Inorganic phosphate uptake in intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don under varying Pi status

Ohnishi, Miwa; Mimura, Tetsuro; Tsujimura, Tomoko; Mitsuhashi, Naoto; Washitani-Nemoto, Setsuko; Maeshima, Masayoshi; Martinoia, Enrico

doi:10.1007/s00425-006-0379-9

Cited by 14 publications

(5 citation statements)

References 26 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was also observed with MeP. Our results contrast with the activation, under low-Pi status, of Pi uptake into purified vacuoles of Catharanthus roseus culture cells (Ohnishi et al, 2007). This discrepancy may be due to the fact that the cyt solPi concentration is much lower than the concentration of Pi added to the vacuole suspension medium by the authors.…”

Section: Pi Influx Into the Vacuolecontrasting

confidence: 95%

Phosphate (Pi) Starvation Effect on the Cytosolic Pi Concentration and Pi Exchanges across the Tonoplast in Plant Cells: An in Vivo 31P-Nuclear Magnetic Resonance Study Using Methylphosphonate as a Pi Analog

et al. 2009

View full text Add to dashboard Cite

In vivo 31P-NMR analyses showed that the phosphate (Pi) concentration in the cytosol of sycamore (Acer pseudoplatanus) and Arabidopsis (Arabidopsis thaliana) cells was much lower than the cytoplasmic Pi concentrations usually considered (60–80 μm instead of >1 mm) and that it dropped very rapidly following the onset of Pi starvation. The Pi efflux from the vacuole was insufficient to compensate for the absence of external Pi supply, suggesting that the drop of cytosolic Pi might be the first endogenous signal triggering the Pi starvation rescue metabolism. Successive short sequences of Pi supply and deprivation showed that added Pi transiently accumulated in the cytosol, then in the stroma and matrix of organelles bounded by two membranes (plastids and mitochondria, respectively), and subsequently in the vacuole. The Pi analog methylphosphonate (MeP) was used to analyze Pi exchanges across the tonoplast. MeP incorporated into cells via the Pi carrier of the plasma membrane; it accumulated massively in the cytosol and prevented Pi efflux from the vacuole. This blocking of vacuolar Pi efflux was confirmed by in vitro assays with purified vacuoles. Subsequent incorporation of Pi into the cells triggered a massive transfer of MeP from the cytosol to the vacuole. Mechanisms for Pi exchanges across the tonoplast are discussed in the light of the low cytosolic Pi level, the cell response to Pi starvation, and the Pi/MeP interactive effects.

show abstract

Section: Pi Influx Into the Vacuolecontrasting

confidence: 95%

Phosphate (Pi) Starvation Effect on the Cytosolic Pi Concentration and Pi Exchanges across the Tonoplast in Plant Cells: An in Vivo 31P-Nuclear Magnetic Resonance Study Using Methylphosphonate as a Pi Analog

et al. 2009

View full text Add to dashboard Cite

show abstract

“…A study with suspension‐cultured cells of Catharanthus roseus revealed that H + ‐pumping activities of vacuolar H + ‐PPase and H + ‐ATPase were activated under Pi limiting conditions. Interestingly, Western blot analysis revealed no change in protein abundance [107]. Similar results were obtained with rye plants grown under conditions where the mineral nutrients were diluted 1000 fold.…”

Section: Role Of Plant H+‐ppases In Growth and Developmentsupporting

confidence: 80%

Plant proton pumps

2007

View full text Add to dashboard Cite

Chemiosmotic circuits of plant cells are driven by proton (H + ) gradients that mediate secondary active transport of compounds across plasma and endosomal membranes. Furthermore, regulation of endosomal acidification is critical for endocytic and secretory pathways. For plants to react to their constantly changing environments and at the same time maintain optimal metabolic conditions, the expression, activity and interplay of the pumps generating these H + gradients have to be tightly regulated. In this review, we will highlight results on the regulation, localization and physiological roles of these H + -pumps, namely the plasma membrane H + -ATPase, the vacuolar H + -ATPase and the vacuolar H + -PPase.

show abstract

“…Increased rhizosphere acidification is a consistent phenotype of transgenic plants expressing H + ‐PPase genes, and is likely to contribute to the increased Pi availability and the observed higher Pi uptake (Gaxiola et al ., ; Pei et al ., ; Yang et al ., ). Further support for a role of H + ‐PPases under Pi starvation comes from earlier work in Brassica and other species (Kasai et al ., ; Palma et al ., ; Ohnishi et al ., ), and it has also been shown that H + ‐PPases are a component of PSR, as they were upregulated in OsPTF1 overexpression lines (Yi et al ., ).…”

Section: The H+‐pyrophosphatase Avp1: a Gene With Multiple Benefitsmentioning

confidence: 99%

Improving phosphorus use efficiency: a complex trait with emerging opportunities

et al. 2017

View full text Add to dashboard Cite

SUMMARYPhosphorus (P) is one of the essential nutrients for plants, and is indispensable for plant growth and development. P deficiency severely limits crop yield, and regular fertilizer applications are required to obtain high yields and to prevent soil degradation. To access P from the soil, plants have evolved high-and low-affinity Pi transporters and the ability to induce root architectural changes to forage P. Also, adjustments of numerous cellular processes are triggered by the P starvation response, a tightly regulated process in plants. With the increasing demand for food as a result of a growing population, the demand for P fertilizer is steadily increasing. Given the high costs of fertilizers and in light of the fact that phosphate rock, the source of P fertilizer, is a finite natural resource, there is a need to enhance P fertilizer use efficiency in agricultural systems and to develop plants with enhanced Pi uptake and internal P-use efficiency (PUE). In this review we will provide an overview of continuing relevant research and highlight different approaches towards developing crops with enhanced PUE. In this context, we will summarize our current understanding of root responses to low phosphorus conditions and will emphasize the importance of combining PUE with tolerance of other stresses, such as aluminum toxicity. Of the many genes associated with Pi deficiency, this review will focus on those that hold promise or are already at an advanced stage of testing (OsPSTOL1, AVP1, PHO1 and OsPHT1;6). Finally, an update is provided on the progress made exploring alternative technologies, such as phosphite fertilizer.

show abstract

Inorganic phosphate uptake in intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don under varying Pi status

Cited by 14 publications

References 26 publications

Phosphate (Pi) Starvation Effect on the Cytosolic Pi Concentration and Pi Exchanges across the Tonoplast in Plant Cells: An in Vivo 31P-Nuclear Magnetic Resonance Study Using Methylphosphonate as a Pi Analog

Phosphate (Pi) Starvation Effect on the Cytosolic Pi Concentration and Pi Exchanges across the Tonoplast in Plant Cells: An in Vivo 31P-Nuclear Magnetic Resonance Study Using Methylphosphonate as a Pi Analog

Plant proton pumps

Improving phosphorus use efficiency: a complex trait with emerging opportunities

Contact Info

Product

Resources

About