Insect tea attenuates hydrochloric acid and ethanol‑induced mice acute gastric injury

Cheng, Qiang; Qian, Yu; Yi, Ruokun; Gu, Lianjie; Wang, Shanshan; Song, Jia‐Le

doi:10.3892/etm.2017.5181

Cited by 12 publications

(13 citation statements)

References 57 publications

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“…Our results showed that the expression of EGF and EGFR in the gastric tissue of HCl/ethanol-induced gastric injury mice was significantly lower than that of normal mice. EGF, as a protective factor, combines with its receptor EGFR to accelerate ulcer healing, increase tissue repair ability, and maintain ulcer healing quality [46,47]. In the current study, the treatment of HFY09 significantly increased the expression level of EGF and EGFR genes in the gastric tissue of mice with gastric injury, indicating that HFY09 has a protective effect on the gastric tissue of mice with gastric injury, and the effect is stronger at a higher dose.…”

Section: Discussionsupporting

confidence: 53%

Preventive effect of Lactobacillus plantarum HFY09 on HCl/ethanol-induced gastric injury in mice

Sun²,

Ran

et al. 2020

Appl Biol Chem

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The aim of this study was to investigate the effect of Lactobacillus plantarum HFY09 on gastric injury induced by HCl/ ethanol in Kunming mice. The results showed that HFY09-H inhibited any increases in gastric juice volume, maintained the normal pH value of gastric acid, and reduced the damage caused to the gastric mucosa and gastric wall, the inhibition rate on the injury area reaches 63.70%. Compared with the negative control group, HFY09 increased the levels of serum somatostatin (SS) and vasoactive intestinal peptide (VIP), and also decreased the levels of substance P (SP), endothelin-1 (ET-1), interleukin-6 (IL-6), interleukin-12 (IL-12), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ). In addition, real time fluorescent quantitative PCR (Q-PCR) also confirmed that high-dose HFY09 (10 9 CFU/kg/ day) upregulated the mRNA expression of copper/zinc superoxide dismutase (Cu/Zn-SOD), manganese superoxide dismutase (Mn-SOD), catalase (CAT), endothelial nitric oxide synthase (eNOS), and neuronal nitric oxide synthase (nNOS), and downregulated the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). At the same time, the results of the HFY09 treatment group were similar to those of the ranitidine treatment group. These results indicate that HFY09 can prevent gastric injury induced by HCl/ethanol in vivo. Therefore, HFY09 may play a potential role in the treatment of gastric diseases.

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Section: Discussionsupporting

confidence: 53%

Preventive effect of Lactobacillus plantarum HFY09 on HCl/ethanol-induced gastric injury in mice

Sun²,

Ran

et al. 2020

Appl Biol Chem

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“…Nitric oxide, derived from constitutive nitric oxide synthase, is a vital endogenous mediator of mucosal defense and plays a significant role in the maintenance of normal gastric mucosal integrity [54]. This role of NO may be due to its ability to reduce neutrophil infiltration [55] and to influence blood flow in gastric tissues during the healing process of gastric ulcer [56]. In the present study, ethanol ulcerated group showed significant reduction in gastric NO levels in comparing to control group.…”

Section: Discussionmentioning

confidence: 99%

Antiulcerogenic effect of Cuphea ignea extract against ethanol-induced gastric ulcer in rats

Mousa

El-Sammad

Hassan

et al. 2019

BMC Complement Altern Med

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BackgroundCuphea ignea is one of the herbal resources belonging to Lythraceae family. Some species of this family have been used traditionally in South and Central America’s folk medicine for treating stomach disorders. Therefore, the present study was performed to evaluate the gastropreventive effect of aqueous ethanolic extract of C. ignea aerial parts on ethanol-induced gastric ulcer.MethodsGastric ulcers were induced in Sprague Dawley rats using one oral dose of absolute ethanol (1.5 mL/rat). The C. ignea aerial parts extract at doses of 250 and 500 mg/kg body weight and ranitidine (a reference drug) at a dose of 30 mg/kg body weight were orally administrated daily for 7 days before ulcer induction. One hour after ethanol administration blood samples were collected and then stomachs of sacrificed rats were subjected to biochemical, macroscopic and microscopic studies.ResultsOral administration of C. ignea extract significantly attenuated gastric ulcer as revealed by significant reduction in the gastric ulcer index and volume of gastric juice while significantly increased preventive percentage, gastric pH value and pepsin activity. Pre-treatment of C. ignea extract markedly improved the serum level of TNF-α, the gastric MPO activity and NO content. Furthermore, C. ignea pre-treatment significantly increased the gastric levels of enzymatic and non- enzymatic antioxidants namely CAT, SOD, GSH-Px, and GSH with concomitant reduction in MDA level compared with those in the ethanol group. These results were further supported by histopathological findings which revealed the curing effect of C. ignea on the hemorrhagic shock induced by ethanol toxicity.ConclusionsC. ignea extract showed a potential gastroprotective effect on ethanol-induced gastric ulcer, and its effect may be mediated through suppression of oxidative stress and gastric inflammation.

