Inositolphosphate glycans and a fucosylated xyloglucan oligosaccharide are accumulated upon <i>Arabidopsis thaliana/ Botrytis cinerea</i> infection

Voxeur, Aline; Sechet, Julien; Vernhettes, Samantha

doi:10.1101/2021.06.25.449971

Cited by 2 publications

(2 citation statements)

References 29 publications

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“…3). The main peaks in the mass spectra of CWEs from Col-0 and fer-4 represented mannose-glucuronic acid-inositol-P [massto-charge ratio (m/z) = 597.107], presumably originating from the hydrolysis of polar heads of glycosyl inositol phosphoryl ceramide lipids (34), along with uronic acid-(hexose) 2 -(pentose) 2 (m/z = 781.225) and uronic acid-(hexose) 2 -(pentose) 3 (m/z = 913.267), potentially originating from rhamnogalacturonan I (Fig. 3A, upper trace).…”

Section: Lignin Modification Combined With Loss Of Function Of Feroni...mentioning

confidence: 99%

FERONIA and wall-associated kinases coordinate defense induced by lignin modification in plant cell walls

Liu

Voxeur

et al. 2023

Sci. Adv.

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Altering the content or composition of the cell wall polymer lignin is a favored approach to valorize lignin toward biomaterial and chemical production in the biorefinery. However, modifying lignin or cellulose in transgenic plants can induce expression of defense responses and negatively affect growth. Through genetic screening for suppressors of defense gene induction in the low lignin ccr1-3 mutant of Arabidopsis thaliana , we found that loss of function of the receptor-like kinase FERONIA, although not restoring growth, affected cell wall remodeling and blocked release of elicitor-active pectic polysaccharides as a result of the ccr1-3 mutation. Loss of function of multiple wall-associated kinases prevented perception of these elicitors. The elicitors are likely heterogeneous, with tri-galacturonic acid the smallest but not necessarily the most active component. Engineering of plant cell walls will require development of ways to bypass endogenous pectin signaling pathways.

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Section: Lignin Modification Combined With Loss Of Function Of Feroni...mentioning

confidence: 99%

FERONIA and wall-associated kinases coordinate defense induced by lignin modification in plant cell walls

Liu

Voxeur

et al. 2023

Sci. Adv.

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“…The number of inositol phosphates with regulatory properties ranges from inositol trisphosphate to inositol pyrophosphates [1][2][3][4][5], and you may ask yourself how many regulatory inositol phosphates need a living cell. Apart from this class of inositol phosphates, inositol is a component of cell surface glycans [6]. Bacteria synthesize di-inositol phosphates using CDP-inositol for the synthesis [7].…”

Section: Introductionmentioning

confidence: 99%

Activated Inositol Phosphate, Substrate for Synthesis of Prostaglandylinositol Cyclic Phosphate (Cyclic PIP)—The Key for the Effectiveness of Inositol-Feeding

Gypakis,

Adelt,

Lemoine

et al. 2024

IJMS

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The natural cyclic AMP antagonist, prostaglandylinositol cyclic phosphate (cyclic PIP), is biosynthesized from prostaglandin E (PGE) and activated inositol phosphate (n-Ins-P), which is synthesized by a particulate rat-liver-enzyme from GTP and a precursor named inositol phosphate (pr-Ins-P), whose 5-ring phosphodiester structure is essential for n-Ins-P synthesis. Aortic myocytes, preincubated with [3H] myo-inositol, synthesize after angiotensin II stimulation (30 s) [3H] pr-Ins-P (65% yield), which is converted to [3H] n-Ins-P and [3H] cyclic PIP. Acid-treated (1 min) [3H] pr-Ins-P co-elutes with inositol (1,4)-bisphosphate in high performance ion chromatography, indicating that pr-Ins-P is inositol (1:2-cyclic,4)-bisphosphate. Incubation of [3H]-GTP with unlabeled pr-Ins-P gave [3H]-guanosine-labeled n-Ins-P. Cyclic PIP synthase binds the inositol (1:2-cyclic)-phosphate part of n-Ins-P to PGE and releases the [3H]-labeled guanosine as [3H]-GDP. Thus, n-Ins-P is most likely guanosine diphospho-4-inositol (1:2-cyclic)-phosphate. Inositol feeding helps patients with metabolic conditions related to insulin resistance, but explanations for this finding are missing. Cyclic PIP appears to be the key for explaining the curative effect of inositol supplementation: (1) inositol is a molecular constituent of cyclic PIP; (2) cyclic PIP triggers many of insulin’s actions intracellularly; and (3) the synthesis of cyclic PIP is decreased in diabetes as shown in rodents.

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Inositolphosphate glycans and a fucosylated xyloglucan oligosaccharide are accumulated upon Arabidopsis thaliana/ Botrytis cinerea infection

Cited by 2 publications

References 29 publications

FERONIA and wall-associated kinases coordinate defense induced by lignin modification in plant cell walls

FERONIA and wall-associated kinases coordinate defense induced by lignin modification in plant cell walls

Activated Inositol Phosphate, Substrate for Synthesis of Prostaglandylinositol Cyclic Phosphate (Cyclic PIP)—The Key for the Effectiveness of Inositol-Feeding

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