Insect optic lobe neurons identifiable with monoclonal antibodies to GABA

Meyer, Éric P.; Matute, Carlos; Streit, P.; Nässel, Dick R.

doi:10.1007/bf00495784

Cited by 140 publications

(100 citation statements)

References 40 publications

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“…With the antibody used in the present study, i.e. mAb 3A12, selective staining patterns had also been obtained in rats , insects (Meyer et al, 1986), and adult pigeons (Domenici et al, 1988). As shown by the present study, mAb 3A12 was able to reveal GABAir neurons in glutaraldehydefixed pigeon brains during posthatching development.…”

Section: Distribution Of Gaba-li In the Optic Tectum Of Adult Pigeonssupporting

confidence: 72%

Changing distribution of GABA-like immunoreactivity in pigeon visual areas during the early posthatching period and effects of retinal removal on tectal GABAergic systems

et al. 1989

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The distribution of GABA-like immunoreactivity in the pigeon visual system was studied during the first 9 days after hatching using a mouse monoclonal antibody, mAb 3A12, to glutaraldehyde linked GABA . GABA-like immunoreactivity was seen in cell bodies as well as in neuropil at the level of both the retina and central visual regions at any posthatching age. However, the distribution of putative GABAergic cells and processes varied with age reaching the adult pattern at around 9 days. As a general observation, almost no cell bodies in the retina (except for some perikarya in the ganglion cell layer) were labeled at hatching but densely packed immunostained processes were present in the inner plexiform layer. During the next few days, GABA-immunoreactive amacrine and horizontal cells appeared and the adult distribution of GABA-like immunoreactivity was reached at around 9 days. In the other visual regions examined, the general trend in the variation of GABA-like immunoreactivity included: (1) a progressive decrease in the density of immunostained cell bodies and (2) an almost parallel increase in the concentration of stained neuropil. Since in pigeons the adult organization of visual pathways and the final distribution of putative GABAergic systems are reached at around the same age, we suggest the possibility that incoming ganglion cell axons play a role in regulating the distribution of GABA-like immunoreactivity in visual areas. This hypothesis is supported by the fact that the distribution of GABA-like immunoreactivity in the superficial layers of the optic tectum was altered following ablation of the contralateral retina immediately after hatching.

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Section: Distribution Of Gaba-li In the Optic Tectum Of Adult Pigeonssupporting

confidence: 72%

Changing distribution of GABA-like immunoreactivity in pigeon visual areas during the early posthatching period and effects of retinal removal on tectal GABAergic systems

et al. 1989

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“…In the adult fly, we observed widespread, punctate labeling for dVGAT throughout the adult central brain and optic lobes (Fig.5), consistent with previous reports using GABAergic markers in Drosophila and other insects (Brotz and Borst, 1996;Brotz et al, 2001;Enell et al, 2007;Harrison et al, 1996;Meyer et al, 1986;Sinakevitch et al, 2003;Sinakevitch and Strausfeld, 2004;Strausfeld et al, 1995). Labeled structures in the central brain include the subesophageal ganglion (Fig.5A,B, SOG), antennal lobe (Fig.5A, Al) and both the ellipsoid body ( Fig.…”

Section: Dvgat Is Widely Expressed In the Adult Nervous Systemsupporting

confidence: 90%

Mutation of theDrosophilavesicular GABA transporter disrupts visual figure detection

Fei

Chow

Chen

et al. 2010

Journal of Experimental Biology

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SUMMARYThe role of gamma amino butyric acid (GABA) release and inhibitory neurotransmission in regulating most behaviors remains unclear. The vesicular GABA transporter (VGAT) is required for the storage of GABA in synaptic vesicles and provides a potentially useful probe for inhibitory circuits. However, specific pharmacologic agents for VGAT are not available, and VGAT knockout mice are embryonically lethal, thus precluding behavioral studies. We have identified the Drosophila ortholog of the vesicular GABA transporter gene (which we refer to as dVGAT), immunocytologically mapped dVGAT protein expression in the larva and adult and characterized a dVGAT minos mutant allele. dVGAT is embryonically lethal and we do not detect residual dVGAT expression, suggesting that it is either a strong hypomorph or a null. To investigate the function of VGAT and GABA signaling in adult visual flight behavior, we have selectively rescued the dVGAT mutant during development. We show that reduced GABA release does not compromise the active optomotor control of wide-field pattern motion. Conversely, reduced dVGAT expression disrupts normal object tracking and figure-ground discrimination. These results demonstrate that visual behaviors are segregated by the level of GABA signaling in flies, and more generally establish dVGAT as a model to study the contribution of GABA release to other complex behaviors.

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“…These studies have mapped the distribution of enkephalin-like and substance P-like peptides (Mantillas et al,198 1); GABA (Datum et al, 1986;Meyer et al, 1986;Homberg et al, 1987), serotonin (Beltz and Kravitz, 1983;Elofsson, 1983;Nassel et al, 1985), the peptide proctolin (Siwicki and Bishop, 1986), dopamine (Vieillemaringe et al, 1984), choline acetyltransferase (Buchner et al, 1986;Gorczyca and Hall, 1987), and a crustacean peptide hormone (Schueler et al, 1986).…”

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confidence: 99%

Acetylcholine in the crayfish optic lobe: concentration profile and cellular localization

et al. 1989

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The crayfish optic lobe contains high levels of acetylcholine (ACh) and choline as measured with a chemiluminescent assay in small fragments of optic lobe tissue. The highest concentrations were found in the medulla externa and medulla interna (second and third optic neuromeres), which have ACh concentrations of 270 pmol/mg tissue. This concentration is about 16 times that measured in the photoreceptors and lamina ganglionaris (the first optic neuromere). Immunocytochemistry (based upon antisera to choline-glutaryl-BSA) revealed low levels of ACh-like reactivity in the lamina ganglionaris associated with the terminal arbors of centrifugal and/or tangential neurons. The most intense ACh- like reactivity was observed in monopolar neurons of the medulla externa and medulla interna. One monopolar neuron/medullary column (or about 2500 neurons/medullary neuropile) exhibited reactivity and an estimated cytoplasmic concentration of 8.1 mM.

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Insect optic lobe neurons identifiable with monoclonal antibodies to GABA

Cited by 140 publications

References 40 publications

Changing distribution of GABA-like immunoreactivity in pigeon visual areas during the early posthatching period and effects of retinal removal on tectal GABAergic systems

Changing distribution of GABA-like immunoreactivity in pigeon visual areas during the early posthatching period and effects of retinal removal on tectal GABAergic systems

Mutation of theDrosophilavesicular GABA transporter disrupts visual figure detection

Acetylcholine in the crayfish optic lobe: concentration profile and cellular localization

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