Research-based laboratory courses have been shown to stimulate student interest in science and to improve scientific skills. We describe here a project developed for a semester-long researchbased laboratory course that accompanies a genetics lecture course. The project was designed to allow students to become familiar with the use of bioinformatics tools and molecular biology and genetic approaches while carrying out original research. Students were required to present their hypotheses, experiments, and results in a comprehensive lab report. The lab project concerned the yeast casein kinase 1 (CK1) protein kinase Yck2. CK1 protein kinases are present in all organisms and are well conserved in primary structure. These enzymes display sequence features that differ from other protein kinase subfamilies. Students identified such sequences within the CK1 subfamily, chose a sequence to analyze, used available structural data to determine possible functions for their sequences, and designed mutations within the sequences. After generating the mutant alleles, these were expressed in yeast and tested for function by using two growth assays. The student response to the project was positive, both in terms of knowledge and skills increases and interest in research, and several students are continuing the analysis of mutant alleles as summer projects.
INTRODUCTIONVarious organizations examining science education have concluded that an inquiry-based approach to learning is essential to understanding science as a process (Howard Hughes Medical Institute, 1996; Council on Undergraduate Research, 1997; National Research Council [NRC], 2003). As a consequence, many laboratory courses have shifted away from "cookbook" exercises toward an inquiry-based model, allowing students to feel the self-investment and excitement that comes with discovery of new knowledge (Stukus and Lennox, 2001;Eberhardt et al., 2003;Mitchell and Graziano, 2006). Multiweek, inquiry-or research-based projects have been demonstrated to be an effective means of stimulating student interest and enhancing skills in experimental design and interpretation (Myers and Burgess, 2003;Gammie and Erdeniz, 2004;Howard and Miskowski, 2005;Mitchell and Graziano, 2006;Goyette and DeLuca, 2007). In addition, there is an increasing need to incorporate the use of bioinformatics tools into such projects and into undergraduate biology classes in general (NRC, 2003).Here, we report a semester-long research project that requires students to use bioinformatics tools to design and interpret a molecular biology-based experiment investigating structural determinants of protein kinase activity. Our goals for the course were to allow students to experience the excitement and challenges of original research while increasing their ability to understand and use the tools of the modern molecular biologist. Our specific learning objectives were as follows:1. Students will gain understanding of genetics concepts and molecular biology techniques through using them in the context of a multi-st...