Inorganic Polyphosphate and Physiological Properties of Saccharomyces cerevisiae Yeast Overexpressing Ppn2

Ryazanova, L. P.; Ledova, L. A.; Andreeva, N. A.; Zvonarev, Anton; Eldarov, M. A.; Kulakovskaya, T. V.

doi:10.1134/s0006297920040124

Cited by 7 publications

(5 citation statements)

References 21 publications

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“…There is potential for this because poly(P) has a significant role for the storage of cations, such as for Zn 2+ , Ca 2+ , Mn 2+ , and Mg 2+ ( 96 – 99 ), and also for cation uptake, as shown for Mg 2+ ( 100 ). Furthermore, poly(P) may affect cellular signaling, influencing the stress response ( 101 – 104 ). Here, it may even have direct impact, such as through polyphosphorylation of lysine residues, which modifies yeast topoisomerase 1 (Tpo1) and nuclear signal recognition factor 1 (Nsr1) ( 105 , 106 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolic Consequences of Polyphosphate Synthesis and Imminent Phosphate Limitation

Kim

Qiu

Jessen

et al. 2023

mBio

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Section: Discussionmentioning

confidence: 99%

Metabolic Consequences of Polyphosphate Synthesis and Imminent Phosphate Limitation

Kim

Qiu

Jessen

et al. 2023

mBio

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“…There is potential for this because polyP has a significant role for the storage of cations, such as for Zn 2+ , Ca 2+ , Mn 2+ and Mg 2+ (88–91), but also for cation uptake, as shown for Mg 2+ (92). Furthermore, polyP may affect cellular signaling, influencing the stress response (93–96). Here, it may even have direct impact, such as through polyphosphorylation of lysine residues, which modifies yeast topoisomerase 1 (Tpo1) and nuclear signal recognition factor 1 (Nsr1) (97, 98).…”

Section: Discussionmentioning

confidence: 99%

Metabolic consequences of polyphosphate synthesis and imminent phosphate limitation

Kim

Qiu

Jessen

et al. 2022

Preprint

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Cells stabilize intracellular inorganic phosphate (Pi) to compromise between large biosynthetic needs and detrimental bioenergetic effects of Pi. Pihomeostasis in eukaryotes employs SPXs domains, which are receptors for inositol pyrophosphates. We explored how polymerization and storage of Pi in acidocalcisome-like vacuoles supports S. cerevisiae metabolism and how these cells recognize Piscarcity. Whereas Pistarvation affects numerous metabolic pathways, beginning Piscarcity affects few metabolites. These include inositol pyrophosphates and ATP, a low-affinity substrate for inositol pyrophosphate-synthesizing kinases. Declining ATP and inositol pyrophosphates may thus be indicators of impending Pilimitation. Actual Pistarvation triggers accumulation of the purine synthesis intermediate 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), which activates Pi-dependent transcription factors. Cells lacking polyphosphate show Pistarvation features already under Pi-replete conditions, suggesting that vacuolar polyphosphate supplies Pifor metabolism even when Piis abundant. However, polyphosphate deficiency also generates unique metabolic changes that are not observed in starving wildtype cells. Polyphosphate in acidocalcisome-like vacuoles may hence be more than a global phosphate reserve and channel Pito preferred cellular processes.

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“…Recent studies have shown that overexpression of OsGAPB in rice under low light stress increases stress tolerance and reduces the effect of low yield caused by low light stress [46]. The expression of PPN2 gene in yeast is controlled by the strong constitutive promoter of glyceraldehyde 3-phosphate dehydrogenase gene (PKG1), resulting in a significant increase in cobalt/zinc ion-stimulated cohesive phosphatase activity, and cells overexpressing PPN2 are more resistant to peroxides and alkali [47]. TaGAPC5/6 genes were found to respond positively to drought stress through reactive oxygen species (ROS) scavenging and stomatal movement in wheat.…”

Section: Members Of Gapdh Family Participate In Plant Stress Resistancementioning

confidence: 99%

Analysis of SI-Related BoGAPDH Family Genes and Response of BoGAPC to SI Signal in Brassica oleracea L.

Xie

Zhang

et al. 2021

Genes

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Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is not only involved in carbohydrate metabolism, but also plays an important role in stress resistance. However, it has not been reported in Brassica oleracea. In this study, we performed a genome-wide identification of BoGAPDH in B. oleracea and performed cloning and expression analysis of one of the differentially expressed genes, BoGAPC. A total of 16 members of the BoGAPDH family were identified in B. oleracea, which were conserved, distributed unevenly on chromosomes and had tandem repeat genes. Most of the genes were down-regulated during self-pollination, and the highest expression was found in stigmas and sepals. Different transcriptome data showed that BoGAPDH genes were differentially expressed under stress, which was consistent with the results of qRT-PCR. We cloned and analyzed the differentially expressed gene BoGAPC and found that it was in the down-regulated mode 1 h after self-pollination, and the expression was the highest in the stigma, which was consistent with the result of GUS staining. The promoter region of the gene not only has stress response elements and plant hormone response elements, but also has a variety of specific elements for regulating floral organ development. Subcellular localization indicates that the BoGAPC protein is located in the cytoplasm and belongs to the active protein in the cytoplasm. The results of prokaryotic expression showed that the size of the BoGAPC protein was about 37 kDa, which was consistent with the expected results, indicating that the protein was induced in prokaryotic cells. The results of yeast two-hybrid and GST pull-down showed that the SRK kinase domain interacted with the BoGAPC protein. The above results suggest that the BoGAPDH family of B. oleracea plays an important role in the process of plant stress resistance, and the BoGAPC gene may be involved in the process of self-incompatibility in B. oleracea, which may respond to SI by encoding proteins directly interacting with SRK.

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Inorganic Polyphosphate and Physiological Properties of Saccharomyces cerevisiae Yeast Overexpressing Ppn2

Cited by 7 publications

References 21 publications

Metabolic Consequences of Polyphosphate Synthesis and Imminent Phosphate Limitation

Metabolic Consequences of Polyphosphate Synthesis and Imminent Phosphate Limitation

Metabolic consequences of polyphosphate synthesis and imminent phosphate limitation

Analysis of SI-Related BoGAPDH Family Genes and Response of BoGAPC to SI Signal in Brassica oleracea L.

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