Insect-specific viruses regulate vector competence in <i>Aedes aegypti</i> mosquitoes via expression of histone H4

Rp, Olmo; Todjro, Yaovi M H; Aguiar, Eric Roberto Guimarães Rocha; Almeida, João Fernando Lourenço de; Armache, Juliana N.; Faria, Isaque J S de; Fv, Ferreira; Ats, Silva; Kpr, de Souza; App, Vilela; Ch, Tan; Diallo, Mawlouth; Gaye, Alioune; Paupy, Christophe; Obame-Nkoghe, Judicaël; Tm, Visser; Cjm, Koenraadt; Ma, Wongsokarijo; Alc, Cruz; Mt, Prieto; Mcp, Parra; Nogueira, Maurício Lacerda; Avelino-Silva,; Rn, Mota; Maz, Borges; Bp, Drumond; Eg, Kroon; Sedda, Luigi; Marois, Eric; Imler, Jean‐Luc; Jt, Marques

doi:10.1101/2021.06.05.447047

Cited by 6 publications

(19 citation statements)

References 67 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Globally, the Ae. aegypti virome is highly variable in terms of the richness of viral species [22,31]. In the present study, the Ae.…”

Section: Discussionmentioning

confidence: 90%

“…aegypti has a higher capacity to carry viruses and, therefore, to disperse these viruses more widely. This also suggests that this mosquito species is more susceptible to infection by arboviruses such as DENV, ZIKV, and CHIKV [31]. Globally, the Ae.…”

Section: Discussionmentioning

confidence: 99%

“…aegypti, for example, PCLV has been reported to be a predominant ISV in Ae. aegypti populations in Australia [41], Guadeloupe [61], South China [62], Thailand [63], Brazil [31], and Grenada [64]. Interestingly, PCLV is less abundant in African populations such as those in Senegal, and it is absent in Gabon, where the dominant subspecies is related to Ae.…”

Section: Discussionmentioning

confidence: 99%

“…albopictus reveals significant differences in the composition and diversity of ISVs found in these mosquito species. Furthermore, the abundance of some ISVs such as Phasi Charoen-like virus (PCLV) and Humaita-Tubiacanga virus (HTV) may affect the arboviruses infection capacity as well as their transmission dynamics [31].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Local-scale virome depiction supports significant differences between Aedes aegypti and Aedes albopictus

Calle-Tobón

Pérez-Pérez

Forero-Pineda

et al. 2022

Preprint

View full text Add to dashboard Cite

Aedes spp. comprise the primary group of mosquitoes that transmit arboviruses such as dengue, Zika, and chikungunya viruses to humans, and thus these insects pose a significant burden on public health worldwide. Advancements in next-generation sequencing and metagenomics have expanded our knowledge on the richness of RNA viruses harbored by arthropods such as Ae. aegypti and Ae. albopictus ; increasing evidence suggests that vectorial competence can be modified by the microbiome (comprising both bacteriome and virome) of mosquitoes present in endemic zones. Using an RNA-seq-based metataxonomic approach, this study determined the virome structure of field-caught Ae. aegypti and Ae. albopictus mosquitoes in Medellín, Colombia, a municipality with a high incidence of mosquito-transmitted arboviruses. The two species are sympatric, but their core viromes differed considerably in richness, diversity, and abundance; the viromes were dominated by a few viruses. BLAST searches of assembled contigs suggested that at least 17 virus species (16 of which are insect-specific viruses [ISVs]) infect the Ae. aegypti population. Dengue virus 3 was detected in one sample. In Ae. albopictus , up to 11 ISVs and one plant virus were detected. Therefore, the virome composition was species-specific. The bacterial endosymbiont Wolbachia was identified in all Ae. albopictus samples and in some Ae. aegypti samples collected after 2017. The presence of Wolbachi a sp. in Ae. aegypti was not related to significant changes in the richness, diversity, or abundance of this mosquito’s virome, although it was related to an increase in the abundance of Aedes aegypti To virus 2 (unclassified). The mitochondrial diversity of these mosquitoes suggested that the Ae. aegypti population underwent a change that started in the second half of 2017, which coincides with the release of Wolbachia -infected mosquitoes in Medellín, indicating that the population of w Mel-infected mosquitoes has expanded. However, additional studies are required on the dispersal speed and intergenerational stability of w Mel in Medellín and nearby areas as well as on the introgression of genetic variants in the native mosquito population.

show abstract

“…Globally, the Ae. aegypti virome is highly variable in terms of the richness of viral species [22,31]. In the present study, the Ae.…”

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Local-scale virome depiction supports significant differences between Aedes aegypti and Aedes albopictus

