petri dishes [34]. Aphid mortality was measured 24 h after treatment, and the insecticidal efficacy of isolated compounds was calculated by Abbott's formula [35].…”

Section: Insecticidal Activitymentioning

confidence: 99%

Isolation and Bioactivity of Secondary Metabolites from Solid Culture of the Fungus, Alternaria sonchi

et al. 2020

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The fungus, Alternaria sonchi is considered to be a potential agent for the biocontrol of perennial sowthistle (Sonchus arvensis). A new chlorinated xanthone, methyl 8-hydroxy-3-methyl-4-chloro-9-oxo-9H-xanthene-1-carboxylate (1) and a new benzophenone derivative, 5-chloromoniliphenone (2), were isolated together with eleven structurally related compounds (3–13) from the solid culture of the fungus, which is used for the production of bioherbicidal inoculum of A. sonchi. Their structures were determined by spectroscopic (mostly by NMR and MS) methods. Alternethanoxins A and B, which were reported in A. sonchi earlier, were re-identified as moniliphenone and pinselin, respectively. The isolated compounds were tested for phytotoxic, antimicrobial, insecticidal, cytotoxic and esterase-inhibition activities. They did not demonstrate high phytotoxicity (lesions up to 2.5 mm in diameter/length at a concentration of 2 mg/mL) when tested on leaf disks/segments of perennial sowthistle (Sonchus arvensis) and couch grass (Elytrigia repens). They did not possess acute toxicity to Paramecium caudatum, and showed moderate to low cytotoxicity (IC50 > 25 µg/mL) for U937 and K562 tumor cell lines. However, chloromonilicin and methyl 3,8-dihydroxy-6-methyl-4-chloro-9-oxo-9H-xanthene-1-carboxylate (4) were shown to have antimicrobial properties with MIC 0.5–5 µg/disc. Compound 4 and chloromonilinic acid B were found to have contact insecticidal activity to wheat aphid (Schizaphis graminum) at 1 mg/mL. Compounds 2 and methyl 3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate displayed selective carboxylesterase inhibition activity at concentration of 100 µg/mL. Therefore, the waste solid substrate for production of A. sonchi spores can be re-utilized for the isolation of a number of valuable natural products.

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Section: Insecticidal Activitymentioning

confidence: 99%

Isolation and Bioactivity of Secondary Metabolites from Solid Culture of the Fungus, Alternaria sonchi

et al. 2020

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“…Screening candidate plants, purifying active ingredients, isolating and identifying the active plant constituents is required to discover new bioactive natural products [33]. We applied this methodology to identify rosmarinic acid as an active principle from the plant I. rugosus.…”

Section: Discussionmentioning

confidence: 99%

Bioactivity-guided isolation of rosmarinic acid as a principle bioactive compound from the butanol extract of Isodon rugosus against pea aphid, Acyrthosiphon pisum

