Inosine: a novel treatment for sciatic nerve injury

Cardoso, Fellipe Soares dos Santos; Martínez, Ana Maria Blanco; Almeida, Fernanda Martins de

doi:10.4103/1673-5374.286969

Cited by 2 publications

(1 citation statement)

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“…Fully understanding the speci c roles and regulatory mechanism of PNP in the brain might present therapeutic options of targeting these pathways in the future. Also, the potential bene cial effects of inosine supplementation for treatment of various central nervous system disorders have been studied since the 1970s and are well reviewed [54][55][56][57] . In summary, using the combination of hippocampal slice preparation and MSI, we introduce a technological advance that bridges the gap between live-cell imaging experiments, which have high spatial and temporal resolution but a very limited chemical dimension, and deep chemical analysis by mass spectrometry.…”

Section: Discussionmentioning

confidence: 99%

Spatially resolved metabolomics and isotope tracing reveal dynamic metabolic responses of dentate granule neurons with acute stimulation

Yellen

Miller

York

et al. 2023

Preprint

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Neuronal activity creates an intense energy demand that must be met by rapid metabolic responses. To investigate metabolic adaptations in the neuron-enriched dentate granule cell (DGC) layer within its native tissue environment, we employed murine acute hippocampal brain slices coupled with fast metabolite preservation, followed by mass spectrometry imaging (MALDI-MSI) to generate spatially resolved metabolomics and isotope tracing data. Here we show that membrane depolarization induces broad metabolic changes, including increased glycolytic activity in DGCs. Increased glucose metabolism in response to stimulation is accompanied by mobilization of endogenous inosine into pentose phosphates, via the action of purine nucleotide phosphorylase (PNP). The PNP reaction is an integral part of the neuronal response to stimulation, as inhibiting PNP leaves DGCs energetically impaired during recovery from strong activation. Performing MSI on brain slices bridges the gap between live cell physiology and the deep chemical analysis enabled by mass spectrometry.

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Section: Discussionmentioning

confidence: 99%