Insecticidal and repellent action of allyl esters against Acyrthosiphon pisum (Hemiptera: Aphididae) and Tribolium castaneum (Coleoptera: Tenebrionidae)

Giner, Marta; Avilla, J.; Zutter, Nathalie De; Ameye, Maarten; Balcells, Mercè; Smagghe, Guy

doi:10.1016/j.indcrop.2013.02.019

Cited by 17 publications

(9 citation statements)

References 44 publications

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“…These are very low LC values depicted after 24 h of bioassay and such low LC values have not been previously reported in any study with compounds against A . pisum using the same feeding bioassay methodology [30, 37–39, 28, 40, 41]. This means that a very low amount of rosmarinic acid can cause significant toxic effects against A .…”

Section: Discussionmentioning

confidence: 99%

Bioactivity-guided isolation of rosmarinic acid as the principle bioactive compound from the butanol extract of Isodon rugosus against the pea aphid, Acyrthosiphon pisum

et al. 2019

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Aphids are agricultural pest insects that transmit viruses and cause feeding damage on a global scale. Current pest control practices involving the excessive use of synthetic insecticides over many years have resulted in aphid resistance to a number of pesticides. In nature, plants produce secondary metabolites during their interaction with insects and these metabolites can act as toxicants, antifeedants, anti-oviposition agents and deterrents towards the insects. In a previous study, we demonstrated that the butanol fraction from a crude methanolic extract of an important plant species, Isodon rugosus showed strong insecticidal activity against the pea aphid, Acyrthosiphon pisum . To further explore this finding, the current study aimed to exploit a bioactivity-guided strategy to isolate and identify the active compound in the butanol fraction of I . rugosus . As such, reversed-phase flash chromatography, acidic extraction and different spectroscopic techniques were used to isolate and identify the new compound, rosmarinic acid, as the bioactive compound in I . rugosus . Insecticidal potential of rosmarinic acid against A . pisum was evaluated using standard protocols and the data obtained was analyzed using qualitative and quantitative statistical approaches. Considering that a very low concentration of this compound (LC 90 = 5.4 ppm) causes significant mortality in A . pisum within 24 h, rosmarinic acid could be exploited as a potent insecticide against this important pest insect. Furthermore, I . rugosus is already used for medicinal purposes and rosmarinic acid is known to reduce genotoxic effects induced by chemicals, hence it is expected to be safer compared to the current conventional pesticides. While this study highlights the potential of I . rugosus as a possible biopesticide source against A . pisum , it also provides the basis for further exploration and development of formulations for effective field application.

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Section: Discussionmentioning

confidence: 99%

Bioactivity-guided isolation of rosmarinic acid as the principle bioactive compound from the butanol extract of Isodon rugosus against the pea aphid, Acyrthosiphon pisum

et al. 2019

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“…I. rugosus rosmarinic acid gave LC values of LC 50 = 0.2 ppm and LC 90 = 5.4 ppm. These are very low LC values depicted after 24 h of bioassay and such low LC values have not been previously reported in any studies with compounds against A. pisum using the same feeding bioassay methodology [30, 37-39, 28, 40, 41]. This means that a very low amount of rosmarinic acid can cause significant toxic effects against A. pisum in 24 h. Very few insecticidal activities have been reported for rosmarinic acid.…”

Section: Discussionmentioning

confidence: 65%

Bioactivity-guided isolation of rosmarinic acid as a principle bioactive compound from the butanol extract of Isodon rugosus against pea aphid, Acyrthosiphon pisum

