Inositol phospholipid hydrolysis in human brain; adenosine inhibition of the response to histamine

Kendall, David A.; Firth, J. L.

doi:10.1111/j.1476-5381.1990.tb12048.x

Cited by 18 publications

(4 citation statements)

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“…H2-receptors and a potentiation of that response by Hrreceptors (Baudry et al, 1975). We have previously shown that phospholipase C-linked HI-receptors are active in human brain slices (Kendall & Firth, 1990) and, as demonstrated in this study, HI-receptors potentiate the effects of P-adrenoceptor activation, so the ineffectiveness of histamine alone is probably due to a low density of directly coupled H2-receptors in human cortex.…”

Section: Discussionsupporting

confidence: 74%

“…The response to isoprenaline was also potentiated by histamine acting, on the evidence of the affinity for mepyramine, through an HI-receptor. The IC50 for this effect of mepyramine (35 nM) was very similar to that found previously for the inhibition of histamine-stimulated phosphoinositide hydrolysis in human brain (Kendall & Firth, 1990) and it is possible that the same HI receptors are involved. This particular example of potentiation would appear to be unique to human brain, at least amongst those species examined to date, and might simply reflect the colocation of P-adrenoceptors and HI-receptors.…”

Section: I-t-l-supporting

confidence: 84%

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Direct and indirect stimulations of cyclic AMP formation in human brain

Kendall

Millns

Firth

1992

British J Pharmacology

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1 Adenosine 3':5'-cyclic monophosphate (cyclic AMP) formation, by use of an [3H]-adenine prelabelling assay, was measured in fragments of human cerebral cortex, taken in the course of various neurosurgical procedures. 2 Large accumulations of [3H]-cyclic AMP due to isoprenaline, noradrenaline and adenosine and small effects due to forskolin were observed. Histamine, 5-hydroxytryptamine (5-HT) and the excitatory amino acids glutamate and quisqualate were ineffective.3 The response to noradrenaline consisted of two components; a direct P-adrenoceptor response and an enhancement mediated by an a-adrenoceptor which appears to be similar to that in rat cerebral cortex.4 The response to isoprenaline was also potentiated by histamine H, receptor stimulation but the direct effect of 2-chloroadenosine was not altered by histamine, 5-HT or quisqualate. 5 It is concluded that some, but not all, of the indirect modulations of cyclic AMP formation previously observed in experimental animal brain exist in human cerebral cortex.

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Section: Discussionsupporting

confidence: 74%

Section: I-t-l-supporting

confidence: 84%

Direct and indirect stimulations of cyclic AMP formation in human brain

Kendall

Millns

Firth

1992

British J Pharmacology

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“…However, it is clear that not all brain tissues show the same very marked dependence on extracellular Ca2+ as mouse and rat cerebral cortex and the Ca2`-dependence observed in the U373 MG cells is much closer to that in the guinea-pig tissues. There is as yet no indication whether this is also the pattern in human cerebral cortex, a tissue in which histamine is known to stimulate phosphoinositide hydrolysis (Kendall & Firth, 1990) and it would be unwise to make predictions on the basis of the response in a transformed cell line.…”

Section: Discussionmentioning

confidence: 99%

Calcium‐dependence of histamine‐ and carbachol‐induced inositol phosphate formation in human U373 MG astrocytoma cells: comparison with HeLa cells and brain slices

