Insect juvenile hormone action as a potential target of pest management

Minakuchi, Chieka; Riddiford, Lynn M.

doi:10.1584/jpestics.31.77

Cited by 60 publications

(49 citation statements)

References 130 publications

(133 reference statements)

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“…Furthermore, the dose-response relationships of the tested compounds were comparable to those observed in the induction of the BmKr-h1 transcript in NIAS-Bm-aff3 cells. Given that JHs are insect-specific hormones (41), factors involved in the JH signaling pathway, including the JH receptor, are not likely to be present in mammalian cells. Therefore, it is a reasonable interpretation that BmMet2 accepts JH as a ligand and thereby gains the ability to increase transcription of a gene downstream of the interacting site.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptional regulation of juvenile hormone-mediated induction of Krüppel homolog 1, a repressor of insect metamorphosis

Kayukawa

Minakuchi²,

Namiki³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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274

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The Krüppel homolog 1 gene (Kr-h1) has been proposed to play a key role in the repression of insect metamorphosis. Kr-h1 is assumed to be induced by juvenile hormone (JH) via a JH receptor, methoprene-tolerant (Met), but the mechanism of induction is unclear. To elucidate the molecular mechanism of Kr-h1 induction, we first cloned cDNAs encoding Kr-h1 (BmKr-h1) and Met (BmMet1 and BmMet2) homologs from Bombyx mori. In a B. mori cell line, BmKrh1 was rapidly induced by subnanomolar levels of natural JHs. Reporter assays identified a JH response element (kJHRE), comprising 141 nucleotides, located ∼2 kb upstream from the BmKr-h1 transcription start site. The core region of kJHRE (GGCCTCCACGTG) contains a canonical E-box sequence to which Met, a basic helix-loophelix Per-ARNT-Sim (bHLH-PAS) transcription factor, is likely to bind. In mammalian HEK293 cells, which lack an intrinsic JH receptor, ectopic expression of BmMet2 fused with Gal4DBD induced JHdependent activity of an upstream activation sequence reporter. Meanwhile, the kJHRE reporter was activated JH-dependently in HEK293 cells only when cotransfected with BmMet2 and BmSRC, another bHLH-PAS family member, suggesting that BmMet2 and BmSRC jointly interact with kJHRE. We also found that the interaction between BmMet2 and BmSRC is dependent on JH. Therefore, we propose the following hypothesis for the mechanism of JHmediated induction of BmKr-h1: BmMet2 accepts JH as a ligand, JH-liganded BmMet2 interacts with BmSRC, and the JH/BmMet2/ BmSRC complex activates BmKr-h1 by interacting with kJHRE. development | insecticide | steroid receptor coactivator

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Section: Discussionmentioning

confidence: 99%

“…Because kJHREc and Met/SRC are conserved in other insect species, this mechanism seems to be common in insects. Reporter assays using kJHRE and/or BmMet2 provide a sensitive and efficient screening system for JH agonists and antagonists and may be useful for generating data to develop biorational insecticides (41).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional regulation of juvenile hormone-mediated induction of Krüppel homolog 1, a repressor of insect metamorphosis

Kayukawa

Minakuchi²,

Namiki³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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274

303

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“…It can allow for the combination of pesticides, such as insect growth inhibitors, whose hormonal effects may reduce insect fecundity and fertility, thus reducing reproductive fi tness (Minakuchi & Riddiford 2006, Wimmer et al 2006. Another possible strategy to modify reproductive fi tness could be the use of commercial pesticides mixed with sugar solutions, especially those with hormonal effect similar to the juvenile hormone or ecdysis triggering hormone agonists.…”

Section: Resultsmentioning

confidence: 99%

Adult carbohydrate feeding affects reproduction of Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae)

Jordão¹,

Nakano²,

Janeiro³

2010

Neotrop. entomol.

