Inositol phosphates in growing and differentiating HL60 cells

French, Philip J.; Bunce, Christopher M.; Brown, G. R.; Creba, Judith A.; Michell, Robert H.

doi:10.1042/bst0160985

Cited by 18 publications

(11 citation statements)

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“…Therefore, our data for the complexation behaviour of Ins(1,3,4,5,6) P 5 in solution, together with the known concentrations of major cations in cytosol/nucleus, can be used for predicting the probable major form(s) of the compound in living cells. Solubility constants are not needed for these calculations because the data presented below indicate that Ins(1,3,4,5,6) P 5 cannot be expected to precipitate at its reported concentration range in mammalian cells of approximately 10–100 μM [ 28 – 30 ] (a different situation arises with certain non-mammalian erythrocytes, as further discussed below). For the calculations, we chose 50 μM Ins(1,3,4,5,6) P 5 , 150 mM K + and pH 7.4 [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

The behaviour of inositol 1,3,4,5,6-pentakisphosphate in the presence of the major biological metal cations

et al. 2009

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Section: Resultsmentioning

confidence: 99%

The behaviour of inositol 1,3,4,5,6-pentakisphosphate in the presence of the major biological metal cations

et al. 2009

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“…Although these studies have identified many of the same compounds, inositol phosphates other than those downstream of Ins(1,4,5)P3 were much more prominent components of the avian cells, emphasizing the need for similarly detailed studies of other cells. This need is further reinforced by our own similar studies of equilibrium-labelled HL60 promyelocytic cells, which have revealed both a greater variety of inositol phosphates than in WRK1 cells and the presence of some labelled and as yet unidentified 'inositol polyphosphate' peaks (French et al, 1988(French et al, , 1991 …”

Section: Concluding Commentmentioning

confidence: 89%

“…Berridge & Irvine, 1989) and some inositol polyphosphates are among the most abundant polyanions in many cells (e.g. French et al, 1988French et al, , 1991reviewed by Shears, 1989a). It therefore remains disturbing that only two studies, one of normal avian erythrocytes (Stephens et al, 1989b;Stephens & Downes, 1990) and the other this analysis of a mammary tumour cell line, have attempted' fully to catalogue the inositol phosphate complement of any cell type.…”

Section: Concluding Commentmentioning

confidence: 99%

The inositol phosphates in WRK1 rat mammary tumour cells

Wong

Barker

Morris

et al. 1992

Biochemical Journal

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1. A detailed structural survey has been made of the inositol phosphates of unstimulated and vasopressin-stimulated WRK-1 rat mammary tumour cells. Inositol phosphate peaks were separated by h.p.l.c., and structural assignments were made for more than 20 compounds by combinations of: (a) co-chromatography with labelled standards; (b) site-specific enzymic dephosphorylation; (c) complete and partial periodate oxidation, followed by h.p.l.c. of polyols and their stereospecific oxidation by dehydrogenases; and (d) ammoniacal hydrolysis. 2. The ‘inositol monophosphates’ fraction from unstimulated cells included an uncharacterized peak, probably containing some glycerophosphoinositol, and Ins(1:2-cyclic)P. Stimulation provoked accumulation of both Ins1P and Ins3P, of Ins2P, and of Ins5P and/or the enantiomers Ins4P and Ins6P. The proportions of Ins1P and Ins3P were determined by partial periodate oxidation and enantiomeric identification of the resulting glucitols. 3. Three inositol bisphosphate peaks were detected in unstimulated cells: Ins(1,4)P2 [this was distinguished chemically from its enantiomer Ins(3,6)P2], Ins(3,4)P2 and/or Ins(1,6)P2, and Ins(4,5)P2 and/or Ins(5,6)P2. On stimulation, Ins(1,4)P2 and Ins(3,4)P2 [and/or Ins(1,6)P2] levels increased, and Ins(1:2-cyclic,4)P2 and Ins(1,3)P2 were also formed. 4. Three inositol trisphosphate peaks were obtained from unstimulated cells: all increased during stimulation. These were Ins(1,3,4)P3 [with some Ins(1:2-cyclic,4,5)P3], Ins(1,4,5)P3 and Ins(3,4,5)P3 [and/or Ins(1,5,6)P3]. During stimulation, another compound, probably Ins(1,4,6)P3, appeared in the ‘Ins(1,4,5)P3 peak’. The ‘Ins(3,4,5)P3 peak’ contained a second trisphosphate, probably Ins(2,4,5)P3. 5. Three inositol tetrakisphosphates, namely Ins(1,3,4,6)P4, Ins(1,3,4,5)P4, were present in unstimulated cells, and all accumulated during stimulation. 6. Ins(1,3,4,5,6)P5, which is the most abundant inositol polyphosphate in these cells, a less abundant inositol pentakisphosphate and inositol hexakisphosphate were all unresponsive to stimulation.

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“…Unfortunately, the latter is a rather elusive parameter. We do know that total cellular InsP 6 typically lies within the 15 ±100 mM range [22,41,42]. However, much of this InsP 6 may not be freely soluble.…”

Section: Criteria For Testing Insp 6 Function In Vitromentioning

confidence: 99%

Assessing the omnipotence of inositol hexakisphosphate

Shears

2001

Cellular Signalling

179

158

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This review assesses the authenticity of inositol hexakisphosphate (InsP(6)) being a wide-ranging regulator of many important cellular functions. Against a background in which the possible importance of localized InsP(6) metabolism is discussed, there is the facile explanation that InsP(6) is merely an "inactive" precursor for the diphosphorylated inositol phosphates. Indeed, many of the proposed cellular functions of InsP(6) cannot sustain a challenge from the implementation of a rigorous set of criteria, which are designed to avoid experimental artefacts.

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Inositol phosphates in growing and differentiating HL60 cells

Cited by 18 publications

References 0 publications

The behaviour of inositol 1,3,4,5,6-pentakisphosphate in the presence of the major biological metal cations

The behaviour of inositol 1,3,4,5,6-pentakisphosphate in the presence of the major biological metal cations

The inositol phosphates in WRK1 rat mammary tumour cells

Assessing the omnipotence of inositol hexakisphosphate

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