Inositol transport proteins

Schneider, Sabine

doi:10.1016/j.febslet.2015.03.012

Cited by 92 publications

(88 citation statements)

References 113 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The predicted targets included disease resistance proteins, such as RPM1 (hop-miR01/hop-miR39) and TMV resistance protein N (hop-miR33/hop-miR41) and several kinases, which are known to be associated with plant defense mechanisms through signaling-related processes [58, 59]. Novel targets also included several functional proteins, such as PPR-containing protein (required for normal plant development) [60], EMF1 protein (required for floral repression during vegetative development) [61], transporter proteins (such as ABC, inositol transporter 1, USO1) [62], SAND protein (endosomal maturation protein) [63], ARC5 protein (plastid division specifically in mesophyll cells) [64], GLABRA2 (trichome differentiation) [65], TT12 (controlling the vacuolar sequestration of flavonoids in the seed coat endothelium) [65]. Candidate targets of three novel miRNA families, including hop-miR05 and hop-miR47, failed to be annotated.…”

Section: Resultsmentioning

confidence: 99%

Identification and characterization of microRNAs in Humulus lupulus using high-throughput sequencing and their response to Citrus bark cracking viroid (CBCVd) infection

et al. 2016

View full text Add to dashboard Cite

BackgroundHop (Humulus lupulus L.) plants are grown primarily for the brewing industry and have been used as a traditional medicinal herb for a long time. Severe hop stunt disease caused by the recently discovered Citrus bark cracking viroid (CBCVd) is one of the most devastating diseases among other viroid infections in hop. MicroRNAs (miRNAs) are a class of non-coding small RNAs that play important roles in gene expression regulation. To identify miRNAs in hop and their response to CBCVd-infection, two small RNA (sRNA) libraries were prepared from healthy and CBCVd-infected hop plants and were investigated by high throughput sequencing.ResultsA total of 67 conserved and 49 novel miRNAs were identified. Among them, 36 conserved and 37 novel miRNAs were found to be differentially recovered in response to CBCVd-infection. A total of 311 potential targets was predicted for conserved and novel miRNAs based on a sequence homology search using hop transcriptome data. The majority of predicted targets significantly belonged to transcriptional factors that may regulate hop leaf, root and cone growth and development. In addition, the identified miRNAs might also play an important roles in other cellular and metabolic processes, such as signal transduction, stress response and other physiological processes, including prenylflavonoid biosynthesis pathways. Quantitative real time PCR analysis of selected targets revealed their negative correlation with their corresponding CBCVd-responsive miRNAs.ConclusionsBased on the results, we concluded that CBCVd-responsive miRNAs modulate several hormone pathways and transcriptional factors that play important roles in the regulation of metabolism, growth and development. These results provide a framework for further analysis of regulatory roles of sRNAs in plant defense mechanism including other hop infecting viroids in particular.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3271-4) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

Identification and characterization of microRNAs in Humulus lupulus using high-throughput sequencing and their response to Citrus bark cracking viroid (CBCVd) infection

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In Trypanosoma cruzi , the etiologic agent of Chagas disease or American trypanosomiasis, myo- inositol transport is facilitated by an energy-dependent, phloridzin-sensitive but cytochalasin B-insensitive system, suggesting that there are at least 2 transporters, a sodium-dependent and a sodium-independent one, that are operational in these parasites (42). Leishmania donovani , the agent of leishmaniasis, transports myo- inositol with a single transporter, L. donovani MIT (LdMIT), with a K m of 0.08 mM (43). Like the fungal transporters, LdMIT is a myo- inositol proton symporter driven by a proton gradient and is expressed at the plasma membrane (22).…”

Section: Discussionmentioning

confidence: 99%

“…Inositol and the management of its levels in the human brain are a focus of investigation for the treatment of neurological disorders like bipolar disease, where it is hypothesized that reduction of cellular signaling via inhibition of myo- inositol biosynthesis or reduction of its cellular concentrations reduces or prevents symptoms (29, 43). Inositol monophosphatase is one putative target for lithium therapy, one of the primary treatments for bipolar disease.…”

Section: Discussionmentioning

confidence: 99%

Functional Characterization of Pneumocystis carinii Inositol Transporter 1

Cushion

Collins

Sesterhenn

et al. 2016

mBio

View full text Add to dashboard Cite

Fungi in the genus Pneumocystis live in the lungs of mammals, where they can cause a fatal pneumonia (PCP [Pneumocystis pneumonia]) in hosts with compromised immune systems. The absence of a continuous in vitro culture system for any species of Pneumocystis has led to limited understanding of these fungi, especially for the discovery of new therapies. We recently reported that Pneumocystis carinii, Pneumocystis murina, and most significantly, Pneumocystis jirovecii lack both enzymes necessary for myo-inositol biosynthesis but contain genes with homologies to fungal myo-inositol transporters. Since myo-inositol is essential for eukaryotic viability, the primary transporter, ITR1, was functionally and structurally characterized in P. carinii. The predicted structure of P. carinii ITR1 (PcITR1) contained 12 transmembrane alpha-helices with intracellular C and N termini, consistent with other inositol transporters. The apparent Km was 0.94 ± 0.08 (mean ± standard deviation), suggesting that myo-inositol transport in P. carinii is likely through a low-affinity, highly selective transport system, as no other sugars or inositol stereoisomers were significant competitive inhibitors. Glucose transport was shown to use a different transport system. The myo-inositol transport was distinct from mammalian transporters, as it was not sodium dependent and was cytochalasin B resistant. Inositol transport in these fungi offers an attractive new drug target because of the reliance of the fungi on its transport, clear differences between the mammalian and fungal transporters, and the ability of the host to both synthesize and transport this critical nutrient, predicting low toxicity of potential inhibitors to the fungal transporter.

