Insect Wing Membrane Topography Is Determined by the Dorsal Wing Epithelium

Belalcazar, Andrea D; Doyle, Kristy; Hogan, Justin; Neff, David; Collier, Simon

doi:10.1534/g3.112.004028

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…In Drosophila, the apical surface of wing epithelial cells changes its morphology, and this morphology acts as a template to produce a rigid dorsal cuticle. After that, a flexible ventral cuticle is produced, which is then molded on the inner surface of the rigid dorsal cuticle [56,57]. A similar mechanism has been proposed in the development of butterfly scales [58].…”

Section: The Induction Model and The Distortion Hypothesismentioning

confidence: 99%

Contact-Mediated Eyespot Color-Pattern Changes in the Peacock Pansy Butterfly: Contributions of Mechanical Force and Extracellular Matrix to Morphogenic Signal Propagation

Otaki¹

2017

Lepidoptera

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Butterfly wing color patterns are developmentally determined by morphogenic signals from organizers in the early pupal stage. However, the precise mechanism of color-pattern determination remains elusive. Here, mechanical and surface disturbances were applied to the pupal hindwing of the peacock pansy butterfly Junonia almana (Linnaeus, 1758) to examine their effects on color-pattern determination. Using the forewing-lift method immediately after pupation, a small stainless ball was placed on the prospective major eyespot or background of the developing dorsal hindwing to cause a wing epithelial distortion, resulting in deformation of the major eyespot. When the exposed dorsal hindwing was covered with a piece of plastic film or placed on a surface of a glass slide, an adhesive tape, or a silicone-coated glassine paper, the major eyespot was effectively reduced in size without a direct contact with the covering materials. The latter two treatments additionally induced the size reduction of the minor eyespot and proximal displacement and broadening of parafocal elements through a direct contact, being reminiscent of the temperatureshock-type modifications. These results suggest the importance of mechanical force and physicochemical properties of planar epithelial contact surface (i.e., extracellular matrix) to propagate morphogenic signals for color-pattern determination in butterfly wings.

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Section: The Induction Model and The Distortion Hypothesismentioning

confidence: 99%

Contact-Mediated Eyespot Color-Pattern Changes in the Peacock Pansy Butterfly: Contributions of Mechanical Force and Extracellular Matrix to Morphogenic Signal Propagation

Otaki¹

2017

Lepidoptera

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“…The integument of insect wings consists of a bilayer of epithelial cells attached at their basal laminae that secrete cuticle from their apical surfaces (Belalcazar et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Extracellular activity of NBAD-synthase is responsible for colouration of brown spots in Ceratitis capitata wings

Pérez

Bochicchio

Rabossi

et al. 2018

Journal of Insect Physiology

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After the emergence of the Ceratitis capitata imago, the pale and folded wings are expanded and sclerotized to acquire the definitive form and to stabilize the cuticle. The wings of this fly show a specific pattern of brownish and black spots. Black spots are pigmented by melanin, whereas there was scarce information about the development of the brownish spots. N-beta-alanydopamine (NBAD) is the main tanning precursor in C. capitata body cuticle, and we hypothesized that it may be responsible for the colouration of the brownish spots. We determined the topology and timing of NBAD synthesis and deposition to attain the species-specific colouration pattern. We demonstrated that during the first hours the colour of the brownish spots was principally determined by the tanning of the hairs. Haemolymph circulation through the veins is required to tan the wings. We confirmed that soon after wing spreading, most of the wing epidermal cells disappeared. Thus, the tanning of the brown spots was accomplished when the wing lamina was devoid of cells. NBAD synthase (NBAD-S; Ebony protein in D. melanogaster) activity in wings was detected in pharate adults and lasted several days after the emergence, even after the end of the tanning process. This observation is in contrast to epidermal NBAD-S activity in the body, where it was nearly undetectable 48 h post emergence. Our results indicate that NBAD-S was exported and deposited into the extracellular matrix of the brown spot areas before cell death and that tanning occurs through gradual export of NBAD precursors (dopamine and b-alanine) from veins.

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Transcriptome Profiling of Neurosensory Perception Genes in Wing Tissue of Two Evolutionary Distant Insect Orders: Diptera (Drosophila melanogaster) and Hemiptera (Acyrthosiphon pisum)

Agnel¹,

Rocha²,

Robichon³

2017

J Mol Evol

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The neurogenesis and neuronal functions in insect wing have been understudied mainly due to technical hindrances that have prevented electrophysiology studies for decades. The reason is that the nano-architecture of the wing chemosensory bristles hampers the receptors accessibility of odorants/tastants to receptors in fixed setup, whereas in nature, the wing flapping mixes these molecules in bristle lymph. In this report, we analyzed the transcriptome of the wing tissue of two species phylogenetically strongly divergent: Drosophila melanogaster a generic model for diptera order (complete metamorphosis) and the aphid acyrthosiphon pisum, representative of hemiptera order (incomplete metamorphosis) for which a conditional winged/wingless polyphenism is under control of population density and resources. The transcriptome shows that extensive gene networks involved in chemosensory perception are active in adult wing for both species. Surprisingly, the specific transcripts of genes that are commonly found in eye were present in Drosophila wing but not in aphid. The analysis reveals that in the aphid conditional wing, expressed genes show strong similarities with those in the gut epithelia. This suggests that the epithelial cell layer between the cuticle sheets is persistent at least in young aphid adult, whereas it disappears after emergence in Drosophila. Despite marked differences between the two transcriptomes, the results highlight the probable universalism of wing chemosensory function in the holometabolous and hemimetabolous orders of winged insects.

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Insect Wing Membrane Topography Is Determined by the Dorsal Wing Epithelium

Cited by 4 publications

References 10 publications

Contact-Mediated Eyespot Color-Pattern Changes in the Peacock Pansy Butterfly: Contributions of Mechanical Force and Extracellular Matrix to Morphogenic Signal Propagation

Contact-Mediated Eyespot Color-Pattern Changes in the Peacock Pansy Butterfly: Contributions of Mechanical Force and Extracellular Matrix to Morphogenic Signal Propagation

Extracellular activity of NBAD-synthase is responsible for colouration of brown spots in Ceratitis capitata wings

Transcriptome Profiling of Neurosensory Perception Genes in Wing Tissue of Two Evolutionary Distant Insect Orders: Diptera (Drosophila melanogaster) and Hemiptera (Acyrthosiphon pisum)

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