Inorganic pyrophosphatase activity during rice leaf senescence

Kar, M.; Mishra, D.

doi:10.1139/b75-061

Cited by 32 publications

(25 citation statements)

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“…For instance, Kisban et al (10) reported an increase in alkaline inorganic pyrophosphatase activity in detached senescing leaves of barley and wheat and Rauser (21) detected that both acid and alkaline inorganic pyrophosphatase activities declined with plant age in attached leaves of bean, tobacco, and sunflower. However, our previous investigations (7,9) suggested an opposite trend in the activities of acid and alkaline inorganic pyrophosphatase during senescence of rice leaves.…”

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confidence: 77%

“…It has been suggested that the alkaline inorganic pyrophosphatase, having extremely high affinity for pyrophosphate, is associated with the anabolic process in the leaf, while acid inorganic pyrophosphatase participates in the catabolic process (7,21,23 This investigation was undertaken to study the distribution pattern of inorganic pyrophosphatase, peroxidase, and polyphenoloxidase activities in attached leaves of different physiological ages and to determine if the changes in peroxidase and polyphenoloxidase activities during leaf senescence can be considered to be species-specific.…”

Section: Introductionmentioning

confidence: 99%

“…It has been suggested that the alkaline inorganic pyrophosphatase, having extremely high affinity for pyrophosphate, is associated with the anabolic process in the leaf, while acid inorganic pyrophosphatase participates in the catabolic process (7,21,23). However, there are several conflicting reports on increase and decrease in inorganic pyrophosphatase activity during senescence of attached and detached leaves.…”

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confidence: 99%

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Pyrophosphatase, Peroxidase and Polyphenoloxidase Activities during Leaf Development and Senescence

Patra¹,

Mishra²

1979

Plant Physiol.

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Inorganic pyrophosphatase, peroxidase, and polyphenoloxidase activities were studied as the function of leaf insertion level in eight monocotyledonous and eight dicotyledonous species. Alkaline inorganic pyrophosphatase shows a declining activity toward the end of senescence whereas no regular drift in either peroxidase or polyphenoloxidase activities was noticed during senescence of attached leaves. In the primary leaves of rice, peroxidase and polyphenoloxidase activities were high in the senescent leaves and there exists a correlation between chlorophyll content and peroxidase activity though not with polyphenoloxidase activity. Upon detachment leaves exhibit increasing peroxidase and polyphenoloxidase activities with time. The distribution of the enzyme activities during senescence of attached leaves is suggested to be species-specific, and an increase in peroxidase and polyphenoloxidase activities cannot be taken as an indicator of leaf senescence.A number of reports are available regarding the increase (1, 2, 4, 7-10, 14, 16, 19) and/or decrease (4,10,12,19,20) in activities of several hydrolyzing and oxidative enzymes during senescence of leaves. Differences in the senescence pattern of attached leaves compared to detached leaves or leaf discs have been reported (13,14,24, 25).Both acid and alkaline inorganic pyrophosphatase (EC 3.6.1.1) occur in higher plants (17). It has been suggested that the alkaline inorganic pyrophosphatase, having extremely high affinity for pyrophosphate, is associated with the anabolic process in the leaf, while acid inorganic pyrophosphatase participates in the catabolic process (7,21,23 This investigation was undertaken to study the distribution pattern of inorganic pyrophosphatase, peroxidase, and polyphenoloxidase activities in attached leaves of different physiological ages and to determine if the changes in peroxidase and polyphenoloxidase activities during leaf senescence can be considered to be species-specific. MATERIALS AND METHODSThe following species were investigated: monocotyledons: Amomum aromaticum Roxb., Eleusine corocana Gaertn., Hordeum vulgare L., Oryza sativa L., Pennisetum typhoideum Rich., Sorghum vulgare Pers., Triticum vulgare Villars., and Zea mays L.; dicotyledons: Arachis hypogaea L., Boerhaavia diffusa L., Chenopodium album L., Crotolaria striata DC., Hibiscus micranthus L., Nicotiana plumbaginifolia Viv., Raphanus sativus L., and Tabernaemontana coronaria Br.Healthy plant samples were collected on sunny days at 10 AM and the approximate age of the organs determined by the method of Kornilov et al. (11). The samples were transported to the laboratory in polyethylene bags covered with crushed ice. The leaves were numbered from the apex downward so as to obtain subsamples representative of various developmental stages. The leaves were excised within 15 min of collection, washed thoroughly with tap water, and then in distilled H20. The leaves were blotted, weighed, and stored in a refrigerator at 8 C for 30 min.Enzyme Extraction and Assay. Enz...