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“…The hepatocytes were then sonicated by SJIALAB for 2 min (10% ultrasound intensity), and centrifuged (4500 rpm) for 10 min to separate the supernatant. The extracted protein was then subjected to polyacrylamide gel electrophoresis (80–120 V), and transferred onto a polyvinylidene fluoride (PVDF) membrane, sealed, and incubated overnight at 4 °C with the corresponding primary antibodies, namely, cyclooxygenase 2 (COX-2; MA514568, Thermo Fisher Scientific), inducible nitric oxide synthase (iNOS) (PA1036), NF-κB (PA1186), phosphorylated (p)-NF-κB p65 (MA515160), nuclear factor of κ-light polypeptide gene enhancer in B-cells inhibitor-α (IκB-α; 397700), Cu/Zn-SOD (PA5270240), Mn-SOD (PA530604), GSH-Px (PA540504), CAT (PA259183), cytochrome P450 reductase (PA577820), and β-actin (MA5157739), incubated at 37 °C with the second antibody (A21241) for 1 h, followed by colorimetric detection and chemiluminescence imaging (iBright FL1000, Thermo Fisher Scientific) [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Polyphenols in Liubao Tea Can Prevent CCl4-Induced Hepatic Damage in Mice through Its Antioxidant Capacities

Pan

Long

et al. 2018

Nutrients

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The present study investigated the preventive effect of polyphenols in Liubao tea (PLT) on carbon tetrachloride (CCl4)-induced liver injury in mice. The mice were initially treated with PLT, followed by induction of liver injury using 10 mL/kg CCl4. Then liver and serum indices, as well as the expression levels of related messenger RNAs (mRNAs) and proteins in liver tissues were measured. The results showed that PLT reduces the liver quality and indices of mice with liver injury. PLT also downregulates aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglycerides (TGs), and malondialdehyde (MDA), and upregulates superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the sera of mice with liver injury. PLT also reduces serum levels of interleukin-6 (IL-6), interleukin-12 (IL-12), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) cytokines in mice with liver injury. Pathological morphological observation also shows that PLT reduces CCl4-induced central venous differentiation of liver tissues and liver cell damage. Furthermore, qPCR and Western blot also confirm that PLT upregulates the mRNA and protein expressions of Gu/Zn-SOD, Mn-SOD, catalase (CAT), GSH-Px, and nuclear factor of κ-light polypeptide gene enhancer in B-cells inhibitor-α (IκB-α) in liver tissues, and downregulates the expression of cyclooxygenase 2 (COX-2) and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB). Meanwhile, PLT also raised the phosphorylated (p)-NF-κB p65 and cytochrome P450 reductase protein expression in liver injury mice. The components of PLT include gallic acid, catechin, caffeine, epicatechin (EC), epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), and epicatechin gallate (ECG), which possibly have a wide range of biological activities. Thus, PLT imparts preventive effects against CCl4-induced liver injury, which is similar to silymarin.

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Insect tea attenuates hydrochloric acid and ethanol‑induced mice acute gastric injury

Cited by 12 publications

References 57 publications

Preventive effect of Lactobacillus plantarum HFY09 on HCl/ethanol-induced gastric injury in mice

Preventive effect of Lactobacillus plantarum HFY09 on HCl/ethanol-induced gastric injury in mice

Antiulcerogenic effect of Cuphea ignea extract against ethanol-induced gastric ulcer in rats

Polyphenols in Liubao Tea Can Prevent CCl4-Induced Hepatic Damage in Mice through Its Antioxidant Capacities

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