Khan

Taning

Bonneure

et al. 2019

Preprint

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19Aphids are agricultural pest insects that transmit viruses and cause feeding damage on a global scale. Current pest 20 control involving the excessive use of synthetic insecticides over decades has led to multiple forms of aphid 21 resistance to most classes of insecticides. In nature, plants produce secondary metabolites during their interaction 22 with insects and these metabolites can act as toxicants, antifeedants, anti-oviposition agents and deterrents towards 23 the insects. In a previous study, we demonstrated that the butanol fraction from a crude methanolic extract of an 24 important plant species, Isodon rugosus showed strong insecticidal activity against the pea aphid, Acyrthosiphon 25 pisum. It was however not known as which compound was responsible for such activity. To further explore this 26 finding, current study aimed to exploit a bioactivity-guided strategy to isolate and identify the active compound in 27 the butanol fraction of I. rugosus. As such, reversed-phase flash chromatography, acidic extraction and different 28 spectroscopic techniques were used to isolate and identify the new compound, rosmarinic acid as the bioactive 29 compound in I. rugosus. Insecticidal activity of rosmarinic acid was carried out using standard protocols on A. 30 pisum. The data was analyzed using qualitative and quantitative statistical approaches. Considering that a very low 31 concentration of this compound (LC 90 = 5.4 ppm) causes significant mortality in A. pisum within 24 h, rosmarinic 32 acid could be exploited as a potent insecticide against this important pest insect. Furthermore, I. rugosus is already 33 used for medicinal purposes and rosmarinic acid is known to reduce genotoxic effects induced by chemicals, hence 34 it is expected to be safer compared to the current conventional pesticides. While this study highlights the potential of 35 I. rugosus as a possible biopesticide source against A. pisum, it also provides the basis for further exploration and 36 development of formulations for effective field application. 38Aphids are among the most important agricultural pest insects of many crops worldwide. They feed exclusively on 39 plant phloem sap by inserting their needle-shaped mouthparts into sieve elements, usually resulting to the stunting, 40 discoloration and deformation of plants, while the growth of sooty molds on honeydew produced by these insects 41 reduces the economic value of crops [1, 2]. Moreover, aphids are also vectors of many important plant viruses [3][4][5]. 42The pea aphid, Acyrthosiphon pisum (Hemiptera: Aphididae), adversely affects economically important legume 43 crops worldwide. It is oligophagous, comprising of a number of biotypes or races living on a number of legume 44 hosts (red clover, pea and broad bean and alfalfa races) [6][7][8][9]. Current aphid control strategies predominantly rely on 3 45 the use of insecticides such as carbamates, organophosphates, pyrethroids, neonicotinoids and pymetrozine [10]. 46However, the frequent use of these insecticides over the deca...

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“…A similar result was obtained by Salari et al (2012), who found that Peganum harmala L. (Tetradiclidaceae) acetonic seed extract acted as a strong repellent on M. persicae. In addition, allyl esters (except allyl hexanoate) had high to moderate repellency to beetles and aphids (Giner et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Contact toxicity was assessed by the method described by Giner et al (2013) with some modifications. In these assays, 0.1 μL pogostone solution at 125, 250, 500, 1,000, 2,000, or 4,000 mg/L was directly applied on the thorax of M. persicae by using a 10 μL syringe, and 0.1 μL acetone was applied as a control.…”

Section: Toxicity Assaymentioning

confidence: 99%

Insecticidal and Repellent Action of Pogostone AgainstMyzus persicae(Hemiptera: Aphididae)

Chen

et al. 2017

Florida Entomologist

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Essential oil of Pogostemon cablin (Blanco) Bentham (Lamiaceae), known as patchouli oil, has been reported to display strong insecticidal activities, but few studies have focused on the insecticidal activity of its principal constituent, pogostone. The present work was designed to evaluate the insecticidal, repellent, and antifeedant activities of pogostone against Myzus persicae (Sulzer) (Hemiptera: Aphididae). In a choice test, pogostone had strong repellent activity against M. persicae. Pogostone also showed pronounced contact toxicity (LC 50 of 1,694 mg/L) to this piercing-sucking insect, as well as powerful antifeedant activity. Pogostone may partly account for the insecticidal activity of patchouli oil, but it also has other valuable properties. This study demonstrated the potential of pogostone as a promising candidate for managing agricultural insects.

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Insecticidal and repellent action of allyl esters against Acyrthosiphon pisum (Hemiptera: Aphididae) and Tribolium castaneum (Coleoptera: Tenebrionidae)

Cited by 17 publications

References 44 publications

Bioactivity-guided isolation of rosmarinic acid as the principle bioactive compound from the butanol extract of Isodon rugosus against the pea aphid, Acyrthosiphon pisum

Bioactivity-guided isolation of rosmarinic acid as the principle bioactive compound from the butanol extract of Isodon rugosus against the pea aphid, Acyrthosiphon pisum

Bioactivity-guided isolation of rosmarinic acid as a principle bioactive compound from the butanol extract of Isodon rugosus against pea aphid, Acyrthosiphon pisum

Insecticidal and Repellent Action of Pogostone AgainstMyzus persicae(Hemiptera: Aphididae)

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