Arias‐Montaño

Berger

Young

1994

British J Pharmacology

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Histamine (1 mm) induced an accumulation of inositol monophosphate ([3H]‐IP1) in the U373 MG human astrocytoma cell line which increased with time in the presence of 30 mm Li+. After a 30 min incubation period with 1 mm histamine [3H]‐IP1 was the major product detected (84 ± 1 % of total [3H]‐IPx) and was present at a level 11 (±1) fold of basal accumulation. Concentration‐response curves for histamine‐induced [3H]‐IP1 accumulation in U373 MG cells (EC50 5.4 ± 0.5 μm) were shifted to the right in a parallel fashion by mepyramine (slope of a Schild plot 0.99 ± 0.08), yielding a Kd for mepyramine of 3.5 ± 0.3 nm, consistent with the involvement of histamine H1‐receptors. The temelastine‐sensitive binding of [3H]‐mepyramine to a membrane fraction from U373 MG cells was hyperbolic and had a mean Kd of 2.5 ± 1.0 nm. The maximum amount of temelastine‐sensitive binding was 86 ± 19 pmol g−1 membrane protein. Carbachol also induced [3H]‐IP1 accumulation in U373 MG cells, 2.8 (± 0.1) fold of basal with 1 mm carbachol, with an EC50 of 48 ± 8 μm. Pirenzepine shifted carbachol concentration‐response curves to the right (slope of Schild plot 0.89 ± 0.07) giving a Kd for pirenzepine of 0.10 ± 0.01 μm, suggesting that phosphoinositide hydrolysis in U373 MG cells is mediated by the M3‐, rather than the M1‐, muscarinic receptor subtype. [3H]‐IP1 accumulation induced by both 1 mm histamine and by 1 mm carbachol increased when the Ca2+ concentration of the medium was increased from ‘zero’ (no added Ca2+) to 0.3 mm. Histamine‐stimulated [3H]‐IP1 accumulation was further increased, although not so markedly, as the Ca2+ was raised to 4 mm. The same pattern was apparent with histamine‐induced accumulations of [3H]‐IP2 and [3H]‐IP3. In contrast, [3H]‐IPx accumulation in response to carbachol increased between 0.3 and 1.3 mm, but thereafter remained unchanged ([3H]‐IP1) or declined ([3H]‐IP2 and [3H]‐IP3). In HeLa cells, [3H]‐IP1 accumulations induced by 1 mm histamine and 1 mm carbachol showed the same pattern of Ca2+ dependence and were independent of extracellular Ca2+ above 0.3 mm (histamine) or 1.3 mm (carbachol). The response to carbachol appeared to be mediated by an M3‐muscarinic receptor (apparent Kd for pirenzepine 0.09 μm). In cross‐chopped slices of guinea‐pig cerebral cortex and guinea‐pig cerebellum, [3H]‐IP1 accumulation induced by 1 mm histamine in the presence of 10 mm Li+ increased as the extracellular Ca2+ was increased from 0.3 to 2.5 mm, but a further increase to 4 mm had no further effect. In contrast the response to histamine in rat cerebral cortex increased markedly between 1.3 and 4 mm Ca2+. Accumulations of [3H]‐IP1 induced by carbachol in guinea‐pig or rat cerebral cortical slices were not increased as extracellular Ca2+ was raised from 0.3 to 4 mm. Nimodipine (100 nm) and ω‐conotoxin (3 μm) had no significant effect on histamine‐induced [3H]‐IP1 accumulation in rat cerebral cortical slices or in U373 MG cells. We conclude that histamine‐induced [3H]‐IP1 accumulation in U373 MG cells does appear to have a compo...

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“…In contrast, adenosine inhibited norepinephrine-enhanced phosphoinositidc hydrolysis in rat aorta (Long and Stone, 1987). Further, in cerebral cortex preparations, histamine-, but not norepinephrine-or carbamylchol me-, stimulated phosphoinositide tumover was either augmented or attenuated by adenosinereceptor activation (Hollingsworth et al, 1986;Hill and Kendall, 1987;Kendall and Hill, 1988;Alexander et al, 1989;Kendall and Firth, 1990).…”

Section: Discussionmentioning

confidence: 99%

Activation of Adenosine A₂ Receptors Stimulates Phosphoinositide Metabolism in Rat Peripheral Nerve

Sheldon

Bonasera

Eichberg

1996

Journal of Neurochemistry

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Inositol phospholipid hydrolysis in human brain; adenosine inhibition of the response to histamine

Cited by 18 publications

References 14 publications

Direct and indirect stimulations of cyclic AMP formation in human brain

Direct and indirect stimulations of cyclic AMP formation in human brain

Calcium‐dependence of histamine‐ and carbachol‐induced inositol phosphate formation in human U373 MG astrocytoma cells: comparison with HeLa cells and brain slices

Activation of Adenosine A₂ Receptors Stimulates Phosphoinositide Metabolism in Rat Peripheral Nerve

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Inositol phospholipid hydrolysis in human brain; adenosine inhibition of the response to histamine

Cited by 18 publications

References 14 publications

Direct and indirect stimulations of cyclic AMP formation in human brain

Direct and indirect stimulations of cyclic AMP formation in human brain

Calcium‐dependence of histamine‐ and carbachol‐induced inositol phosphate formation in human U373 MG astrocytoma cells: comparison with HeLa cells and brain slices

Activation of Adenosine A2 Receptors Stimulates Phosphoinositide Metabolism in Rat Peripheral Nerve

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Activation of Adenosine A₂ Receptors Stimulates Phosphoinositide Metabolism in Rat Peripheral Nerve