Ins and Outs of Interpreting Lipidomic Results

Bestard-Escalas, Joan; Maimó-Barceló, Albert; Pérez-Romero, Karim; López, Daniel H.; Barceló‐Coblijn, Gwendolyn

doi:10.1016/j.jmb.2019.08.006

Cited by 10 publications

(5 citation statements)

References 205 publications

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“…The purity of the compounds was determined by HPLC (Merck-Hitachi LaChrom D-7000 HPLC-DAD/RI system, column, Aminex HPX-87P; 300x7.8 mm; 60°C; H2O; flow 0.5 mL/min) > 98%. 1 H and 13 C NMR data for known compounds were as reported.…”

Section: Methodsmentioning

confidence: 99%

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Stereoselective synthesis of alkyl pyranosides on a laboratory scale

et al. 2020

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Section: Methodsmentioning

confidence: 99%

“…The real and greater importance of this class of compounds was finally given by lipidomics. Lipidomics unveils the complexity of the lipidome in metabolic diseases [1][2][3][4][5] . Since then, fatty acid derivatives have increasingly come into the focus of scientific interest, and their importance has increased.…”

Section: Introductionmentioning

confidence: 99%

Stereoselective synthesis of alkyl pyranosides on a laboratory scale

et al. 2020

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“…The analysis enabled the detection of more than 190 lipid species belonging to four main lipid categories, which in turn are subdivided into several lipid classes: 1) glycerophospholipids, including phosphatidylcholines (PC), lysophosphatidylcholines (LPC), alkyl-phosphatidylcholines (PC O), phosphatidylethanolamines (PE), lysophosphatidylethanolamines (LPE), alkyl-phosphatidylethanolamines (PE O), and phosphatidylinositols (PI); 2) sphingolipids, including sphingomyelins (SM) and ceramides (Cer); 3) glycerolipids, which includes diglycerides (DG) and triglycerides (TG); 4) sterol lipids, in particular, free cholesterol (FC) and cholesterol esters (CE) (Supplementary Fig. 1) [37]. All results at the level of lipids classes can be found in Supplementary Table 3.…”

Section: DI Cile Infection Impacts Plasma Lipid Compositionmentioning

confidence: 99%

Gut microbiome and plasma lipidome analysis reveals a specific impact of Clostridioides difficile infection on intestinal bacterial communities and sterol metabolism

Arcay,

Barceló-Nicolau,

Suárez-Bode

et al. 2023

Preprint

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Background Gut microbiome studies are helping to understand the mechanisms by which Clostridium difficile infection (CDI) and its recurrences are favoured, as well as recovery from it through different types of treatment. In addition to the impact on microbiome composition and given the metabolic changes occurring at the gut level during CDI, our interest focused on the effects at the local and systemic levels, both during the infection and its treatment, by paying particular attention to bile acid (BA) and cholesterol metabolism due to their close relationship to CDI pathogenesis. A cross-sectional case-control study was performed by obtaining stool and plasma samples from healthy volunteers and patients with CDI diagnosis, primary or recurrent, before and after treatment (antibiotic or faecal material transfer (FMT)) for further analysis. Faecal total DNA was used for sequencing the V3 and V4 variable regions of the 16S rRNA gene. Plasma BAs and lipid profile were established using liquid chromatography-tandem mass spectrometry. Results Specific alterations were observed in the gut microbiota of CDI patients, especially in RCDI, characterized by a loss of microbial richness and diversity and related to a reduction in short-chain acid-producing bacteria and increase in bile salt hydrolase-producing bacteria. FMT-treated patients showed a better restoration of the microbiota than antibiotic-treated patients, resembling that of healthy controls and displaying increased levels of Eubacterium coprostanoligenes, a coprostanol-reducing bacterium. Furthermore, CDI and treated patients exhibited a significant rise in total plasma BA content, particularly in taurine-conjugated BAs. Plasma lipidome analysis revealed a global decline in circulating lipids in CDI patients, with the largest impact on cholesteryl esters. We also identified in CDI patients a specific and consistent decrease in the levels of lipid species containing linoleic acid – an essential fatty acid – which recovered after treatment. Conclusions Analysis of the plasma lipidome reflects the impact of CDI on the gut microbiota and its metabolism, evidencing changes in cholesterol, fatty acid, and BA metabolism that are possibly related to the specific alterations observed in gut microbial communities of CDI patients. Importantly, these results suggest that continued nutritional support after successful treatment may be key to a full recovery.

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“…The systematic application of mass spectrometry (MS) methods to lipid analysis has changed this scenario, and currently, there is a large international effort to standardize aspects associated with lipid analysis, from nomenclature to analytical procedures [ 14 ]. In this already complex scenario, the recent irruption of imaging mass spectrometry techniques has added a new level of difficulty as they have unveiled that the tissue lipid profile is even more specific than was initially envisioned [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…A second challenge is the scarce existing knowledge on lipid metabolism and the implication in pathological situations, which hampers the development of this field at the translational level. Even though at least one gene has been attributed to each of the lipid metabolic pathways, there is a shortage of information as to how any of their isoenzymes work, their specificity, or how any of these enzymes are regulated at both the activity and transcriptional levels [ 15 , 17 ]. Despite these difficulties, there are some successful examples of detection of altered lipid metabolism at the clinical level as the diagnosis of severe metabolic diseases in newborns by detecting aberrant levels of very long polyunsaturated fatty acids, or malnourishment by assessing n-3 fatty acids in plasma.…”

Section: Introductionmentioning

confidence: 99%

Fatty Acid Unsaturation Degree of Plasma Exosomes in Colorectal Cancer Patients: A Promising Biomarker

Bestard-Escalas

Reigada

Reyes

et al. 2021

IJMS

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Even though colorectal cancer (CRC) is one of the most preventable cancers, it is currently one of the deadliest. Worryingly, incidence in people <50 years has increased unexpectedly, and for unknown causes, despite the successful implementation of screening programs in the population aged >50 years. Thus, there is a need to improve early diagnosis detection strategies by identifying more precise biomarkers. In this scenario, the analysis of exosomes is given considerable attention. Previously, we demonstrated the exosome lipidome was able to classify CRC cell lines according to their malignancy. Herein, we investigated the use of the lipidome of plasma extracellular vesicles as a potential source of non-invasive biomarkers for CRC. A plasma exosome-enriched fraction was analyzed from patients undergoing colonoscopic procedure. Patients were divided into a healthy group and four pathological groups (patients with hyperplastic polyps; adenomatous polyps; invasive neoplasia (CRC patients); or hereditary non-polyposis CRC. The results showed a shift from 34:1- to 38:4-containing species in the pathological groups. We demonstrate that the ratio Σ34:1-containing species/Σ38:4-containing species has the potential to discriminate between healthy and pathological patients. Altogether, the results reinforce the utility of plasma exosome lipid fingerprint to provide new non-invasive biomarkers in a clinical context.

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Ins and Outs of Interpreting Lipidomic Results

Cited by 10 publications

References 205 publications

Stereoselective synthesis of alkyl pyranosides on a laboratory scale

Stereoselective synthesis of alkyl pyranosides on a laboratory scale

Gut microbiome and plasma lipidome analysis reveals a specific impact of Clostridioides difficile infection on intestinal bacterial communities and sterol metabolism

Fatty Acid Unsaturation Degree of Plasma Exosomes in Colorectal Cancer Patients: A Promising Biomarker

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