Khan

Taning

Bonneure

et al. 2019

Preprint

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19Aphids are agricultural pest insects that transmit viruses and cause feeding damage on a global scale. Current pest 20 control involving the excessive use of synthetic insecticides over decades has led to multiple forms of aphid 21 resistance to most classes of insecticides. In nature, plants produce secondary metabolites during their interaction 22 with insects and these metabolites can act as toxicants, antifeedants, anti-oviposition agents and deterrents towards 23 the insects. In a previous study, we demonstrated that the butanol fraction from a crude methanolic extract of an 24 important plant species, Isodon rugosus showed strong insecticidal activity against the pea aphid, Acyrthosiphon 25 pisum. It was however not known as which compound was responsible for such activity. To further explore this 26 finding, current study aimed to exploit a bioactivity-guided strategy to isolate and identify the active compound in 27 the butanol fraction of I. rugosus. As such, reversed-phase flash chromatography, acidic extraction and different 28 spectroscopic techniques were used to isolate and identify the new compound, rosmarinic acid as the bioactive 29 compound in I. rugosus. Insecticidal activity of rosmarinic acid was carried out using standard protocols on A. 30 pisum. The data was analyzed using qualitative and quantitative statistical approaches. Considering that a very low 31 concentration of this compound (LC 90 = 5.4 ppm) causes significant mortality in A. pisum within 24 h, rosmarinic 32 acid could be exploited as a potent insecticide against this important pest insect. Furthermore, I. rugosus is already 33 used for medicinal purposes and rosmarinic acid is known to reduce genotoxic effects induced by chemicals, hence 34 it is expected to be safer compared to the current conventional pesticides. While this study highlights the potential of 35 I. rugosus as a possible biopesticide source against A. pisum, it also provides the basis for further exploration and 36 development of formulations for effective field application. 38Aphids are among the most important agricultural pest insects of many crops worldwide. They feed exclusively on 39 plant phloem sap by inserting their needle-shaped mouthparts into sieve elements, usually resulting to the stunting, 40 discoloration and deformation of plants, while the growth of sooty molds on honeydew produced by these insects 41 reduces the economic value of crops [1, 2]. Moreover, aphids are also vectors of many important plant viruses [3][4][5]. 42The pea aphid, Acyrthosiphon pisum (Hemiptera: Aphididae), adversely affects economically important legume 43 crops worldwide. It is oligophagous, comprising of a number of biotypes or races living on a number of legume 44 hosts (red clover, pea and broad bean and alfalfa races) [6][7][8][9]. Current aphid control strategies predominantly rely on 3 45 the use of insecticides such as carbamates, organophosphates, pyrethroids, neonicotinoids and pymetrozine [10]. 46However, the frequent use of these insecticides over the deca...

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“…This was performed according to the methods of Nong et al. ( 2012 , 2014a ) and Chermenskaya et al. (2012) with some modifications.…”

Section: Methodsmentioning

confidence: 99%

“…We added 2 ml of each concentration to a Petri dish (10 cm in diameter) containing filter paper to absorb the liquid. Ten P. bambucicola aphids were then placed on the filter paper in each Petri dish and incubated at 20 ± 2°C and 80 ± 10% relative humidity ( Chermenskaya et al. 2012 ).…”

Section: Methodsmentioning

confidence: 99%

Aphicidal Activity of an Ageraphorone Extract FromEupatorium adenophorumAgainstPseudoregma bambucicola(Homoptera: Aphididae, Takahashi)

et al. 2015

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The bamboo aphid, Pseudoregma bambucicola, is an important insect pest of bamboo that affects normal bamboo growth and induces sooty molds. The control of P. bambucicola involves the application of chemicals, such as imidacloprid, to which many species are resistant. In this study, we isolate a novel botanical pesticide (9-oxo-10,11-dehydro-ageraphorone) from an Eupatorium adenophorum(Asteraceae: Compositae) petroleum ether extract and test the aphicidal activity of this compound against P. bambucicola in laboratory bioassay and field-based experiments. This ageraphorone compound at a concentration of 2 mg/ml caused 73.33% mortality (corrected mortality [Subtracted the mortality of the negative control]: 70%) of P. bambucicola by laboratory bioassay within 6 h. Even at lower concentrations, this compound caused greater 33% mortality (corrected mortality: 30%) of aphids. Field experiments with naturally infested bamboo plants showed that two applications of 2 mg/ml ageraphorone to infested plants completely cleared infestations within 30 d. These effects were similar to those of the positive control (imidacloprid). These results reveal that 9-oxo-10,11-dehydro-ageraphorone exhibits significant aphicidal activity against bamboo aphids. We suggest that future research be directed at developing this ageraphorone compound from E. adenophorum as an aphicidal agent for biocontrol.

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Insecticidal effects of Ungernia severtzovii bulb extracts against the grain aphid Schizaphis graminum (Rondani)

Cited by 27 publications

References 17 publications

Isolation and Bioactivity of Secondary Metabolites from Solid Culture of the Fungus, Alternaria sonchi

Isolation and Bioactivity of Secondary Metabolites from Solid Culture of the Fungus, Alternaria sonchi

Bioactivity-guided isolation of rosmarinic acid as a principle bioactive compound from the butanol extract of Isodon rugosus against pea aphid, Acyrthosiphon pisum

Aphicidal Activity of an Ageraphorone Extract FromEupatorium adenophorumAgainstPseudoregma bambucicola(Homoptera: Aphididae, Takahashi)

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