Inositide-specific phospholipase c β1 gene deletion in the progression of myelodysplastic syndrome to acute myeloid leukemia

Vasco, Vincenza Rita Lo; Calabrese, Giuseppe; Palka, Giandomenico; Spadano, Antonio; Morizio, Elisena; Guanciali-Franchi, Paolo; Fantasia, Donatella; Cocco, Lucio

doi:10.1038/sj.leu.2403368

Cited by 61 publications

(56 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using a specific probe for the PI-PLC-b1 gene, FISH analysis disclosed the loss of one allele of the gene in all the patients examined [Lo Vasco et al, 2004]. Rearrangements of the short arm of chromosome 20 have been detected in a number of patients with solid tumors but rarely in hematological disorders .…”

Section: Nuclear Pi-plc-b1 and Diseasementioning

confidence: 99%

“…In all five patients the 20p rearrangement was associated with the deletion of PI-PLC-b1 gene. Nevertheless the association with other chromosome aberrations hampered the definition of the role played by the 20p abnormalities in both the origin and the evolution of the disease [Lo Vasco et al, 2004].…”

Section: Nuclear Pi-plc-b1 and Diseasementioning

confidence: 99%

“…We found that the AML patients with PI-PLC-b1 gene monoallelic deletion died in a time frame ranging from 1 to 12 months and all the MDS patients with the deletion died in a time frame ranging from 1 to 6 months after developing AML. The total painting for chromosome 20 resulted normal in all of the MDS and AML patients [Lo Vasco et al, 2004]. To establish the amplitude of the deletion and the possible involvement of genes other than PI-PLC-b1 within the 20p12 region, we used a probe for another gene localized in the same band, PI-PLC-b4 gene (being the distance between the two gene as long as less than 0.1 Mb).…”

Section: Nuclear Pi-plc-b1 and Diseasementioning

confidence: 99%

“…To establish the amplitude of the deletion and the possible involvement of genes other than PI-PLC-b1 within the 20p12 region, we used a probe for another gene localized in the same band, PI-PLC-b4 gene (being the distance between the two gene as long as less than 0.1 Mb). FISH analysis revealed that all patients bearing the monoallelic deletion of PI-PLC-b1 were normal as far as PI-PLC-b4 gene was concerned, suggesting that the absence of one allele of PI-PLC-b1 gene could be due an interstitial deletion as wide as less than 0.1 Mb [Lo Vasco et al, 2004]. Immunocytochemical analysis by means of anti PI-PLC-b1 antibody, on all the AML and MDS patients that resulted normal at FISH analysis, showed normal staining of the nucleus.…”

Section: Nuclear Pi-plc-b1 and Diseasementioning

confidence: 99%

See 3 more Smart Citations

Nuclear inositol lipid metabolism: More than just second messenger generation?

Martelli

Follo

Evangelisti

et al. 2005

J of Cellular Biochemistry

View full text Add to dashboard Cite

A distinct polyphosphoinositide cycle is present in the nucleus, and growing evidence suggests its importance in DNA replication, gene transcription, and apoptosis. Even though it was initially thought that nuclear inositol lipids would function as a source for second messengers, recent findings strongly indicate that lipids present in the nucleus also fulfil other roles. The scope of this review is to highlight the most intriguing advances made in the field over the last few years, such as the possibility that nuclear phosphatidylinositol (4,5) bisphosphate is involved in maintaining chromatin in a transcriptionally active conformation, the new emerging roles for intranuclear phosphatidylinositol (3,4,5) trisphosphate and phosphoinositide 3-kinase, and the evidence which suggests a tight relationship between a decreased level of nuclear phosphoinositide specific phospholipase C-b1 and the evolution of myelodisplastic syndrome into acute myeloid leukemia.

show abstract

Section: Nuclear Pi-plc-b1 and Diseasementioning

confidence: 99%

Section: Nuclear Pi-plc-b1 and Diseasementioning

confidence: 99%

Section: Nuclear Pi-plc-b1 and Diseasementioning

confidence: 99%

Section: Nuclear Pi-plc-b1 and Diseasementioning

confidence: 99%

See 2 more Smart Citations

Nuclear inositol lipid metabolism: More than just second messenger generation?

Martelli

Follo

Evangelisti

et al. 2005

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…1 The involvement of PI-PLCb-1 in hematopoietic differentiation prompted us to investigate the role of this signaling molecule in hematological malignancies, focusing particularly on patients affected by myelodysplastic syndromes (MDS) at higher risk of evolution into acute myeloid leukemia (AML). By using fluorescence in situ hybridization (FISH) analysis, our group demonstrated, in a small number of high-risk MDS patients, 2 that subjects bearing a mono-allelic cryptic deletion of the PIPLCb-1 gene had a worse clinical outcome, as compared with patients having both alleles. In a subsequent study, it has also been shown that the expression profile of both PI-PLCb-1a and PI-PLCb-1b mRNAs, which are the two alternative splicing isoforms of PI-PLCb-1, is altered in high-risk MDS, as compared to healthy donors.…”

mentioning

confidence: 99%

PI-PLCβ-1 and activated Akt levels are linked to azacitidine responsiveness in high-risk myelodysplastic syndromes

et al. 2007

Self Cite

View full text Add to dashboard Cite

show abstract

Expression of phosphoinositide‐specific phospholipase C isoenzymes in cultured astrocytes

Vasco¹,

Fabrizi²,

Artico³

et al. 2006

J of Cellular Biochemistry

View full text Add to dashboard Cite

Signal transduction from plasma membrane to cell nucleus is a complex process depending on various components including lipid signaling molecules, in particular phosphoinositides and their related enzymes, which act at cell periphery and/or plasma membrane as well as at nuclear level. As far as the nervous system may concern the inositol lipid cycle has been hypothesized to be involved in numerous neural as well as glial functions. In this context, however, a precise panel of glial PLC isoforms has not been determined yet. In the present experiments we investigated astrocytic PLC isoforms in astrocytes obtained from foetal primary cultures of rat brain and from an established cultured (C6) rat astrocytoma cell line, two well known cell models for experimental studies on glia. Identification of PLC isoforms was achieved by using a combination of RT-PCR and immunocytochemistry experiments. While in both cell models the most represented PI-PLC isoforms were beta4, gamma1, delta4, and epsilon, isoforms PI-PLC beta2 and delta3 were not detected. Moreover, in primary astrocyte cultures PI-PLC delta3 resulted well expressed in C6 cells but was absent in astrocytes. Immunocytochemistry performed with antibodies against specific PLC isoforms substantially confirmed this pattern of expression both in astrocytes and C6 glioma cells. In particular while some isoenzymes (namely isoforms beta3 and beta4) resulted mainly nuclear, others (isoforms delta4 and epsilon) were preferentially localized at cytoplasmic and plasma membrane level.

show abstract

Inositide-specific phospholipase c β1 gene deletion in the progression of myelodysplastic syndrome to acute myeloid leukemia

Cited by 61 publications

References 15 publications

Nuclear inositol lipid metabolism: More than just second messenger generation?

Nuclear inositol lipid metabolism: More than just second messenger generation?

PI-PLCβ-1 and activated Akt levels are linked to azacitidine responsiveness in high-risk myelodysplastic syndromes

Expression of phosphoinositide‐specific phospholipase C isoenzymes in cultured astrocytes

Contact Info

Product

Resources

About