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mentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

“…It has been suggested that the alkaline inorganic pyrophosphatase, having extremely high affinity for pyrophosphate, is associated with the anabolic process in the leaf, while acid inorganic pyrophosphatase participates in the catabolic process (7,21,23). However, there are several conflicting reports on increase and decrease in inorganic pyrophosphatase activity during senescence of attached and detached leaves.…”

mentioning

confidence: 99%

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Pyrophosphatase, Peroxidase and Polyphenoloxidase Activities during Leaf Development and Senescence

Patra¹,

Mishra²

1979

Plant Physiol.

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“…But limited work done on the change in these two enzymes in senescing leaf tissue provide conflicting results. Kar and Mishra (1975) went to the extent the activity of considering alkaline pyrophosphatase as one of the indicator of leaf senescence. Hence, this pair of enzymes need special attention till some meaningful conclusion is drown regarding their role in the process of senescence.…”

Section: Discussionmentioning

confidence: 99%

Study of the exogenous hormonal regulation of leaf senescence in two millets, Setaria italica, l. and Pennisetum typhoides, burm

Mahapatra¹,

Kumar²,

Mohanty³

2016

IJBR

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The present project is aimed at studying the exogenous regulation of leaf senescence in two millets i.e. Setaria italica, L. and Pennisetum typhoides Burm. The present study is confined to the study of excised leaf senescence by using growth regulators as the exogenous agents. Attached leaf study was avoided for the reason that preliminary experiments showed insensitiveness of the leaves of these two millets to growth regulators in the attached condition. In this study three growth regulators were selected, each from three major groups. Benzimidazole (BZI) represented cytokinins and indole acetic acid represented the auxins and GA 3 represented Gibberelic Acid. In the present investigation, a significant negative correlation between RNA loss and RNase activity was found in both S.italica and P.typhoides. This suggests RNase like chlorophyllase responsible for bio-molecular breakdown. DNase activity was stable, it's activity increasing either in the first 72 h or up to the end of the incubation, but DNase activity decreased with time. Thus there was no correlation between the two. Acid phosphatase activity did not show any correlation with the established parameters of senescence, thus in both the plants the activity of the enzyme cannot be treated as an indicator of senescence. Among the two pyrophosphatases, alkaline enzyme showed a parallelism with the changes in chlorophyll and can be used as a reliable indicator of the process. On the other hand acid inorganic phosphatase activity increased during the incubation, but had no significant relation with other senescence related changes, suggesting more investigations to ascertain the role of acid inorganic pyrophosphatase.

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“…The use of flower preservative decreased the activity of both pyrophosphatases in roses, did not change it in chrysanthemums, and maintained the alkaline enzyme at essentially the initial level in carnations. The activities of both enzymes were generally greater in the flowers of carnations and chrysanthemums than in roses, excepting the acid pyrophosphatase in uncut chrysanthemums on day 15, or the alkaline pyrophosphatase on days 15 Table 1); part of the sample was used for dry (Kar and Mishra 1975 …”

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confidence: 99%

Acid and Alkaline Inorganic Pyrophosphatases in Senescing Flowers of Rose, Carnation and Chrysanthemum

Parups¹

1976

Can. J. Plant Sci.

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Inorganic pyrophosphatase activity during rice leaf senescence

Cited by 32 publications

References 14 publications

Pyrophosphatase, Peroxidase and Polyphenoloxidase Activities during Leaf Development and Senescence

Pyrophosphatase, Peroxidase and Polyphenoloxidase Activities during Leaf Development and Senescence

Study of the exogenous hormonal regulation of leaf senescence in two millets, Setaria italica, l. and Pennisetum typhoides, burm

Acid and Alkaline Inorganic Pyrophosphatases in Senescing Flowers of Rose, Carnation and Chrysanthemum

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