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-Reproduction of most insects depend on nutrients accumulated during the larval stage, but many lepidopteran species will also depend on nutrients obtained at the adult stage. Feeding at the adult stage allows the intake of carbohydrate and amino acid rich solutions, which may have an effect on the species reproduction and population growth. The objectives of the current study were to characterize the effects of sugar consumption by adults of the potato tuber moth, Phthorimaea operculella (Zeller), on its reproduction. To initially test the food intake by adults, a 10% honey solution or water (control), both containing a liquid dye were offered to adult insects 24h after emergence, and the presence of the dye was observed by analysis of their digestive system. The effects of adult feeding on a 10% honey solution on the reproductive performance of P. operculella were evaluated by assessing the oviposition rate, fecundity and fertility. Adult feeding was proved by the presence of the dye within the digestive system of adults of P. operculella. Although the oviposition rate and fertility were not affected by adult feeding, female fecundity was higher in honey-fed females as compared to the water-fed females.KEY WORDS: Color dye, digestive system, ovarian development, reproductive systemThe potato tuber moth, Phthorimaea operculella (Zeller), is considered one of the main causes of potato losses during harvesting and storage. The pest is present all year round and its larvae damage the leaves, twigs and tubers. It is also a pest to several other cultivated Solanaceae species, such as eggplant, pepper, tobacco and tomato (Filgueira 2003a, b). According to Pratissoli et al (2003), the adults oviposit generally up to ten days after mating, and the adult life cycle may be extended to 43 days, with females surviving for longer periods.Although food resources for reproduction of most insects depend on the nutrients accumulated during the larval stage, many lepidopteran species show feeding habits in the adult stage (Chapman 1998). Food intake at the adult stage allows the utilization of carbohydrate and amino acid rich solutions which may be necessary to complete the development of the reproductive system. In the fi eld, fl ower nectar is the main carbohydrate source, although there may be other sources, such as the extrafl oral nectar and hemipteran honeydew (Romeis & Wäckers 2002). No information on how P. operculella ingests carbohydrate-rich sources is available. It is assumed that, as in other species of the same order, sugar ingestion by adults comes mainly from the nectar of fl owers.Several techniques for insect marking are utilized in ecological studies. They play fundamental roles in fi eld assays, such as dispersion analyses, capture and recapture and in nutritional and phenological studies. These techniques include the use of fl uorescent markers or dyes, which allow easier identifi cation, but in addition they must have the lowest possible infl uence over the performed studies (Hagler & Jackson 2001)...

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“…7) JH mimics such as fenoxycarb, diofenolan, and pyriproxyfen are commercially available. 5,6,8) Compounds such as ethyl 4-[2-(t-butylcarbonyloxy) butoxy] benzoate (ETB), KF-13, and KF-31 ( Fig. 1) were previously reported to possess both JH-like and anti-JH activities against the silkworm Bombyx mori.…”

Section: Introductionmentioning

confidence: 99%

Insecticidal activity and up-regulation of juvenile hormone response genes by juvenile hormone agonists in the western flower thrips, <i>Frankliniella occidentalis</i>

et al. 2015

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Insect juvenile hormone (JH) agonists have been utilized as insect growth regulators for pest management against several pest insect species. We investigated insecticidal activity and the induction ratios of two JH-inducible genes, Krüppel homolog 1 (Kr-h1) and broad (br), using 12 test compounds in the western flower thrips Frankliniella occidentalis (Pergande). In insecticidal assays against propupae to inhibit metamorphosis to the adult, the activities of pyriproxyfen, KF-13, ETB, and KF-31 were potent at 10 mM, while those of the other 8 test chemicals were not. In quantitative RT-PCR, these 4 chemicals up-regulated the transcript levels of Kr-h1 and br, while the induction of transcription by chemicals with lower insecticidal activities was relatively small. Thus, the induction ratios of Kr-h1 and br correlated with insecticidal activity. Assessments of the induction ratios of these genes may be a useful method in the preliminary screening of JH agonists.

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Insect juvenile hormone action as a potential target of pest management

Cited by 60 publications

References 130 publications

Transcriptional regulation of juvenile hormone-mediated induction of Krüppel homolog 1, a repressor of insect metamorphosis

Transcriptional regulation of juvenile hormone-mediated induction of Krüppel homolog 1, a repressor of insect metamorphosis

Adult carbohydrate feeding affects reproduction of Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae)

Insecticidal activity and up-regulation of juvenile hormone response genes by juvenile hormone agonists in the western flower thrips, <i>Frankliniella occidentalis</i>

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