Insect transgenesis applied to tephritid pest control

Scolari, Francesca; Schetelig, Marc F.; Gabrieli, Paolo; Siciliano, Paolo; Gomulski, Ludvik M.; Karam, N.; Wimmer, Ernst A.; Malacrida, Anna R.; Gasperi, Giuliano

doi:10.1111/j.1439-0418.2008.01347.x

Cited by 10 publications

(10 citation statements)

References 89 publications

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“…Alternatively, the recent progress of transgenesis allowed development of sexing technologies which have a better potential to be transferred to a wide range of fruit fly species in the genus of Ceratitis, Anastrepha , and Bactrocera [ 26 , 32 - 36 ]. In this case, genetic sexing tools are universally based on a gene transfer system and a sex-specific expression of genes facilitating sex sorting.…”

Section: Introductionmentioning

confidence: 99%

Development of a genetic sexing strain in Bactrocera carambolae (Diptera: Tephritidae) by introgression of sex sorting components from B. dorsalis, Salaya1 strain

et al. 2014

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BackgroundThe carambola fruit fly, Bactrocera carambolae Drew & Hancock is a high profile key pest that is widely distributed in the southwestern ASEAN region. In addition, it has trans-continentally invaded Suriname, where it has been expanding east and southward since 1975. This fruit fly belongs to Bactrocera dorsalis species complex. The development and application of a genetic sexing strain (Salaya1) of B. dorsalis sensu stricto (s.s.) (Hendel) for the sterile insect technique (SIT) has improved the fruit fly control. However, matings between B. dorsalis s.s. and B. carambolae are incompatible, which hinder the application of the Salaya1 strain to control the carambola fruit fly. To solve this problem, we introduced genetic sexing components from the Salaya1 strain into the B. carambolae genome by interspecific hybridization.ResultsMorphological characteristics, mating competitiveness, male pheromone profiles, and genetic relationships revealed consistencies that helped to distinguish Salaya1 and B. carambolae strains. A Y-autosome translocation linking the dominant wild-type allele of white pupae gene and a free autosome carrying a recessive white pupae homologue from the Salaya1 strain were introgressed into the gene pool of B. carambolae. A panel of Y-pseudo-linked microsatellite loci of the Salaya1 strain served as markers for the introgression experiments. This resulted in a newly derived genetic sexing strain called Salaya5, with morphological characteristics corresponding to B. carambolae. The rectal gland pheromone profile of Salaya5 males also contained a distinctive component of B. carambolae. Microsatellite DNA analyses confirmed the close genetic relationships between the Salaya5 strain and wild B. carambolae populations. Further experiments showed that the sterile males of Salaya5 can compete with wild males for mating with wild females in field cage conditions.ConclusionsIntrogression of sex sorting components from the Salaya1 strain to a closely related B. carambolae strain generated a new genetic sexing strain, Salaya5. Morphology-based taxonomic characteristics, distinctive pheromone components, microsatellite DNA markers, genetic relationships, and mating competitiveness provided parental baseline data and validation tools for the new strain. The Salaya5 strain shows a close similarity with those features in the wild B. carambolae strain. In addition, mating competitiveness tests suggested that Salaya5 has a potential to be used in B. carambolae SIT programs based on male-only releases.

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Section: Introductionmentioning

confidence: 99%

Development of a genetic sexing strain in Bactrocera carambolae (Diptera: Tephritidae) by introgression of sex sorting components from B. dorsalis, Salaya1 strain

et al. 2014

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“…Because HID and tTA function not only in Drosophila but also in mammalian systems and mosquitoes, the question of transferability of the Drosophila proof-of-principle [ 57 ] was mostly concerned with the functional conservation of the cis -regulatory control elements of the cellularization genes. A direct transfer of the Drosophila construct using the sry α promoter to drive tTA expression to the Mediterranean fruit fly yielded transgenic flies that did not show any expression of tTA [ 58 ]. This indicates that the cellularization specific sry α promoter from Drosophila is not functional in the Mediterranean fruit fly despite the relative close phylogenetic relationship of these cyclorrhaphan flies.…”

Section: Embryonic Lethality: Transfer To Other Insectsmentioning

confidence: 99%

Transgenic technologies to induce sterility

Catteruccia

Crisanti

Wimmer³

2009

Malar J

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The last few years have witnessed a considerable expansion in the number of tools available to perform molecular and genetic studies on the genome of Anopheles mosquitoes, the vectors of human malaria. As a consequence, knowledge of aspects of the biology of mosquitoes, such as immunity, reproduction and behaviour, that are relevant to their ability to transmit disease is rapidly increasing, and could be translated into concrete benefits for malaria control strategies. Amongst the most important scientific advances, the development of transgenic technologies for Anopheles mosquitoes provides a crucial opportunity to improve current vector control measures or design novel ones. In particular, the use of genetic modification of the mosquito genome could provide for a more effective deployment of the sterile insect technique (SIT) against vector populations in the field. Currently, SIT relies on the release of radiation sterilized males, which compete with wild males for mating with wild females. The induction of sterility in males through the genetic manipulation of the mosquito genome, already achieved in a number of other insect species, could eliminate the need for radiation and increase the efficiency of SIT-based strategies. This paper provides an overview of the mechanisms already in use for inducing sterility by transgenesis in Drosophila and other insects, and speculates on possible ways to apply similar approaches to Anopheles mosquitoes.

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“…In the case of mosquitoes, several trials to control species as Aedes aegypti, Aedes albopictus, Culex pipiens, Culex quinquefasciatus, Anopheles albimanus and Anopheles gambiae were performed, but although many attempts resulted in a reduction in the mosquito population, very few achieved eradication in the release area or long term control (Benedict and Robinson 2003). Transgenic technology may enhance operational SIT programmes at three levels: genetic sexing, sterilization and monitoring (see Scolari et al 2008a for a review). One example of the potential role of transgenesis in implementing pest control is given by RIDL (Release of insects carrying a dominant lethal; Alphey and Andreasen 2002;, a variant of the conventional SIT, in which both genetic sexing and 'sterilization' are achieved by the same construct.…”

Section: Introductionmentioning

confidence: 99%

Safe and fit genetically modified insects for pest control: from lab to field applications

et al. 2010

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Insect transgenesis is continuously being improved to increase the efficacy of population suppression and replacement strategies directed to the control of insect species of economic and sanitary interest. An essential prerequisite for the success of both pest control applications is that the fitness of the transformant individuals is not impaired, so that, once released in the field, they can efficiently compete with or even out-compete their wild-type counterparts for matings in order to reduce the population size, or to spread desirable genes into the target population. Recent research has shown that the production of fit and competitive transformants can now be achieved and that transgenes may not necessarily confer a fitness cost. In this article we review the most recent published results of the fitness assessment of different transgenic insect lines and underline the necessity to fulfill key requirements of ecological safety. Fitness evaluation studies performed in field cages and medium/large-scale rearing will validate the present encouraging laboratory results, giving an indication of the performance of the transgenic insect genotype after release in pest control programmes.

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Insect transgenesis applied to tephritid pest control

Cited by 10 publications

References 89 publications

Development of a genetic sexing strain in Bactrocera carambolae (Diptera: Tephritidae) by introgression of sex sorting components from B. dorsalis, Salaya1 strain

Development of a genetic sexing strain in Bactrocera carambolae (Diptera: Tephritidae) by introgression of sex sorting components from B. dorsalis, Salaya1 strain

Transgenic technologies to induce sterility

Safe and fit genetically modified insects for pest control: from lab to field applications

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