show abstract

“…In contrast, the apical surfaces of the epithelial cells lining the external body tissues, including the skin, fin, gill, and upper regions of the gastrointestinal tract are faced with much greater osmotic challenges as the external osmolality of <5 mosmol/kg H 2 O found in FW is increased to over 1,000 mosmol/kg H 2 O when fish move to SW. As might be expected, epithelial cells in these tissues must accumulate even higher concentrations of organic osmolytes, including inositol, to maintain their cell volume and, consequently, the integrity of the epithelium. Cellular accumulation of inositol can either be the result of secondary active transport across the plasma membrane by the actions of carriers, such as the sodium myo-inositol transporter (SMIT) and/or the proton myo-inositol transporter (HMIT), as has been reported for various mammalian tissues, including the kidney and brain (6, 12, 28, 41), or can be the result of de novo synthesis from glucose 6-phosphate (36, 43). In the mammalian studies so far reported, neuronal tissues appear to accumulate relatively high concentrations of free inositol (20, 46, 47); however, the brain generally expresses relatively low levels of MIPS mRNA (19), suggesting accumulation via SMIT/HMIT may be more physiologically important than de novo synthesis.…”

Section: Discussionmentioning

confidence: 99%

“…IMPA can dephosphorylate both the inositol 3-phosphate that is generated from MIPS activity and also any inositol 1-phosphate that is produced from cell signaling and the turnover of membrane inositol phospholipids (36). Alternatively, in animal cells, the cyclic alcohol can be sequestered as the result of the actions of two types of membrane transporter, the sodium- and the proton-dependent myo-inositol transporters (abbreviated as SMIT and HMIT, respectively) (41), which use the transmembrane sodium or proton gradients to accumulate the metabolite to concentrations of up to 1,000 times that found in the extracellular environment. Adaptation of euryhaline fish to SW or hyper-saline environments has been reported to result in an increase in expression of IMPA in a number of tissues from the eel (24, 25), killifish (45), and tilapia (15, 24, 25, 39, 44).…”

mentioning

confidence: 99%

Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus): effect of seawater acclimation

Kalujnaia

Hazon

Cramb

2016

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

A single MIPS gene (Isyna1/Ino1) exists in eel and tilapia genomes with a single myo-d-inositol 3-phosphate synthase (MIPS) transcript identified in all eel tissues, although two MIPS spliced variants [termed MIPS(s) and MIPS(l)] are found in all tilapia tissues. The larger tilapia transcript [MIPS(l)] results from the inclusion of the 87-nucleotide intron between exons 5 and 6 in the genomic sequence. In most tilapia tissues, the MIPS(s) transcript exhibits much higher abundance (generally >10-fold) with the exception of white skeletal muscle and oocytes, in which the MIPS(l) transcript predominates. SW acclimation resulted in large (6- to 32-fold) increases in mRNA expression for both MIPS(s) and MIPS(l) in all tilapia tissues tested, whereas in the eel, changes in expression were limited to a more modest 2.5-fold increase and only in the kidney. Western blots identified a number of species- and tissue-specific immunoreactive MIPS proteins ranging from 40 to 67 kDa molecular weight. SW acclimation failed to affect the abundance of any immunoreactive protein in any tissue tested from the eel. However, a major 67-kDa immunoreactive protein (presumed to be MIPS) found in tilapia tissues exhibited 11- and 54-fold increases in expression in gill and fin samples from SW-acclimated fish. Immunohistochemical investigations revealed specific immunoreactivity in the gill, fin, skin, and intestine taken from only SW-acclimated tilapia. Immunofluorescence indicated that MIPS was expressed within gill chondrocytes and epithelial cells of the primary filaments, basal epithelial cell layers of the skin and fin, the cytosol of columnar intestinal epithelial and mucous cells, as well as unknown entero-endocrine-like cells.

show abstract

Inositol transport proteins

Cited by 92 publications

References 113 publications

Identification and characterization of microRNAs in Humulus lupulus using high-throughput sequencing and their response to Citrus bark cracking viroid (CBCVd) infection

Identification and characterization of microRNAs in Humulus lupulus using high-throughput sequencing and their response to Citrus bark cracking viroid (CBCVd) infection

Functional Characterization of Pneumocystis carinii Inositol Transporter 1

Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus): effect of seawater acclimation

Contact Info

Product

Resources

About