Insecticidal Characteristics of Two Commercial <I>Spodoptera exigua</I> Nucleopolyhedrovirus Strains Produced on Different Host Colonies

Abstract

The insecticidal characteristics of two Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) strains produced on two different S. exigua colonies were measured using the same two host colonies. These strains constitute the active ingredients of the biological insecticides Vir-ex and Spexit and were produced on insect colonies from Spain and Switzerland. Demographic characteristics of insects from each colony were examined before infection. Larval developmental time, larval survival, and adult sex ratio did not differ between the colonies, whereas mean pupal weight was significantly higher in the Spanish colony insects. After infection, susceptibility to virus occlusion bodies (OBs), time to death, larval weight at death, and total production of OBs/larva varied significantly depending on virus strain and the colony used. Vir-ex OBs produced in Spanish colony larvae had improved insecticidal characteristics in terms in lethal dose and speed of kill metrics than other strain-colony combinations. OB production was significantly higher in Spanish colony insects infected with Spexit compared with Vir-ex infected insects from the Swiss colony, with intermediate values for the other two strain-colony combinations. Virus strain and host colony origin were highly influential in determining the insecticidal characteristics of OBs and should be considered as key parameters that require optimization during the production of SeMNPV-based insecticides.

Evaluation of Commercial Virus Biopesticides for the Control of Moth Pests in Laboratory Conditions: The Cases of Thaumetopoea pityocampa and Helicoverpa armigera

“…During the last decade, many lab bioassays and field tests have been carried out, evaluating NPVs as biocontrol agents of lepidopteran pests with very promising results. NPVs have demonstrated effective insecticidal action against many notorious moth species, like H. armigera [32], Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) [33], Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) [34][35][36][37], Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) [38,39], Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) [40,41], and Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) [42,43].…”