1991. Pollen morphology and its effect on pollen collection by honey bees, Apis rizellifera L. (Hymenoptera: Apidae), with special reference to upland cotton, Gossjpiruii hirsirfztm L. (Malvaceae). -Gram 33: [128][129][130][131][132][133][134][135][136][137][138]. ISSN 0017-3133. Honey bees, Apis riiellifera, forage readily on flowers of upland cotton, Gossjpirrni /zirsrrtrrrti, to harvest nectar. The abundant pollen gets caught in the haircoat of the bees, but cotton pollen is nevertheless rarely collected. Honey bee pollen collection effectiveness was therefore investigated in a flight room using cotton and five other spheroidal pollen taxa presented in sequence. Honey bees visited all pollen dishes, but ohra pollen (Abcbrroschrrs escirlerzrrrs) was never packed successfully by the bees landing in the pollen dish. Cotton pollen was collected by 16% of the landing foragers, pumpkin pollen (Crtcrrrbifa p e p ) by 7 1%. and pollen of corn (Zen rrrajs), pigweed (Aiiroraritlirrs palnzeri), and sunflower (Heliarithrrs arrrirm) were readily collected by nearly all foragers. The amount of time spent in the pollen dish was always short ( I to 9 seconds) and homogeneous among all pollen taxa, indicating that none of them was strongly repellent to the bees. The reduced effectiveness with which honey bees collected cotton pollen was demonstrated by the longer amount of time needed for pollen grooming and packing between two consecutive landings in the pollen dish and the small size of cotton pollen pellets (averages of 0.42 mg and 8.23 mg per pellet for cotton and corn pollen, respectively). This reduced efficiency in cotton pollen collection was associated primarily with the length of the spines on cotton pollen which physically interfered with the pollen aggregating process used by honey bees. In pollination studies for hybrid cotton seed production, honey bee foragers are often found in greater densities in the male-sterile flowers than in the male-fertile flowers (review in Vaissikre 1991). Several authors (Moffett et al. 1975, Loper 1984, Waller et al. 1985 have attributed this situation to a relative 'repellency' of cotton pollen and the sesquiterpenoid gossypol has been suggested as responsible for this effect (Moffett 1983), but Loper (1986) did not detect any gossypol in cotton pollen. Vansell (1944) suggested that the large size of cotton pollen grains and their covering with 'beads of viscid material' was such that honey bees were unable to pack these grains into their corbiculae. Buchmann
MATERIAL AND METHODSTests were conducted primarily with six spheroidal pollen differing in grain morphology and surface topography ( Fig. 1 ; Table I). Zen iiioys, Heliorzthiis oriiiiiirs, and Aiiioror~rhiis polrircri pollen are readily collected by honey bees in the open, while that of Ciiciirbira pep0 is not often collected and we have never observed nor found a record of honey bees collecting Abebtioschirs pollen though they visit the flowers readily for nectar (Percival 1937(Percival . 1955Rashad & Parker 1958, Hurd 196...