Calle-Tobón

Pérez-Pérez

Forero-Pineda

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Local-scale virome depiction in Medellín, Colombia, supports significant differences between Aedes aegypti and Aedes albopictus

et al. 2022

View full text Add to dashboard Cite

Aedes spp. comprise the primary group of mosquitoes that transmit arboviruses such as dengue, Zika, and chikungunya viruses to humans, and thus these insects pose a significant burden on public health worldwide. Advancements in next-generation sequencing and metagenomics have expanded our knowledge on the richness of RNA viruses harbored by arthropods such as Ae. aegypti and Ae. albopictus. Increasing evidence suggests that vector competence can be modified by the microbiome (comprising both bacteriome and virome) of mosquitoes present in endemic zones. Using an RNA-seq-based metataxonomic approach, this study determined the virome structure, Wolbachia presence and mitochondrial diversity of field-caught Ae. aegypti and Ae. albopictus mosquitoes in Medellín, Colombia, a municipality with a high incidence of mosquito-transmitted arboviruses. The two species are sympatric, but their core viromes differed considerably in richness, diversity, and abundance; although the community of viral species identified was large and complex, the viromes were dominated by few virus species. BLAST searches of assembled contigs suggested that at least 17 virus species (16 of which are insect-specific viruses [ISVs]) infect the Ae. aegypti population. Dengue virus 3 was detected in one sample and it was the only pathogenic virus detected. In Ae. albopictus, up to 11 ISVs and one plant virus were detected. Therefore, the virome composition appears to be species-specific. The bacterial endosymbiont Wolbachia was identified in all Ae. albopictus samples and in some Ae. aegypti samples collected after 2017. The presence of Wolbachia sp. in Ae. aegypti was not related to significant changes in the richness, diversity, or abundance of this mosquito’s virome, although it was related to an increase in the abundance of Aedes aegypti To virus 2 (Metaviridae). The mitochondrial diversity of these mosquitoes suggested that the Ae. aegypti population underwent a change that started in the second half of 2017, which coincides with the release of Wolbachia-infected mosquitoes in Medellín, indicating that the population of wMel-infected mosquitoes released has introduced new alleles into the wild Ae. aegypti population of Medellín. However, additional studies are required on the dispersal speed and intergenerational stability of wMel in Medellín and nearby areas as well as on the introgression of genetic variants in the native mosquito population.

show abstract

The virome of the invasive Asian bush mosquitoAedes japonicusin Europe

Abbo

Almeida

Olmo

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Background:The Asian bush mosquitoAedes japonicusis rapidly invading North America and Europe. Due to its potential to transmit multiple pathogenic arthropod-borne (arbo)viruses including Zika virus, West Nile virus and chikungunya virus, it is important to understand the biology of this vector mosquito in more detail. In addition to arboviruses, mosquitoes can also carry insect-specific viruses that receive increasing attention due to their potential effects on host physiology and arbovirus transmission. In this study, we characterized the collection of viruses, referred to as the virome, circulating inAe. japonicuspopulations in the Netherlands and France.Results:Applying a small RNA-based metagenomic approach toAe. japonicus, we uncovered a distinct group of viruses present in samples from both the Netherlands and France. These included one known virus,Ae. japonicus narnavirus 1(AejapNV1), and three new virus species that we namedAe. japonicus totivirus 1(AejapTV1),Ae. japonicus anphevirus 1(AejapAV1) andAe. japonicus bunyavirus 1(AejapBV1). We also discovered sequences that were presumably derived from two additional novel viruses:Ae. japonicus bunyavirus 2(AejapBV2) andAe. japonicus rhabdovirus 1(AejapRV1). All six viruses induced strong RNA interference responses, including the production of 21 nucleotide sized small interfering RNAs, a signature of active replication in the host. Notably, AejapBV1 and AejapBV2 belong to different viral families, however, no RNA-dependent RNA polymerase sequence has been found for AejapBV2. Intriguingly, our small RNA-based approach identified a ~1 kb long ambigrammatic RNA that is associated with AejapNV1 as a secondary segment but showed no similarity to any sequence in public databases. We confirmed the presence of AejapNV1 primary and secondary segments, AejapTV1, AejapAV1 and AejapBV1 by reverse-transcriptase PCR in wild-caughtAe. japonicusmosquitoes. AejapNV1 and AejapTV1 were found at high prevalence (87-100%) in adult females, adult males and larvae.Conclusions:Using a small RNA-based, sequence-independent metagenomic strategy, we uncovered a conserved and prevalent virome amongAe. japonicusmosquito populations. The high prevalence of AejapNV1 and AejapTV1 across all tested mosquito life stages suggests that these viruses are intimately associated withAe. japonicusand may affect different aspects of the physiology of this vector mosquito.

show abstract

Insect-specific viruses regulate vector competence in Aedes aegypti mosquitoes via expression of histone H4

Cited by 6 publications

References 67 publications

Local-scale virome depiction supports significant differences between Aedes aegypti and Aedes albopictus

Local-scale virome depiction supports significant differences between Aedes aegypti and Aedes albopictus

Local-scale virome depiction in Medellín, Colombia, supports significant differences between Aedes aegypti and Aedes albopictus

The virome of the invasive Asian bush mosquitoAedes japonicusin Europe

Contact Info

Product

Resources

About