Inositol lipids and cell proliferation

Berridge, Michael J.; Heslop, John P.; Irvine, Robin F.; Brown, Kenneth D.

doi:10.1042/bst0130067

Cited by 87 publications

(79 citation statements)

References 23 publications

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“…FBS (5%) induced less c-fos expression but followed a similar time course (data not shown). A similar time course for c-fos induction has been observed in quiescent cells stimulated to divide by growth factors and phospholipase C agonists (7,9,35,36 …”

mentioning

confidence: 52%

Endothelin stimulates phospholipase C, Na+/H+ exchange, c-fos expression, and mitogenesis in rat mesangial cells.

Simonson¹,

Wann²,

Menè³

et al. 1989

J. Clin. Invest.

562

209

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A recently described peptide hormone, endothelin, is a potent vasoconstrictor, but it is unclear whether endothelin has other biological actions. These experiments extend the range of biological actions of endothelin to stimulation of mitogenesis. Endothelin at low concentrations (0.1-10 nM) induced mitogenesis by quiescent rat glomerular mesangial cells in culture. Mitogenesis induced by endothelin was accompanied by activation of phospholipase C with increased inositol phosphate turnover and increments of intracellular [Ca2"]. Endothelin also activated Na+/H' exchange, causing cytosolic alkalinization, and enhanced transcription of the c-fos protooncogene, additional biochemical signals closely linked to proliferation. In addition to being a vasoconstrictor, endothelin thus also functions as a mitogen, presumably through activation of phospholipase C.

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mentioning

confidence: 52%

Endothelin stimulates phospholipase C, Na+/H+ exchange, c-fos expression, and mitogenesis in rat mesangial cells.

Simonson¹,

Wann²,

Menè³

et al. 1989

J. Clin. Invest.

562

209

View full text Add to dashboard Cite

show abstract

“…Tumour promoters increase phosphatidylcholine turnover in HeLa cells (Guy & Murray, 1982). Diacylglycerols are increased in proliferating cells and are second messengers involved in the activation of protein kinase C. This, in turn, may be associated with the initiation of cellular proliferation (Berridge, 1987).…”

Section: Discussionmentioning

confidence: 99%

Abnormal phosphomonoester signals in 31P MR spectra from patients with hepatic lymphoma. A possible marker of liver infiltration and response to chemotherapy

Dixon

Angus²,

Rajagopalan³

et al. 1991

Br J Cancer

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Summary Hepatic infiltration by lymphoma can be difficult to detect by conventional methods. We have studied 22 patients in vivo 31P magnetic resonance spectroscopy of the liver and compared the results with the clinical staging and assessment of liver involvement by computed tomography (CT), ultrasound (US), and liver function tests (LFTs). We find that the phosphomonoester (PME) to ATP, and the PME to Pi ratios are the best indication of liver involvement as in all the patients with liver involvement apparent on CT or US, these ratios were elevated (> 2 s.d. above the control mean). Of the patients with deranged LFTs but normal CT or US, five out of nine showed increased PME/ATP and PME/Pi ratios, and in the patients with normal LFTs and normal CT or US, three out of eight patients had raised PME ratios. Extracts of lymphomatous lymph nodes contain high concentrations of phosphoethanolamine which suggests that this compound is responsible for the increase in the PME peak. Eleven patients were studied again after chemotherapy, and those with initially raised PME/ATP and PME/Pi ratios all showed a decrease in these ratios towards normal. The

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“…Another important metabolite that we identified was myo-inositol, the precursor of two intermediate messengers that play an important role in morphogenesis and cytogenesis, lipid synthesis, cell membrane structure and cell growth (38,39). The concentration of myo-inositol has been determined in FF by enzymatic assays (19), and it was found that the myo-inositol concentration is larger in the FF of good quality oocytes than of low quality ones.…”

Section: Discussionmentioning

confidence: 99%

NMR metabolic profile of human follicular fluid

et al. 2010

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The environment of the oocyte during its in vivo maturation consists of follicular fluid (FF) and is surrounded by granulosa cells. The FF is derived from the sanguineous plasma and secretions, synthesised in the follicle wall, that contain a large variety of growth factors, cytokines, amino acids, and other metabolites. These metabolites are presumably involved in the physiology of the oocyte. The identification, quantification and study of FF metabolites can provide additional information about the oocyte state which can be helpful in distinguishing those oocytes that have a greater capacity to be fertilised and to develop properly. The aim of this work is to identify the metabolic profile of FF samples exhaustively using High Resolution Nuclear Magnetic Resonance (NMR). A total of 30 FF samples from oocyte donors (<35 years) were analysed. Different monodimensional (1D) and bidimensional (2D) (homo and heteronuclear) NMR experiments were acquired. A total of 131 chemical shifts were assigned and 42 metabolites, including as example glucose, lactate, acetate, acetoacetate, pyruvate and b-hydroxybutyrate, were identified. High correlations were found between these important intermediaries of the energetic metabolic pathways of the follicle which can indicate the importance of these pathways in oocyte development. Some of these identified metabolites might be useful as biomarkers of the follicular maturation state, allowing oocytes with a higher fertilisation potential to be selected, thereby increasing pregnancy rates in women following in vitro fertilisation (IVF) treatments.

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Inositol lipids and cell proliferation

Cited by 87 publications

References 23 publications

Endothelin stimulates phospholipase C, Na+/H+ exchange, c-fos expression, and mitogenesis in rat mesangial cells.

Endothelin stimulates phospholipase C, Na+/H+ exchange, c-fos expression, and mitogenesis in rat mesangial cells.

Abnormal phosphomonoester signals in 31P MR spectra from patients with hepatic lymphoma. A possible marker of liver infiltration and response to chemotherapy

NMR metabolic profile of human follicular fluid

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